Hitachi F7000 Instruction Manual

							4.6 
4 - 45 
4.6    Manual Input of Standard Data   
 
The standard data can be entered manually.    This function is usable 
after measurement of standards.    Either select Standard Data from the 
Edit menu on the monitor window or click the 
 button.  A window 
as shown in Fig. 4-47 will appear.    So enter values in the Data column.   
After entry, click the OK or Update button.    Then, the calibration curve 
is recalculated and displayed.   
 
 
 
Fig. 4-47 
 
  
						

							4.7    Measurement Using Saved Calibration Curve 
4 - 46 
4.7    Measurement Using Saved Calibration Curve   
 
Call a calibration curve in the following way.   
 
(1)  Set measurement mode in Photometry.   
 
(2)  From the File menu, select the calibration curve load command.   
The following message is reported in a dialog box.     
This message does not appear immediately after turning on 
power supply or when measurement mode is other than 
Photometry.  
Click the OK button.   
 
 
 
Fig. 4-48 
 
 
(3)  A window as shown below will appear.    Select the file of the 
calibration curve to be used.   
After file selection, click the Open button.   
 
 
 
Fig. 4-49 
  
						

							4.7 
4 - 47 
(4)  The window in Fig. 4-50 is now displayed.    The sample data 
section is blank.     
 
 
 
Fig. 4-50 
 
 
 (5)  Click the 
  button, and the window in Fig. 4-51 will appear.   
Enter a sample name and comment.   
Click the OK button.   
 
 
 
Fig. 4-51  Sample data 
section  
						

							4.7    Measurement Using Saved Calibration Curve 
4 - 48 
(6)  Set a sample to be measured.    Click the  button.   
(Or select the Scan command from the Spectrophotometer 
menu.)  
 
(7)  The following message box appears.   
Click the OK button.   
 
 
 
Fig. 4-52 
 
 
(8) The Sample button is now active.    Click this button.   
Measurement starts.   
 
 
 
Fig. 4-53 
 
 
(9)  After measurement, click the End(F9) button for termination.    
						

							5.1 
5 - 1 
5.  3-D SCAN  
 
5.1  Flow of Operation  
 
This section explains the parameter setting and operating method for 3-
D scan.    Measurement proceeds along the following flow.   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Fig. 5-1    Operational Flow (3-D scan)   Click 
   button and set your analytical conditions. 
 
 • Scan range 
• Data mode 
•  Scan speed, etc. 
Click 
 button. 
 • Sample name 
• Auto File 
•  File name, etc. 
Set a sample. 
Click 
  button to execute wavelength scan. 
• Spectrum tracing 
• Peak detection 
• Spectral calculation 
Click 
  button.    Measurement result will be displayed 
in a report format. 
 
Start 
Method setting 
(See 5.2.) 
Sample name/ 
comment input 
(See 5.3.) 
Measurement 
(See 5.4.) 
Data processing 
(See Section 7.) 
Result output in report 
(See 5.9.) 
End  
						

							5.2    Creating an Analysis Method 
5 - 2 
5.2    Creating an Analysis Method   
 
Set the analytical conditions (method) prior to measurement.   
 
(1) Click the 
  button on the Measurement toolbar.    The window 
in Fig. 5-2 appears.   
(Or select the Method command from the Edit menu.)     
 
 
 
Fig. 5-2  Analysis Method  
 
 
(2)  The following tabs are provided on the Analysis Method window 
for the 3-D scan mode.    These tabs are detailed on the 
subsequent pages.   
• General  
• Instrument  
• Monitor  
• Processing  
• Report  
 
  
						

							5.2.1 
5 - 3 
The parameters shown in Fig. 5-3 are provided at the General tab.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 5-3  General Tab 
 
 
1 Measurement (mode)   
A measurement mode is selectable.    Here, select “3-D Scan.”   
For Wavelength scan, Time scan, Photometry and 3-D Time scan, 
refer to Sections 2, 3, 4 and 6, respectively.   
2 Operator  
Enter an operator name.   
3 Instrument  
The name of the connected instrument is indicated.   
4 Sampling 
- Standard   
Indicates that samples are to be exchanged manually.     
5 Comments  
Enter a description or notes on measuring conditions.   
5.2.1 General Tab 
 
1 
2 
3 
4 
5 
6 
7 
8  
						

							5.2    Creating an Analysis Method 
5 - 4 
6 Load  
Click this button to read out saved analytical conditions.     
Upon clicking, the file opening window appears.    So, select an 
analytical condition file.   
7 Save  
When you click this button after setting the parameters, the 
analytical conditions can be saved by overwriting.    In case of a 
new analysis method, clicking this button displays the “Save As” 
window.    You must enter a file name for saving in this window.   
8 Save As   
Click this button for renaming the set conditions for saving.     
The “Save As” window appears, so enter a file name.   
 
  
						

							5.2.2 
5 - 5 
The window shown below is based on that 3-D Scan is specified for 
Measurement at the General tab.    When you click the Instrument tab, 
the window in Fig. 5-4 appears.   
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 5-4  Instrument Tab 
 
 
1 Data mode   
Select a data mode.    The Phosphorescence mode is for acquiring 
only phosphorescence after extinction of excitation beam while 
turning a chopper on the excitation side.   
2 Chopping speed   
Select a chopping speed.    Setting is allowed when 
Phosphorescence is selected for Data mode.     
The Phosphorescence mode is for acquiring only phosphorescence 
after extinction of excitation beam while turning a chopper on the 
excitation side.    An increase in chopping speed makes the 
chopper turns faster.   
3 EX Start WL   
This is the start wavelength on the excitation side in wavelength 
scan.    Enter the shorter wavelength side of measuring wavelength 
range.  
5.2.2 Instrument 
Tab 
 
3 
4 
5 
6 
7 
17 
2 
1 
8 
9 
12
13
14
15
16
11
10
18  
						

							5.2    Creating an Analysis Method 
5 - 6 
4 EX End WL   
This is the end wavelength on the excitation side in wavelength 
scan.    Enter the longer wavelength side of measuring wavelength 
range.  
5 EX Sampling interval   
In the 3-dimensional scan mode, emission spectrum measurement 
is repeated while shifting the excitation wavelength.    Therefore, a 
shorter sampling interval on the excitation side will result in a longer 
measurement time.   
6 EM Start WL   
This is the start wavelength on the emission side in wavelength 
scan.    Enter the shorter wavelength side of measuring wavelength 
range.  
7 EM End WL   
This is the end wavelength on the emission side in wavelength 
scan.    Enter the longer wavelength side of measuring wavelength 
range.  
8 EM Sampling interval   
In the 3-dimensional scan mode, emission spectrum measurement 
is repeated while shifting the excitation wavelength.    Therefore, a 
shorter sampling interval on the emission side will result in a longer 
measurement time.   
9 Scan speed   
Set a wavelength scan speed.   
10 EX Slit   
Select a slit width on the excitation side.   
11 EM Slit   
Select a slit width on the emission side.   
12 PMT Voltage   
A function for controlling the voltage of the photomultiplier detector 
(photomultiplier tube).    As a reference, changing from 400 V to 
700 V increases the data value about two digits and changing from 
700 V to 950 V increases it about 1 digit.   
When a check mark is put for PMT Voltage 0-1000V, the 
photomultiplier voltage is settable within a range from 0 to 1000 V.   
13 PMT Voltage 0-1000V   
When a check mark is put in this box, the photomultiplier voltage is 
settable within a range from 0 to 1000 V.