Hitachi F7000 Instruction Manual
Have a look at the manual Hitachi F7000 Instruction Manual online for free. It’s possible to download the document as PDF or print. UserManuals.tech offer 913 Hitachi manuals and user’s guides for free. Share the user manual or guide on Facebook, Twitter or Google+.
A - 48 G.3.1A Photometry: Quantification Tab (Quantification type: Wavelength) Parameter Name Setting Range Quantification type Wavelength, Peak area, Peak height, Derivative, Ratio Calibration type None, 1st order, 2nd order, 3rd order, Segmented Number of wavelengths Calibration Type Setting Range None 1 to 6 1st order 2nd order 3rd order Segmented 1 to 3 Concentration unit Up to 8 characters Manual calibration ON, OFF ON: Factors are specifiable. Factor Setting Range A0 to A3 -100000 to 100000 Force curve through zero ON, OFF Digit after decimal point 0 to 3 Lower concentration limit 0 to 1e+009 Upper concentration limit 0 to 1e+009 G.3.1B Photometry: Quantification Tab (Quantification type: Peak area, Peak height) Parameter Name Setting Range Quantification type Wavelength, Peak area, Peak height, Derivative, Ratio Calibration type None, 1st order, 2nd order, 3rd order, Segmented Peak apex Wavelength range on Instrument tab page ± 0.1 to 700.0 nm Concentration unit Up to 8 characters Manual calibration ON, OFF ON: Factors are specifiable. Factor Setting Range A0 to A3 -100000 to 100000 Force curve through zero ON, OFF Digit after decimal point 0 to 3 Lower concentration limit 0 to 1e+009 Upper concentration limit 0 to 1e+009 Integration method Rectangular, Trapezoid, Romberg Threshold 0.001 to 1000 Sensitivity 1, 2, 4, 8
A - 49 G.3.1C Photometry: Quantification Tab (Quantification type: Derivative) Parameter Name Setting Range Quantification type Wavelength, Peak area, Peak height, Derivative, Ratio Calibration type None, 1st order, 2nd order, 3rd order, Segmented Peak apex Wavelength range on Instrument tab page Concentration unit Up to 8 characters Manual calibration ON, OFF ON: Factors are specifiable. Factor Setting Range A0 to A3 -100000 to 100000 Force curve through zero ON, OFF Digit after decimal point 0 to 3 Lower concentration limit 0 to 1e+009 (1000000000) Upper concentration limit 0 to 1e+009 (1000000000) Derivative order 2, 4 Smoothing order 2 to 4 Number of points 5 to 101 G.3.1D Photometry: Quantification Tab (Quantification type: Ratio) Parameter Name Setting Range Quantification type Wavelength, Peak area, Peak height, Derivative, Ratio Subtract background [Wavelength 3] ON, OFF K-Factor 0 K-Factor 1 K-Factor 2 -9999 to -0.001, 0.001 to 9999 Operand + (plus), - (minus), * (times)
A - 50 G.3.2A Quantification: Instrument Tab (Quantification type: Wavelength, Ratio) Parameter Name Setting Range Data mode Fluorescence, Luminescence, Phosphorescence Chopping speed 10 Hz, 20 Hz, 40 Hz Wavelength mode EX WL Fixed, EM WL Fixed, Both WL Fixed Fixed WL Input Range: 0, 200 to 900 nm WL 1 to WL 6 Input Range: 0, 200 to 900 nm EX Slit (unit: nm) 1.0 2.5 5.0 10.0 20.0 EM Slit (unit: nm) 1.0 2.5 5.0 10.0 20.0 PMT Voltage Setting range: 250, 400, 700, 950 V 0 to 1000 V when “PMT Voltage 0-1000V” is turned ON (checked). PMT Voltage 0 - 1000 V ON, OFF Auto statistic calc. number 2 to 4000 Replicates 1 to 20 Integration time 0.1 to 10.0 s Delay 0 to 9999 s
A - 51
G.3.2B Photometry: Instrument Tab (Quantification type: Peak area, Peak
height, Derivative)
Parameter Name Setting Range
Scan mode Excitation, Emission, Synchronous
Data mode Fluorescence, Luminescence, Phosphorescence
Chopping speed 10 Hz, 20 Hz, 40 Hz
EX WL
Scan Mode Input Range
Excitation 0, 200 to 900 nm
Synchronous 200 to 890 nm
EX Start WL Input Range: 200 to 890 nm
EX End WL Input Range: 210 to 900 nm
EM WL Input Range: 0, 200 to 900 nm
EM Start WL Input Range: 200 to 890 nm
EM End WL Input Range: 210 to 900 nm
Scan speed (unit: nm/min) 60000
30000
12000
2400
1200
240
60
30
When Synchronous is specified for scan mode,
1200 nm/min or less is indicated.
When Phosphorescence is specified for data mode, select
from the following. Chopping speed is in parentheses.
(40 Hz) (20 Hz/10 Hz)
240
60
30 60
30
Delay 0 to 9999 s
EX Slit (unit: nm) 1.0
2.5
5.0
10.0
20.0
EM Slit (unit: nm) 1.0
2.5
5.0
10.0
20.0
PMT Voltage Setting range: 250, 400, 700, 950 V
0 to 1000 V when “PMT Voltage 0-1000V” is turned ON
(checked).
