Hitachi F7000 Instruction Manual

							3.2    Creating an Analysis Method 
3 - 6 
3 EX WL   
Enter an excitation wavelength.   
4 EM WL   
Enter an emission wavelength.   
5 Time unit   
Set a time unit.   
6 Scan time   
Set a wavelength scan speed.   
For the relationship between scan time and sampling interval, refer 
to Appendix H.   
7 Delay  
After pressing the Measure button, measurement is started 
following the delay time set here.    It is used for temperature 
stabilization, etc.    In repeat measurement, it is the time until the 
start of the first measurement.    The delay time is invalid for the 
second and subsequent measurements.   
8 EX Slit   
Select a slit width on the excitation side.   
9 EM Slit   
Select a slit width on the emission side.   
10 PMT Voltage   
A function for controlling the voltage of the photomultiplier detector 
(photomultiplier tube).    As a reference, changing from 400 V to 
700 V increases the data value about two digits and changing from 
700 V to 950 V increases it about 1 digit.   
When a check mark is put for PMT Voltage 0-1000V, setting is 
allowed within a range from 0 to 1,000 V.   
11 PMT Voltage 0-1000V   
When a check mark is put in this box, the photomultiplier voltage is 
settable within a range from 0 to 1000 V.   
12 Response  
Set a response time.   
13 Shutter control   
The shutter can automatically be closed in other than measurement 
for slowing down sample deterioration due to the energy of 
excitation beam and opened when measurement starts.      
						

							3.2.2 
3 - 7 
14 Replicates  
Set the number of repeat measurements.     
15 Cycle time   
Set a repetition interval.   
16 Stopped Flow   
When a check mark is put in this box, the stopped flow mode is set.   
In this case, when you click the measurement start button, the 
system waits until an external start signal comes in.     
Then, measurement starts.   
 
  
						

							3.2    Creating an Analysis Method 
3 - 8 
When selecting the Monitor tab, the window in Fig. 3-5 appears.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 3-5  Monitor Tab 
 
 
Assign the following parameters.   
 
1 Y-Axis Max (maximum)   
Enter a maximum value for Y-axis on the monitor window.   
2 Y-Axis Min (minimum)   
Enter a minimum value for Y-axis on the monitor window.   
3 X-Axis Max (maximum)   
Enter a maximum value for X-axis on the monitor window.   
4 X-Axis Min (minimum)   
Enter a minimum value for X-axis on the monitor window.   
 
NOTE:  X-Axis Max and Min correspond to a display interval on the 
monitor window.    For example, when Max and Min are 60 
and 30, display on the window is made within 30 to 60 s, 60 to 
90 s, 90 to 120 s and so on.   
3.2.3 Monitor Tab 
 
6 
7 
8 
5 
2 
1 
4
3 
						

							3.2.3 
3 - 9 
5 Open data processing window after data acquisition   
Select whether or not to conduct data processing after sample 
measurement.    When selected (check mark is applied), an icon 
for this function (as shown in 
) is displayed.   
 
NOTE:  When the remaining memory capacity of PC in operation is 
very small (system resource is less than 20%), the data 
processing window will not open even if this item is turned ON.   
Either close the presently opened data processing window or 
restart the FL Solutions program.   
 
6 Open data processing window after acquisition (Overlay)   
Select whether or not to conduct data processing with spectra 
overlaid after sample measurement.    This setting is unavailable in 
repeat measurement.   
7 Print report after data acquisition   
Select whether or not to print a report after sample measurement.   
When selected (check mark is applied), printing will be made 
automatically after measurement.    The items set at the Report tab 
will be printed.     
8 Overlay  
Spectra can be overlaid in the monitor window.   
This setting cannot be selected for repeat measurement.   
 
  
						

							3.2    Creating an Analysis Method 
3 - 10 
On selection of the Processing tab, the window in Fig. 3-6 will be 
displayed.  
For the settable range of each parameter, refer to Appendix G.   
 
 
Fig. 3-6  Processing Tab  
 
 
1 Processing choices   
A list of data processing is displayed.    Select a data processing 
item, and click the Right arrow button (
).  Then, the selected 
method appears in the Processing steps field.    For example, 
select ‘Derivative’ and click 
, then ‘Derivative’ is displayed in 
the Processing steps field as shown in Fig. 3-7.   
 
