Hitachi F7000 Instruction Manual

							4.2.2 
4 - 15 
5 Manual calibration   
Put a check mark in this box for creating a calibration curve using 
factors.    Enter factors in response to the selected calibration type.     
6 Force curve through zero   
By putting a check mark in this box, a calibration curve is created 
so that its factor A
0 takes 0 automatically.     
7 Digit after decimal point   
Set the number of digits below decimal point for indicating the 
calculated concentration of the measured sample.     
The concentration value is rounded off to the set number of digits.   
8 Lower concentration limit/Upper concentration limit   
This is used to set a normal value range for the concentration of 
the measured sample.    If the concentration is higher than the 
upper limit set here, “HIGH” will appear in the Conc column, and if 
lower than the lower limit set here, “LOW” will appear in the Conc 
column.  
 
 
 
Fig. 4-14    (measurement example with upper limit set at 5) 
 
  
						

							4.2    Creating an Analysis Method 
4 - 16 
When you click the Instrument tab, the window in Fig. 4-15 appears.   
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 4-15  Instrument Tab 
 
 
1 Data mode   
Select one of the three data modes.    The Luminescence mode is 
for measuring luminescence in the biological/chemical field.     
In this mode, only the sample side signal is acquired without 
performing ratio photometry.     
2 Chopping speed   
Select a chopping speed.    Setting is allowed when 
Phosphorescence is selected for Data mode.     
The Phosphorescence mode is for acquiring only phosphorescence 
after extinction of excitation beam while turning a chopper on the 
excitation side.    An increase in chopping speed makes the 
chopper turns faster.   
3 Wavelength mode   
Selects a wavelength setting for creation of a calibration curve.   
4.2.3 Instrument 
Tab 
 
1 
2 
3 
10
11
12
8
7
6
13
4 
9
5  
						

							4.2.3 
4 - 17 
4 Fixed WL 
When ‘EX WL Fixed’ is selected in Wavelength mode, set the fixed 
wavelength on the excitation side.   
When ‘EM WL Fixed’ is selected in Wavelength mode, set the fixed 
wavelength on the emission side.   
5 WL (1 to 6)   
Make your entry for the number of wavelengths specified at the 
Quantitation tab.   
6 EX Slit   
Select a slit width on the excitation side.   
7 EM Slit   
Select a slit width on the emission side.   
8 PMT Voltage   
A function for controlling the voltage of the photomultiplier detector 
(photomultiplier tube).    As a reference, changing from 400 V to 
700 V increases the data value about two digits and changing from 
700 V to 950 V increases it about 1 digit.   
When a check mark is put for PMT Voltage 0-1000V, setting is 
allowed within a range from 0 to 1,000 V.   
9 PMT Voltage 0-1000V   
When a check mark is put in this box, the photomultiplier voltage is 
settable within a range from 0 to 1000 V.   
10 Auto statistic cal. number   
Calculation is made for mean value, SD (standard deviation) and 
CV (coefficient of variation).    Set the number of samples for this 
purpose.  
11 Replicates  
Set the number of measurements to be repeated.     
12 Integration time   
A function for obtaining data averaged over the specified time for 
acquisition of stabilized data.   
13 Delay  
After pressing the Measure button, measurement is started 
following the delay time set here.    It is used for stabilization after 
setting an unknown sample, etc.    In repeat measurement, it is the 
time until the start of the first measurement.    The delay time is 
invalid for the second and subsequent measurements.   
 
  
						

							4.2    Creating an Analysis Method 
4 - 18 
Clicking the Standards tab opens the window shown in Fig. 4-16.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 4-16  Standards Tab 
 
 
1 Number of Samples   
For generating a sample table newly, enter the number of samples 
here, and then click the Update button.   
2 Standard Table   
This table gives a list of standards for sample measurement or 
calibration curve preparation.    The table contains the items listed 
below.  
NOTE:  The size of the standard table columns (cells) is changeable.   
Bring the cursor to a boundary line between the left and right 
table headers, and it changes into one shown below.     
 
Now click the mouse button on the line and drag it to the left or 
right to obtain a suitable column size.   
 
4.2.4 Standards Tab 
 
1 
2 
4
3
5 
						

							4.2.4 
4 - 19 
⎯ No.   
This is the number given to each sample.   
⎯ Sample   
Enter the name of each sample.     
⎯ Comment   
Enter comments on each sample.   
⎯ Concentration   
Enter the concentration of each sample.   
 
