Hitachi F7000 Instruction Manual

							 
SAFETY - 1 
 SAFETY SUMMARY 
 
 
 
 
 
PRECAUTIONS ON SAFETY 
 
Before using the F-7000 fluorescence spectrophotometer, be sure to 
read the following safety instructions carefully. 
 
 
     General Safety Guidelines 
 
  Follow all the operating procedures provided in this manual. 
 Installation and maintenance of this instrument shall be carried 
out by service personnel qualified for this purpose. 
  Be sure to observe the warnings indicated on the product and in the 
instruction manual.    Failure to do so could result in personal injury 
or damage to the product. 
  The hazard warnings which appear on the warning labels on the 
product or in the manual have one of the following alert headings 
consisting of an alert symbol and signal word DANGER, WARNING, 
or CAUTION. 
 
DANGER  :  Indicates an imminently hazardous situation 
which, if not avoided, will result in death or 
serious injury.   
(This warning does not apply to this product.) 
WARNING  :  Indicates a potentially hazardous situation 
which, if not avoided, can result in death or 
serious injury. 
CAUTION :  Indicates a hazardous situation which, if not 
avoided, will or can result in minor or 
moderate injury, or serious damage to the 
product. 
 :  The alert symbol shown at left precedes 
every signal word for hazard warnings, and 
appears in safety-related descriptions in the 
manual. 
 
“NOTE” and “NOTICE” are heading words which do not concern 
personal safety directly. 
  
						

							 
SAFETY - 2 
 SAFETY SUMMARY 
 
 
 
 
 
     General Safety Guidelines (Continued) 
 
NOTICE  :  Used to indicate an instruction for preventing damage 
to the product. 
NOTE  :  Used to indicate an instruction for ensuring correct 
use of the product and accurate analysis therewith. 
 
 
  Do not modify the instrument, replace parts that are not user-
serviceable, use non-specified parts, nor remove safety devices, as it 
could be hazardous.   
  Installation at delivery, maintenance and relocation should be 
referred to service personnel qualified by us.   
  Do not perform any operation or action other than described in this 
manual.    When in doubt, please contact our local sales 
representative or service office nearest you.   
  When using a chemical for analytical operation, be sure to provide 
proper ventilation in the laboratory room as per local requirements.   
Inadequate ventilation could endanger your health.   
  Keep in mind that the hazard warnings in this manual or on the 
product cannot cover every possible case, as it is impossible to 
predict and evaluate all circumstances beforehand.   
Be alert and use your common sense.   
 
  
						

							 
SAFETY - 3 
 SAFETY SUMMARY 
 
 
 
 
 
Safety Notices on Fluorescence Spectrophotometer   
 
Electricity  
 
  Make sure that the fluorescence spectrophotometer is supplied with 
power of 100 V AC, 700 VA or more (50 Hz or 60 Hz).    If the power 
voltage fluctuates or noise is applied to the power line, the 
spectrophotometer may be adversely affected, resulting in any 
trouble in it.   
  Be sure to provide grounding connection together with power line 
connection.    The grounding resistance should be 100 ohms or less.   
Check that the grounding connection is proper.    If the grounding 
connection is improper, the spectrophotometer may become 
vulnerable to external noise or stray voltage may be produced in it, 
resulting in an electric shock hazard.   
  The high voltage circuit is used inside the spectrophotometer.     
To prevent electric shock, do not open covers during operation 
unless otherwise necessary.   
 
 
Source of Heat/Flame   
 
Do not smoke or hold a flame near the fluorescence spectrophotometer.   
 
 
Data Backup   
 
Data stored on the hard disk may become unusable due to a system 
failure, wrong operation, computer virus infection, etc.    To ensure data 
integrity in case of accidental damage to the hard disk, periodically 
make backup copies of hard disk files onto floppy disks.  
To prevent an erroneous operation on the hard disk, always reserve 
approx. 100 MB of free space as a working area for application software.    
						

							 
SAFETY - 4 
 SAFETY SUMMARY 
 
 
 
 
 
Protection against Computer Viruses   
 
If any program/data is damaged suddenly or an unexpected 
operation/screen is encountered, the personal computer is suspected of 
being infected by a computer virus.     
Computer viruses are malicious programs that sneak into personal 
computers to cause misbehavior or damage to data.    And, a program 
designed to offer protection against computer viruses is called a vaccine 
program.  
 
Possible causes of virus infection are:   
  Downloading a virus-laden program through communication.   
  Using a floppy disk or other storage medium infected by a virus.   
 
Note also that once any personal computer is infected by a virus, it may 
spread to other computers via communication or storage medium.   
Never use a program or storage medium that is suspected of 
containing a virus.   
If there is a possibility of virus infection, check for a virus using a 
vaccine program.    Note, however, that some kinds of vaccine programs 
cannot eradicate particular viruses.   So, be sure to make a backup of 
hard disk files beforehand.   
The user is requested to prepare a vaccine program and carry out virus 
removal on his or her own responsibility.   
 