PMT Voltage 0-1000V ON, OFF
A - 52 (cont’d) Parameter Name Setting Range Response (unit: s) 0.002 0.004 0.01 0.05 0.1 0.5 2.0 4.0 8.0 Auto Corrected spectra ON, OFF Shutter control ON, OFF Replicates 1 to 20 Auto statistic calc. number 2 to 4000
A - 53 G.3.3 Photometry: Standard Tab Parameter Name Setting Range Number of Samples 1 to 20 Sample Up to 40 characters Comment Up to 255 characters Concentration 0 to 1000000000 G.3.4 Photometry: Monitor Tab Parameter Name Setting Range Y Axis: Max. -119999999.9 to 120000000.0 Y Axis: Min. -120000000.0 to 119999999.9 Open data processing window after data acquisition ON, OFF Print report after data acquisition ON, OFF G.3.5 Photometry: Report Tab Parameter Name Setting Range Output Print Report, Use Microsoft(R) Excel, Use print generator sheet Print items: Include date ON, OFF Print items: Include method ON, OFF Print items: Include calibration curve ON, OFF Print items: Include standard data ON, OFF Print items: Include standard comment ON, OFF Print items: Include calibration data ON, OFF Print items: Include sample data ON, OFF Print items: Include sample comment ON, OFF
A - 54
G.4.1 3-D Scan: Instrument Tab
Parameter Name Setting Range
Data mode Fluorescence, Phosphorescence
Chopping speed 10 Hz, 20 Hz, 40 Hz
EX Start WL Input Range: 200 to 850 nm
EX End WL Input Range: 250 to 900 nm
EX Sampling interval Input Range: 1 to 50 nm
EM Start WL Input Range: 200 to 850 nm
EM End WL Input Range: 250 to 900 nm
EM Sampling interval Input Range: 1 to 10 nm
Scan speed (unit: nm/min) 60000
30000
12000
2400
1200
240
60
30
when Synchronous is specified for scan mode,
1200 nm/min or less is indicated.
When Phosphorescence is specified for data mode, select
from the following. Chopping speed is in parentheses.
(40 Hz) (20 Hz/10 Hz)
240
60
30 60
30
EX Slit (unit: nm) 1.0
2.5
5.0
10.0
20.0
EM Slit (unit: nm) 1.0
2.5
5.0
10.0
20.0
PMT Voltage Setting range: 250, 400, 700, 950 V
0 to 1000 V when “PMT Voltage 0-1000V” is turned ON
(checked).
PMT Voltage 0 - 1000 V ON, OFF
Response (unit: s) 0.002
0.004
0.01
0.05
0.1
0.5
2.0
4.0
8.0
Auto
A - 55 (cont’d Parameter Name Setting Range Corrected spectra ON, OFF Shutter control ON, OFF ON : Checked OFF : Unchecked G.4.2 3-D Scan: Monitor Tab Parameter Name Setting Range Y Axis: Max -119999999.9 to 120000000.0 Y Axis: Min -120000000.0 to 119999999.9 Contour interval 0.001 to 1000.000 Open data processing window after data acquisition ON, OFF Print report after data acquisition ON, OFF G.4.3 3-D Scan: Processing Tab Parameter Name Setting Range Peak Finding: Integration method Rectangular Peak Finding: Threshold 0.001 to 1000 Peak Finding: Sensitivity 1, 2, 4, 8
A - 56
G.4.4 3-D Scan: Report Tab
Parameter Name Setting Range
Output Print Report, Use Microsoft(R) Excel, Use print
generator sheet
Orientation Portrait, Landscape
Print items: Include date ON, OFF
Print items: Include instrument ON, OFF
Print items: Include graph ON, OFF
ON: Any of the following items is selectable.
• Contour
• Bird’s-eye view
• Contour and Bird’s-eye view
• Contour and Spectrum
Print items: Include peak table ON, OFF
Print items: Data listing*1 ON, OFF
ON: Setting can be made for excitation and emission
spectra.
Parameter Setting Range
Interval Minimum data interval to
measuring wavelength interval
Start Wavelength range on Instrument
tab page
End Wavelength range on Instrument
tab page
*1: Valid when “Use Microsoft(R) Excel” or “Use print generator sheet” is specified for
Output. Always turned ON when “Use print generator sheet” is specified.
A - 57
G.5.1 3-D Time Scan: Instrument Tab
Parameter Name Setting Range
Scan mode Excitation, Emission, Synchronous
Data mode Fluorescence, Luminescence, Phosphorescence
Chopping speed 10 Hz, 20 Hz, 40 Hz
EX WL
Scan Mode Input Range
Excitation 0, 200 to 900 nm
Synchronous 200 to 890 nm
EX Start WL Input Range: 200 to 890 nm
EX End WL Input Range: 210 to 900 nm
EM WL Input Range: 0, 200 to 900 nm
EM Start WL Input Range: 200 to 890 nm
EM End WL Input Range: 210 to 900 nm
Scan speed (unit: nm/min) 60000
30000
12000
2400
1200
240
60
30
When Synchronous is specified for scan mode,
1200 nm/min or less is indicated.
When Phosphorescence is specified for data mode, select
from the following. Chopping speed is in parentheses.
(40 Hz) (20 Hz/10 Hz)
240
60
30 60
30
EX Slit (unit: nm) 1.0
2.5
5.0
10.0
20.0
EM Slit (unit: nm) 1.0
2.5
5.0
10.0
20.0
PMT Voltage 250, 400, 700, 950 V
0 to 1000 V when “PMT Voltage 0-1000V” is turned ON
(checked).
PMT Voltage 0 - 1000 V ON, OFF