3 Update  
When clicking this button, a confirmation message appears.   
 
 
 
 
Clicking Yes will clear the presently entered sample names and 
comments, and table rows are formed by the entered number of 
samples.  
For adding a row below the final one in the sample list, click the 
Insert button.   
4 Insert  
When you click the Insert button, data will be added below the final 
row in the standard list.   
5 Delete  
Click the column of the sample No. to be deleted, and it becomes 
active.  Then, click the Delete button, and the relevant sample 
data will be deleted.   
 
  
						

							4.2    Creating an Analysis Method 
4 - 20 
When selecting the Monitor tab, the window in Fig. 4-17 appears.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 4-17  Monitor Tab 
 
 
Assign the following parameters.   
 
1 Y-Axis Max (maximum)   
Enter a maximum value for Y-axis on the monitor window.   
2 Y-Axis Min (minimum)   
Enter a minimum value for Y-axis on the monitor window.   
3 Open data processing window after data acquisition   
Select whether or not to conduct data processing after sample 
measurement.    When selected (check mark is applied), an icon 
for this function (as shown in 
) is displayed.   
Opening the saved file allows data processing such as peak 
detection.    When this function is not selected, data processing can 
be achieved by reading out the file via [File]-[Open].   
4 Print report after data acquisition   
Select whether or not to print a report after sample measurement.   
When selected (check mark is applied), printing will be made 
automatically after measurement.    The items set at the Report tab 
will be printed.   
 
4.2.5 Monitor Tab 
  
						

							4.2.6 
4 - 21 
Select the Report tab, and the window in Fig. 4-18 will appear.   
 
 
 
Fig. 4-18  Report Tab  
 
 
On this window, select the items to be contained in an analysis report.   
 
1 Output  
• Print Report   
A report will be printed.    For printing method, refer to 4.5.   
• Use Microsoft(R) Excel   
Data will be transferred to Microsoft(R) Excel.    For transferring 
method, refer to 7.13.   
• Use print generator sheet   
Select this method for using the report generator (option).     
For usage, refer to the instruction manual of the report 
generator.  
4.2.6 Report Tab 
 
3 
2 
1  
						

							4.2    Creating an Analysis Method 
4 - 22 
2 Print items (printable/transferable items) 
• Include date   
• Include method   
• Include calibration curve   
• Include standards data   
• Include calibration data   
• Include sample data   
3 Printer Font   
Click the Select button for changing the front to be used in the 
report.  
  
						

							4.3.1 
4 - 23 
4.3  Defining Your Samples  
 
When “Use sample table” is not selected in Analysis Method, a sample 
must be defined in the following procedure before measurement.   
 
(1) Click the 
  button.    The window in Fig. 4-19 will appear.   
 
 
 
Fig. 4-19 
 
 
(2)  Make entries for Sample name and Comment.   
Maximum number of enterable characters (half-size)   
Sample name :  40 characters   
Comment : 255 characters  
 
For automatic saving, put a check mark in the “Auto File” box to 
specify a file name.    First click the Select button, and then 
specify a saving location and enter a file name.   
When you put a check mark at the head of “Auto Text file” and 
“Auto Meta file” after turning on “Auto File,” photometry data can 
be saved automatically in the following file formats as soon as this 
data is automatically saved.   
•  ASCII text file (*.txt)   
•  Graph meta file (*.wmf)   
The data will be saved in the file name entered for “File name.”   
 
(3)  After completion of setting, click OK.  
4.3.1 Without Using 
Sample Table 
  
						

							4.3  Defining Your Samples 
4 - 24 
When “Use sample table” is selected in Analysis Method, samples must 
be defined in the following procedure before measurement.   
Open the monitor window and click the 
 button.  
When the Sample Table window in Fig. 4-20 appears, enter the sample 
name and comment for each sample.   
 
1 For creating a sample table newly, make entry for Number of 
Samples and click the Update button.    For instance, if there are 
five samples, enter “5” for Number of Samples and click the 
Update button.   
 
 
 
Fig. 4-20  Sample Table  
 
 
2 For adding a row at the end of sample table, click the Insert button.   
3 To delete an unnecessary row, click its row No. to make it active as 
shown in Fig. 4-21.    Then, click the Delete button.   
4.3.2 With Sample 
Table 
 
2
1