Hitachi F7000 Instruction Manual
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1.7 Input of Spectrophotometer Serial No. 1 - 30 1.7 Input of Spectrophotometer Serial No. The serial No. of the presently connected F-7000 fluorescence spectrophotometer can be displayed for its confirmation. Select the Instrument command from the Tools menu or click the button. The dialog box shown in Fig. 1-44 opens. The serial No. of the connected F-7000 fluorescence spectrophotometer can be checked here. Fig. 1-44
1.8 1 - 31 1.8 Window Setting The information to be displayed upon starting the FL Solutions 2.1 program or opening the data processing window can be selected here by excluding unnecessary one from display. Fig. 1-45 Options Setting Window 1 Select Options command from the Tools menu. 2 The Options setting window is now displayed. In this window, the following settings can be made. After your setting, click OK. • The set functions are validated when starting the monitor window again or opening the data processing window. The Options setting window has the following tabs. Display tab Fonts tab Processing tab Colors tab Startup tab Sensitivity Slits tab Analysis condition tab Report tab
1.8 Window Setting 1 - 32 1.8.1 Display Tab Items to be displayed when opening a saved data file or data immediately after measurement can be set here. Those items can also be set by tool buttons or View menu after measurement. For the settable range of each parameter, refer to Appendix I. Fig. 1-46 Display Tab 1 Show trace display Put a check mark in this box, and the trace cursor appears to permit spectrum tracing when the data processing window is open. 2 Show gridlines with increment When a check mark is put in this box, the contents of monitor and data processing windows will be displayed with ruled lines. The width of ruled line is selectable. 3 Wavelength scan Items to be displayed on the data processing window open in the wavelength scan mode can be set. 4 Time scan Items to be displayed on the data processing window open in the time scan mode can be set. 5 3-D Scan Items to be displayed on the data processing window open in the 3-D scan mode can be set. 6 Intracellular cation scan Items to be displayed on the data processing window open in the intercellular cation scan mode can be set. 7 Intracellular cation spectrum Items to be displayed on the data processing window open in the intercellular cation spectrum mode can be set. 1 3 5 7 8 2 4 6 9
1.8.1 1 - 33 8 Polarization time scan Items to be displayed on the data processing window open in the polarization time scan mode can be set. 9 Polarization spectrum Items to be displayed on the data processing window open in the polarization spectrum mode can be set.
1.8 Window Setting 1 - 34 1.8.2 Fonts Tab Display font, character size, etc. can be set. Printer font, character size, etc. are also settable. Fig. 1-47 Fonts Tab 1 Display Font Font, character size, etc. in the spectrum area under display can be set. 2 Printer Font Printer font is settable. 3 Clicking Select presents a window as shown in Fig. 1-48. Fig. 1-48 Font Specification 4 Set the desired font, style and size. 1 2 4 3
1.8.3 1 - 35 1.8.3 Processing Tab Initial parameters of data processing can be set. For the settable range of each parameter, refer to Appendix I. Fig. 1-49 Processing Tab 1 Area integration method An area integration method can be selected. 2 Calibration A calibration curve formula to be used for the Photometry mode can be set. 3 Peak table/Calculate peak area By means of a check mark, inclusion of the area calculation item in the peak table can be selected or avoided. 4 Smooth The initial values of smoothing parameters can be set. However, this setting will not be reflected on the parameters of Analysis Method window. 5 Derivative With regard to derivative operation, the initial parameters of smoothing in an analysis method or to be carried out after measurement can be set. 1 2 3 4 5
1.8 Window Setting 1 - 36 1.8.4 Colors Tab The color of the spectrum to be overlaid is settable here. Color 1 (bottom) is the color of the initial display. Fig. 1-50 Colors Tab 1 Click Select…. • A window as shown in Fig. 1-51 is presented. Fig. 1-51 Color Setting 2 Select a basic color or custom color. 3 If there is no basic color you want, click Define Custom Colors. • A window as shown in Fig. 1-52 is presented. 1 3 2
1.8.4 1 - 37 Fig. 1-52 Addition of Color 4 Select hue, saturation, luminance, etc. and click Add to Custom Colors. 5 Your color is then added to the custom colors. So select it and click OK. 5 4
1.8 Window Setting 1 - 38 1.8.5 Startup Tab Analysis method to be taken upon start of the FL Solutions 2.1 program is settable here. Fig. 1-53 Startup Tab 1 Restore last method used The method used last will be brought in. 2 Auto Parameter File The specified method will be brought in. 3 For specifying a method, click this button. • A window as shown in Fig. 1-54 is then presented. Fig. 1-54 4 Specify a method file and click Open. The specified method file appears in Fig. 1-53. 1 2 3 4
1.8.6 1 - 39 1.8.6 Sensitivity Slits Tab Fig. 1-55 Sensitivity Slits Tab Sensitivity Slits A slit width is settable for sensitivity check. For sensitivity check, select the Sensitivity Check command from the Utility menu.