Hitachi F7000 Instruction Manual
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![Page 374
A - 61
H.2 Time Scan
The data sampling interval is set as follows according to the scan time.
[Data mode = Fluorescence/Luminescence/Phosphorescence/
Phosphorescence Life Time]
Sampling Interval [ms]
Phosphorescence
Mode/Chopping Speed Scan Time
[s] Fluorescence/Luminescence/
Phosphorescence
Life Time Mode
40 Hz 20 Hz 10 Hz
0.1 ~ 1 1 50 100 200
~ 3 1 50 100 200
~ 7 2 50 100 200
~ 18 5 50 100 200
~ 36 10 50 100 200
~ 72 20 50 100 200
~ 180 50 50 100...](http://img.usermanuals.tech/thumb/33/65127/w64_hitachi-f7000-instruction-manual-1492072981_d-373.png "Page 374
A - 61
H.2 Time Scan
The data sampling interval is set as follows according to the scan time.
[Data mode = Fluorescence/Luminescence/Phosphorescence/
Phosphorescence Life Time]
Sampling Interval [ms]
Phosphorescence
Mode/Chopping Speed Scan Time
[s] Fluorescence/Luminescence/
Phosphorescence
Life Time Mode
40 Hz 20 Hz 10 Hz
0.1 ~ 1 1 50 100 200
~ 3 1 50 100 200
~ 7 2 50 100 200
~ 18 5 50 100 200
~ 36 10 50 100 200
~ 72 20 50 100 200
~ 180 50 50 100...")
![Page 375
A - 62
H.3 3-D Time Scan
The data sampling interval is set as follows according to the scan speed.
[Data mode = Fluorescence/Luminescence]
Scan Speed (nm/min) Sampling Interval (nm)
60000 2.0
30000 1.0
12000 1.0
2400 1.0
1200 1.0
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 40 Hz]
Scan Speed (nm/min) Sampling Interval (nm)
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 20/10 Hz]
Scan Speed (nm/min) Sampling Interval (nm)...](http://img.usermanuals.tech/thumb/33/65127/w64_hitachi-f7000-instruction-manual-1492072981_d-374.png "Page 375
A - 62
H.3 3-D Time Scan
The data sampling interval is set as follows according to the scan speed.
[Data mode = Fluorescence/Luminescence]
Scan Speed (nm/min) Sampling Interval (nm)
60000 2.0
30000 1.0
12000 1.0
2400 1.0
1200 1.0
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 40 Hz]
Scan Speed (nm/min) Sampling Interval (nm)
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 20/10 Hz]
Scan Speed (nm/min) Sampling Interval (nm)...")
INDEX - 3 I Include calibration curve..................................................................................................................... 4-22 Info tab.................................................................................................................................................. 8-6 Instrument Response .......................................................................................... 1-44, 2-7, 5-7, 6-7, A-30 Instrument tab............................................................................................... 2-5, 3-5, 4-16, 5-5, 6-5, A-41 Instrument toolbar............................................................................................................................... 1-23 Integration method............................................................................................................2-13, 7-12, A-10 Integration time ................................................................................................................................... 4-17 Interface................................................................................................................................................ 1-5 Interrupt (interrupting measurement).................................................................................... 4-30, 9-1, 9-8 Intracellular cation scan ...................................................................................................................... 1-32 J JCAMP-DX file.................................................................................................................... 1-3, 7-37, 8-21 K K-Factor ..................................................................................................................................... 3-12, 4-11 L Lamp Off ............................................................................................................................................. 1-45 Lamp Usage ....................................................................................................................................... 1-44 Least squares ......................................................................................................... 1-1, A-1, A-2, A-6, A-7 Lines/Points tab .................................................................................................................................. 7-10 Load ..........................................................................................................................2-4, 3-4, 4-4, 5-4, 6-4 Lower concentration limit/Upper concentration limit........................................................................... 4-15 Luminescence ...................................................................................................................... 2-5, 3-5, 4-16 M Main memory ........................................................................................................................................ 1-5 Manual calibration .............................................................................................................................. 4-15 Measurement mode........................................................................................ 1-1, 2-3, 3-3, 4-3, 5-3, 6—3 Measurement toolbar.......................................................................................................... 1-23, 1-24, 5-2 Median smoothing .............................................................................................................................. A-15 Menu bar ............................................................................................................................................ 1-16 Monitor tab..............................................................2-9, 3-8, 4-20, 5-8, 6-8, A-43, A-46, A-53, A-55, A-58 N Number of wavelengths...................................................................................................................... 4-14
INDEX - 4 O OS......................................................................................................................................................... 1-5 One wavelength calculation ................................................................................................................. 4-6 Online/offline......................................................................................................................................... 8-3 Overlay ...................................................................................................................................... 8-13, 8-14 P PMT Voltage 0-1000V ............................................................................................2-7, 3-6, 4-17, 5-6, 6-6 PMT voltage ...........................................................................................................2-7, 3-6, 4-17, 5-6, 6-6 Peak area .................................................................................................................................... 4-7, 7-50 Peak detection/finding ......................................................................................1-3, 2-13, 3-11, 5-10, 7-12 Peak height.................................................................................................................................. 2-24, 4-5 Peak table............................................................................................. 1-21, 1-35, 2-24, 3-14, 5-16, 5-23 Phosphorescence attenuation curve .................................................................................................. 3-29 Phosphorescence life ............................................................................................. 1-26, 3-29, A-45, A-61 Photometry .................................................................................................................................. 1-1, 1-22 Photomultiplier tube ................................................................................................2-7, 3-6, 4-17, 5-6, 6-6 Point Polarization time scan ........................................................................................................................ 1-33 Pre-scan .................................................................................................................................... 1-43, 2-21 Prediction measurement time............................................................................................................... 6-7 Preview ............................................................................................................................................... 8-23 Print Preview ........................................................................................................... 2-26, 3-26, 4-43, 5-24 Processing choices............................................................................................................................. 2-11 Processing tab ............................................................................ 1-35, 2-11, 3-10, 5-10, 6-10, A-43, A-64 Processing toolbar ..................................................................................................................... 1-23, 1-26 Q Quantitation tab .................................................................................................................................... 4-5 Quantitation type .................................................................................................................................. 4-5 R Rate calculation (kinetics) ........................................................................................................... 1-1, 3-12 Ratio ................................................................................................................................................... 4-11 Regression calibration curve ................................................................................................................ 1-1 Remeasurement ............................................................................................. 4-40, 9-1, 9-2, 9-6, 9-7, 9-8 Repetition ...................................................................................................................................... 2-8, 3-7 Report generator .................................................................................................................................. 1-4 Report tab ............................................. 1-41, 2-14, 3-13, 4-21, 5-11, 6-11, A-44, A-47, A-53, A-56, A-59 Response ........................................................................................................................ 2-7, 3-6, 5-7, 6-7