 
Power Interruption   
 
On occurrence of momentary power voltage drop due to power 
interruption or lightning, the personal computer may become faulty or 
the system software, application software or data may be damaged.   
For protection against momentary power voltage drop, it is advisable to 
use an AC uninterruptible power supply unit (stated according to the 
Japanese Electronic Industry Development Association guidelines for 
protection against momentary power voltage drop in personal 
computers).  
  
						

							 
SAFETY - 5 
 SAFETY SUMMARY 
 
 
 
 
 
Turning On/Off Personal Computer   
 
Do not turn off power to the personal computer while the hard disk or 
floppy disk drive is active.    If power to the personal computer is turned 
off while the hard disk or floppy disk is being accessed, the personal 
computer may become faulty or data/software stored in it may be 
damaged.  
Before turning off power to the personal computer, be sure to quit the 
fluorescence spectrophotometer control/data processing program (FL 
Solutions program) first and then take the shut-down procedure using 
the system software.   
  
						

							- i - 
CONTENTS 
 
 
PREFACE 
 
ABOUT THIS MANUAL 
 
IMPORTANT............................................................................................................... IMPORTANT-1 
Precautions on CE Conformity Marking...................... IMPORTANT-1 
1.  Electromagnetic Compatibility Requirement..... IMPORTANT-1 
2. Safety Requirement .......................................... IMPORTANT-1 
Information for Users on WEEE   
(only for EU Countries) ............................................... IMPORTANT-2 
Information on Disposal for Users .............................. IMPORTANT-2 
1.  In the European Union...................................... IMPORTANT-2 
2.  In other Countries outside the EU .................... IMPORTANT-2 
Warranty on Product ................................................... IMPORTANT-3 
Installation, Relocation and After-sale Technical   
Service ........................................................................ IMPORTANT-4 
Technical Seminars and Training for Customers ....... IMPORTANT-5 
Precautions on Disposal of Instrument ....................... IMPORTANT-5 
Other Precautions ....................................................... IMPORTANT-6 
Handling of Chemicals and Samples..................... IMPORTANT-6 
Notice on This Instruction Manual ......................... IMPORTANT-6 
Trademark Acknowledgments ............................... IMPORTANT-6 
 
SAFETY SUMMARY...................................................................................................SAFETY-1 
PRECAUTIONS ON SAFETY ............................................SAFETY-1 
General Safety Guidelines ..........................................SAFETY-1 
Safety Notices on Fluorescence Spectrophotometer .........SAFETY-3 
Electricity........................................................................SAFETY-3 
Source of Heat/Flame....................................................SAFETY-3 
Data Backup ..................................................................SAFETY-3 
Protection against Computer Viruses ............................SAFETY-4 
Power Interruption .........................................................SAFETY-4 
Turning On/Off Personal Computer...............................SAFETY-5 
 
1.    FUNCTIONS AND SCREEN COMPOSITION......................................................................... 1-1 
1.1 Functions .............................................................................. 1-1 
1.2  Installation of FL Solutions Program..................................... 1-5 
1.2.1  Specifications of Personal Computer ...................... 1-5 
1.2.2  Setup of FL Solutions Program ............................... 1-5 
1.2.3  Reinstallation of this Program.................................. 1-9 
1.2.4  Setup of USB Driver ................................................ 1-10  
						

							- ii - 
1.3  Startup of FL Solutions Program .......................................... 1-12 
1.4 Screen Composition ............................................................. 1-16 
1.4.1  Basics of Window Operation ................................... 1-16 
1.4.2  Kinds of Windows .................................................... 1-20 
1.4.3  Data Processing Window ........................................ 1-21 
1.4.4  Explanation of Tool Buttons ..................................... 1-23 
1.5  Functions of Function Keys .................................................. 1-27 
1.6  Explanation of Monitor Window ............................................ 1-28 
1.7  Input of Spectrophotometer Serial No. ................................. 1-30 
1.8 Window Setting..................................................................... 1-31 
1.8.1 Display Tab .............................................................. 1-32 
1.8.2 Fonts Tab................................................................. 1-34 
1.8.3 Processing Tab........................................................ 1-35 
1.8.4 Colors Tab ............................................................... 1-36 
1.8.5 Startup Tab .............................................................. 1-38 
1.8.6  Sensitivity Slits Tab .................................................. 1-39 
1.8.7  Analysis Condition Tab ............................................ 1-40 
1.8.8 Report Tab ............................................................... 1-41 
1.9  Saving a Data File ................................................................ 1-42 
1.10  Photometer Menu Commands ............................................. 1-43 
1.11  Utility Menu Commands ....................................................... 1-44 
1.12  Termination of FL Solutions Program................................... 1-46 
 