 
Fig. 3-7 
3.2.4 Processing 
Tab 
 
3 
7 
6 
1 2
8 
5 
4  
						

							3.2.4 
3 - 11 
2 Processing steps   
The processing sequence is displayed.    After measurement, data 
processing will be carried out in the order set here.   
How to Delete a Processing Method   
Select a processing method to be deleted and click the Left 
arrow button (
).    Then, the selected method disappears 
from the Processing steps field.   
 
Display and Change of Parameter   
When you click [+] in the Processing steps field, a parameter list 
is displayed.    Click a parameter name to be changed, and it 
becomes changeable.    For changing a ‘Derivative’ parameter, 
click [+] at the left of ‘Derivative’ in Fig. 3-7.    The present 
derivative parameters (Derivative order, Smoothing order, 
Number of points) are displayed.    For changing the derivative 
order, click ‘Derivative order,” and it is highlighted as shown in 
Fig. 3-8 to allow your change.   
 
 
 
Fig. 3-8 
 
 
3 Peak Finding:  Integration method  
Select an integration method for the peaks to be displayed in the 
data processing window.   
4 Peak Finding:  Threshold  
Set a detection limit for the photometric value axis of peak and 
valley.  
Peaks or valleys below the set value will not be detected.      
						

							3.2    Creating an Analysis Method 
3 - 12 
5 Peak Finding:  Sensitivity  
Select the number of data points in the horizontal axis direction 
(abscissa).    For detecting sharp peaks, select a sensitivity value of 
“1.”    Or select “8” for detecting broad peaks.   
6 Kinetics:  Start time  
Enter a start time for kinetics (rate calculation).   
7 Kinetics:  End time  
Enter an end time for kinetics.   
8 Kinetics:  K-Factor  
Enter a K-factor for kinetics.   
 
  
						

							3.2.5 
3 - 13 
Select the Report tab, and the window in Fig. 3-9 will appear.   
 
 
 
Fig. 3-9  Report Tab 
 
 
This window is used to select a printout method and items of 
measurement results.   
 
1 Output  
• Print Report   
A report will be printed.    For printing method, refer to 3.5.   
• Use Microsoft(R) Excel   
Data will be transferred to Microsoft(R) Excel.    For transferring 
method, refer to 7.13.   
• Use print generator sheet   
Select this method for using the report generator (option).     
For usage, refer to the instruction manual of the report 
generator.  
2 Orientation  
Selectable when Print Report is chosen for Output.   
Select a printing orientation.    Either Portrait or Landscape 
orientation is selectable.    When the Landscape orientation is 
selected, date and method can be printed.   
3.2.5 Report Tab 
 
4 
3 
1 
2 
						

							3.2    Creating an Analysis Method 
3 - 14 
3 Print items (printable/transferable items) 
• Include date   
The date and time of analysis can be printed.    Printing time will 
always be printed.   
• Include method   
• Include graph   
• Include data listing   
◊ Constant  
Data will be printed at fixed intervals between 2 wavelength 
points.    Make entries for Data interval, Data start and Data 
end.  
◊ Select data  
Data at the specified wavelength will be printed.    Up to 12 
time points can be specified.   
When clicking a time value, it allows editing.    Select a time 
within the measurable range set in the Instrument tab.   
• Include peak table   
Select output items in the peak table.   
When a check mark is put in this box, the following output items 
are selectable.   
◊  Peak time/Peak data   
◊  Start time/End time   
◊  Valley time/Valley data   
◊ Peak Area  
• Include kinetics   
4 Printer Font   
Click the Select button for changing the front to be used in the 
report.  
  
						

							3.3.1 
3 - 15 
3.3  Defining Your Samples  
 
When “Use sample table” is not selected in Analysis Method, a sample 
must be defined in the following procedure before measurement.   
 
(1) Click the 
  button.    The window in Fig. 3-10 will appear.   
 
 
 
Fig. 3-10   
 
 
(2)  Make entries for Sample name and Comment.   
Maximum number of enterable characters (half-size)   
Sample name :  40 characters   
Comment : 255 characters  
 
For automatic saving, put a check mark in the “Auto File” box to 
specify a file name.    First click the Select button, and then 
specify a saving location and enter a file name.   
When you put a check mark at the head of “Auto JCAMP-DX file,” 
“Auto Text file” and “Auto Meta file” after turning on “Auto File,” 
time scan data can be saved automatically in the following file 
formats as soon as this data is automatically saved.   
•  JCAMP-DX file (*.dx)   
•  ASCII text file (*.txt)   
•  Graph meta file (*.wmf)   
The data will be saved in the file name entered for “File name.”   
 
(3)  After completion of setting, click OK.  
 
3.3.1 Without Using 
Sample Table