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Hitachi F7000 Instruction Manual

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    							1.7    Input of Spectrophotometer Serial No. 
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    1.7    Input of Spectrophotometer Serial No.   
     
    The serial No. of the presently connected F-7000 fluorescence spectrophotometer can be displayed 
    for its confirmation.   
    Select the Instrument command from the Tools menu or click the 
     button.  The dialog box shown 
    in Fig. 1-44 opens.    The serial No. of the connected F-7000 fluorescence spectrophotometer can be 
    checked here.   
     
     
     
    Fig. 1-44   
     
      
    						
    							1.8 
    1 - 31 
    1.8  Window Setting  
     
    The information to be displayed upon starting the FL Solutions 2.1 program or opening the data 
    processing window can be selected here by excluding unnecessary one from display.   
     
     
    Fig. 1-45    Options Setting Window 
     
    1 Select Options command from the 
    Tools menu.   
    2 The Options setting window is now 
    displayed.    In this window, the 
    following settings can be made.   
    After your setting, click OK.  
    • The set functions are validated 
    when starting the monitor window 
    again or opening the data 
    processing window.   
     
    The Options setting window has the 
    following tabs.   
    Display tab 
    Fonts tab   
    Processing tab   
    Colors tab   
    Startup tab 
    Sensitivity Slits tab   
    Analysis condition tab   
    Report tab   
     
      
    						
    							1.8  Window Setting 
    1 - 32 
    1.8.1  Display Tab  
     
    Items to be displayed when opening a saved data file or data immediately after measurement can be 
    set here.    Those items can also be set by tool buttons or View menu after measurement.   
    For the settable range of each parameter, refer to Appendix I.   
     
     
    Fig. 1-46  Display Tab 
     
    1 Show trace display   
    Put a check mark in this box, and the trace 
    cursor appears to permit spectrum tracing 
    when the data processing window is open.   
    2 Show gridlines with increment   
    When a check mark is put in this box, the 
    contents of monitor and data processing 
    windows will be displayed with ruled lines.   
    The width of ruled line is selectable.   
    3 Wavelength scan   
    Items to be displayed on the data 
    processing window open in the wavelength 
    scan mode can be set.   
    4 Time scan   
    Items to be displayed on the data 
    processing window open in the time scan 
    mode can be set.   
    5 3-D Scan   
    Items to be displayed on the data 
    processing window open in the 3-D scan 
    mode can be set.   
    6 Intracellular cation scan   
    Items to be displayed on the data 
    processing window open in the intercellular 
    cation scan mode can be set.   
    7 Intracellular cation spectrum   
    Items to be displayed on the data 
    processing window open in the intercellular 
    cation spectrum mode can be set.   
    1 
    3 
    5 
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    8 
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    6 
    9  
    						
    							1.8.1 
    1 - 33 
    8 Polarization time scan   
    Items to be displayed on the data 
    processing window open in the polarization 
    time scan mode can be set.   
    9 Polarization spectrum   
    Items to be displayed on the data 
    processing window open in the polarization 
    spectrum mode can be set.   
      
    						
    							1.8  Window Setting 
    1 - 34 
    1.8.2  Fonts Tab  
     
    Display font, character size, etc. can be set.   
    Printer font, character size, etc. are also settable.   
     
     
    Fig. 1-47  Fonts Tab  
     
    1 Display Font   
    Font, character size, etc. in the 
    spectrum area under display can be 
    set.  
    2 Printer Font   
    Printer font is settable.   
    3 Clicking Select presents a window as 
    shown in Fig. 1-48.     
     
     
     
     
    Fig. 1-48  Font Specification  
     
    4 Set the desired font, style and size.     
     
    1 
    2 
    4 
    3 
    						
    							1.8.3 
    1 - 35 
    1.8.3  Processing Tab  
     
    Initial parameters of data processing can be set.    For the settable range of each parameter, refer to 
    Appendix I.   
     
     
    Fig. 1-49  Processing Tab 
     
    1 Area integration method   
    An area integration method can be 
    selected.  
    2 Calibration  
    A calibration curve formula to be used 
    for the Photometry mode can be set.   
    3 Peak table/Calculate peak area   
    By means of a check mark, inclusion 
    of the area calculation item in the 
    peak table can be selected or avoided.   
    4 Smooth  
    The initial values of smoothing 
    parameters can be set.    However, 
    this setting will not be reflected on the 
    parameters of Analysis Method 
    window.  
    5 Derivative  
    With regard to derivative operation, 
    the initial parameters of smoothing in 
    an analysis method or to be carried 
    out after measurement can be set.   
     
    1 
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    3
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    5  
    						
    							1.8  Window Setting 
    1 - 36 
    1.8.4  Colors Tab  
     
    The color of the spectrum to be overlaid is settable here.    Color 1 (bottom) is the color of the initial 
    display.  
     
     
    Fig. 1-50  Colors Tab  
     
    1 Click Select….  
    • A window as shown in Fig. 1-51 is 
    presented.  
     
     
    Fig. 1-51  Color Setting  
    2 Select a basic color or custom color.   
    3 If there is no basic color you want, click 
    Define Custom Colors.  
    • A window as shown in Fig. 1-52 is 
    presented.  
     
    1 
    3 
    2  
    						
    							1.8.4 
    1 - 37 
     
     
     
    Fig. 1-52    Addition of Color   
     
    4 Select hue, saturation, luminance, etc. and 
    click Add to Custom Colors.  
    5 Your color is then added to the custom 
    colors.    So select it and click OK.  
     
    5 4 
    						
    							1.8  Window Setting 
    1 - 38 
    1.8.5  Startup Tab 
     
    Analysis method to be taken upon start of the FL Solutions 2.1 program is settable here.   
     
     
    Fig. 1-53  Startup Tab 
     
    1 Restore last method used   
    The method used last will be brought 
    in.  
    2 Auto Parameter File   
    The specified method will be brought 
    in.  
    3 For specifying a method, click this 
    button.  
    • A window as shown in Fig. 1-54 is 
    then presented.   
     
     
     
    Fig. 1-54   
     
    4 Specify a method file and click Open.  
    The specified method file appears in 
    Fig. 1-53.   
     
     
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    							1.8.6 
    1 - 39 
    1.8.6  Sensitivity Slits Tab  
     
     
    Fig. 1-55    Sensitivity Slits Tab   
     Sensitivity Slits   
    A slit width is settable for sensitivity 
    check.  
    For sensitivity check, select the 
    Sensitivity Check command from the 
    Utility menu.   
     
      
    						
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