2.  WAVELENGTH SCAN............................................................................................................. 2-1 
2.1  Flow of Operation ................................................................. 2-1 
2.2  Creating an Analysis Method ................................................ 2-2 
2.2.1 General Tab ............................................................. 2-3 
2.2.2 Instrument Tab ........................................................ 2-5 
2.2.3 Monitor Tab.............................................................. 2-9 
2.2.4 Processing Tab........................................................ 2-11 
2.2.5 Report Tab ............................................................... 2-14 
2.3  Defining Your Samples ......................................................... 2-16 
2.3.1  Without Using Sample Table ................................... 2-16 
2.3.2  With Sample Table .................................................. 2-17 
2.4 Prescan ................................................................................ 2-21 
2.5 Conducting Measurement .................................................... 2-22 
2.5.1  Without Using Sample Table ................................... 2-22 
2.5.2  With Sample Table .................................................. 2-23 
2.6 Data Printout......................................................................... 2-26 
 
3.  TIME SCAN.............................................................................................................................. 3-1 
3.1  Flow of Operation ................................................................. 3-1 
3.2  Creating an Analysis Method ................................................ 3-2 
3.2.1 General Tab ............................................................. 3-3 
3.2.2 Instrument Tab ........................................................ 3-5  
						

							- iii - 
3.2.3 Monitor Tab.............................................................. 3-8 
3.2.4 Processing Tab........................................................ 3-10 
3.2.5 Report Tab ............................................................... 3-13 
3.3  Defining Your Samples ......................................................... 3-15 
3.3.1  Without Using Sample Table ................................... 3-15 
3.3.2  With Sample Table .................................................. 3-16 
3.4 Conducting Measurement .................................................... 3-20 
3.4.1  Without Using Sample Table ................................... 3-20 
3.4.2  With Sample Table .................................................. 3-21 
3.4.3  Measurement in Stopped Flow Mode ...................... 3-23 
3.5 Data Printout......................................................................... 3-26 
3.6  Calculating the Phosphorescence Life ................................. 3-29 
 
4.  PHOTOMETRY........................................................................................................................ 4-1 
4.1  Flow of Operation ................................................................. 4-1 
4.2  Creating an Analysis Method ................................................ 4-2 
4.2.1 General Tab ............................................................. 4-3 
4.2.2 Quantitation Tab ...................................................... 4-5 
4.2.3 Instrument Tab ........................................................ 4-16 
4.2.4 Standards Tab ......................................................... 4-18 
4.2.5 Monitor Tab.............................................................. 4-20 
4.2.6 Report Tab ............................................................... 4-21 
4.3  Defining Your Samples ......................................................... 4-23 
4.3.1  Without Using Sample Table ................................... 4-23 
4.3.2  With Sample Table .................................................. 4-24 
4.4 Conducting Measurement .................................................... 4-28 
4.4.1 Standards Measurement ......................................... 4-28 
4.4.2 Sample Measurement ............................................. 4-30 
4.4.3  Explanation of Measurement Result Window.......... 4-37 
4.4.4 Re-measurement ..................................................... 4-40 
4.4.5 Statistical Calculation............................................... 4-42 
4.5 Data Printout......................................................................... 4-43 
4.6  Manual Input of Standard Data............................................. 4-45 
4.7  Measurement Using Saved Calibration Curve ..................... 4-46 
 
5.  3-D SCAN................................................................................................................................. 5-1 
5.1  Flow of Operation ................................................................. 5-1 
5.2  Creating an Analysis Method ................................................ 5-2 
5.2.1 General Tab ............................................................. 5-3 
5.2.2 Instrument Tab ........................................................ 5-5 
5.2.3 Monitor Tab.............................................................. 5-8 
5.2.4 Processing Tab........................................................ 5-10 
5.2.5 Report Tab ............................................................... 5-11 
5.3  Defining Your Samples ......................................................... 5-13 
5.4 Conducting Measurement .................................................... 5-14  
						

							- iv - 
5.5  Displaying Data in a Bird’s-Eye View .................................... 5-18 
5.6  Contour Scale Expansion with Mouse .................................. 5-19 
5.7  Auto Scale Display of Data ................................................... 5-21 
5.8  Linking Trace Cursors from Peak Table............................... 5-23 
5.9 Data Printout......................................................................... 5-24 
 