INDEX - 5 S Sample data .............................................................................................................................. 4-22, 4-40 Sample sipper................................................................................................................................ 9-1, 9-5 Sample table........................................................................................................................... 2-3, 3-3, 4-3 Save ................................................................................................................................ 2-4, 3-4, 4-4, 5-4 Scale Y Axis Down ............................................................................................................................. 8-13 Scale Y Axis Up .................................................................................................................................. 8-13 Scan speed.................................................................................................................................... 2-6, 5-6 Scan tab ............................................................................................................................................... 8-6 Segmented .................................................................................................................................. 4-13, A-3 Self-diagnosis ..................................................................................................................................... 1-14 Sensitivity check ................................................................................................................................. 1-44 Sensitivity Slits.................................................................................................................................... 1-39 Serial No. ............................................................................................................................................ 1-30 Set Wavelength .................................................................................................................................. 4-17 Show gridlines with increment ............................................................................................................ 1-32 Show trace display ............................................................................................................................. 1-32 Shutter ..................................................................................................................1-24, 1-43, 2-7, 3-6, 5-7 Slope .................................................................................................................................................. 3-22 Smoothing ................................................................................................................ 1-3, 7-16, A-10, A-13 Spectral arithmetic .............................................................................................................................. 7-24 Spectral calculation ..................................................................................................................... 1-2, 7-24 Spectrophotometer menu ................................................................................................................... 1-43 Spectrum toolbar ....................................................................................................................... 1-23, 1-25 Spectrum tracing .................................................................................................................................. 1-3 Spin button ......................................................................................................................................... 1-18 Stack.......................................................................................................................................... 8-13, 8-14 Standard deviation..................................................................................................... 1-2, 4-17, 4-38, 4-42 Standard toolbar ........................................................................................................................ 1-23, 1-24 Standards ........................................................................................................................... 4-2, 4-18, 4-28 Standards tab ..................................................................................................................................... 4-18 Startup tab .......................................................................................................................................... 1-38 Statistical calculation ................................................................................................................... 1-2, 4-42 Stopped flow ................................................................................................................................ 3-7, 3-23 Synchronous (spectrum) ............................................................................................................. 2-5, 2-21 T Tab ..................................................................................................................................................... 1-18 Three wavelength calculation ............................................................................................................... 4-7 Threshold........................................................................................................2-13, 3-11, 5-10, 6-10, 7-13 Tile ............................................................................................................................................. 8-13, 8-15 Time scan ...................................................................................................................................... 1-1, 3-1 Time scan spectrum .................................................................................................................. 6-12, 6-15
INDEX - 6 Tool button................................................................................................................................. 1-17, 1-23 Toolbar ...................................................................................................................................... 1-17, 1-24 U USB cable........................................................................................................................................... 1-12 USB driver .......................................................................................................................................... 1-10 User notes .......................................................................................................................................... 8-10 Utility ................................................................................................................................................... 1-44 V Version ............................................................................................................................................... 8-12 View menu .......................................................................................................................................... 8-13 W Wave number ....................................................................................................................................... 7-9 Wavelength drive ................................................................................................................................ 8-16 Wavelength mode............................................................................................................................... 4-16 Wavelength scan ......................................................................................................... 1-1, 1-21, 1-32, 2-1 Windows metafile ............................................................................................................................... 7-37 Z Zero-order light ................................................................................................................................... 8-16 Zero-point adjustment (Zero Adjust).......................................................................................... 1-25, 1-43