6.  3-D TIME SCAN....................................................................................................................... 6-1 
6.1  Flow of Operation ................................................................. 6-1 
6.2  Creating an Analysis Method ................................................ 6-2 
6.2.1 General Tab ............................................................. 6-3 
6.2.2 Instrument Tab ........................................................ 6-5 
6.2.3 Monitor Tab.............................................................. 6-8 
6.2.4 Processing Tab........................................................ 6-10 
6.2.5 Report Tab ............................................................... 6-11 
6.3  Defining Your Samples ......................................................... 6-13 
6.4 Conducting Measurement .................................................... 6-14 
6.5  Displaying Data in a Bird’s-Eye View .................................... 6-17 
6.6  Contour Scale Expansion with Mouse .................................. 6-18 
6.7  Auto Scale Display of Data ................................................... 6-20 
6.8  Linking Trace Cursors from Peak Table............................... 6-21 
6.9 Data Printout......................................................................... 6-22 
 
7.  DATA PROCESSING............................................................................................................... 7-1 
7.1  Loading of Data .................................................................... 7-1 
7.2 Spectrum Tracing ................................................................. 7-4 
7.3 Scale Change ....................................................................... 7-6 
7.3.1  Change by Using Axes Tab ..................................... 7-6 
7.3.2  Change by Right-clicking Mouse ............................. 7-7 
7.3.3  Change by Using Left Mouse Button ....................... 7-8 
7.4  Change of Graph Axis .......................................................... 7-9 
7.5  Change of Spectral Line ....................................................... 7-10 
7.6  Detection of Spectral Peaks ................................................. 7-12 
7.7  Indication of Peak Information on Spectrum ........................ 7-14 
7.8  Smoothing of Spectrum ........................................................ 7-16 
7.9  Derivative Operation on Spectrum ....................................... 7-17 
7.10  Overlaid Display of Spectra .................................................. 7-18 
7.10.1  Insertion from Window............................................. 7-18 
7.10.2  Insertion from File .................................................... 7-20 
7.10.3  Direct Drag-and-Drop Operation ............................. 7-22 
7.10.4  Selection of Spectra from Overlay Window ............. 7-23 
7.11  Arithmetic Operation between Spectra ................................. 7-24 
7.12 Area Calculation ................................................................... 7-27 
7.12.1  Setting of Calculation Range ................................... 7-27 
7.12.2  Printing of Calculation Result................................... 7-30 
7.12.3 Scale Expansion ...................................................... 7-32  
						

							- v - 
7.13  Transfer of Data to Microsoft(R) Excel ................................. 7-33 
7.14  Pasting of Spectrum to Microsoft(R) Word........................... 7-35 
7.15  Pasting of Spectrum to Microsoft(R) Excel........................... 7-36 
7.16  Conversion of Data into ASCII Text File ............................... 7-37 
7.17  Conversion of Overlaid Spectra into ASCII File.................... 7-39 
7.18  Renaming Your Sample ....................................................... 7-42 
7.19  Printing of Wavelength and    Photometric Values on   
Display .................................................................................. 7-43 
7.19.1  Reading and Printing of Presently Displayed   
Wavelength and Photometric Values ...................... 7-43 
7.19.2  Reading and Printing of Wavelength and   
Photometric Values from Data Processing   
Window .................................................................... 7-46 
7.20  Reference to Spectrum Measured in Photometry Mode ...... 7-50 
 
8.    MORE CONVENIENT OPERATION (APPLICATION)............................................................ 8-1 
8.1  Always Starting with Same Analysis Method ........................ 8-1 
8.2  Online/Offline Setting of Spectrophotometer ........................ 8-3 
8.3  Check of Data File Information ............................................. 8-4 
8.3.1  Check of Information on Saved Data....................... 8-4 
8.3.2  Check of Information on Displayed Data ................. 8-6 
8.4  Saving of Analysis Method ................................................... 8-8 
8.5 Event Log ............................................................................. 8-10 
8.6  Check of Program Version ................................................... 8-12 
8.7  Change of Window Display for Multiple Spectra .................. 8-13 
8.8  Check of Photometric Value at Specified Wavelength ......... 8-16 
8.9  Collective Printing of Files .................................................... 8-17 
8.10  Collective Conversion of Files .............................................. 8-19 
8.11  File Loading with Preview ..................................................... 8-23 
8.12  Enlargement of Values on Monitor ....................................... 8-25 
8.13  Display of Monitor Window at Forefront ............................... 8-26 
 
9.    MEASUREMENT USING OPTIONAL ACCESSORIES........................................................... 9-1 
9.1  Use of Sample Sipper........................................................... 9-1 
9.1.1  When Not Using Sample Table ............................... 9-1 
9.1.2  When Using Sample Table...................................... 9-5 
9.1.3  In Wavelength Scan or Time Scan Mode ................ 9-9 
9.2  Use of AS-3000 Intelligent Autosampler............................... 9-10 
9.3 Analog Output....................................................................... 9-12 
 
 
APPENDIX...................................................................................................................................... A-1 
APPENDIX A  DETAILS OF QUANTIFICATION.......................... A-1 
APPENDIX B  DETAILS OF RATE ANALYSIS FUNCTION ........ A-6