Hitachi F7000 Instruction Manual

							5.2.2 
5 - 7 
14 Response  
When “Auto” is selected, response will be set automatically 
depending on scan speed.    Select “Auto” usually.   
15 Corrected spectra   
A function for determining the spectrum inherent to a sample by 
correcting the photometer wavelength characteristic using the 
saved instrument parameters, following measurement with the 
instrument parameters for photometer control.    If there are no 
instrument parameters for the wavelength range to be measured, 
only the wavelength range that allows correction is scanned.     
And if there is no wavelength area measurable, an error will occur.   
The Instrument Response command is provided in the Utility menu 
for operating this function.   
When this setting is at ON, “Corrected spectra” appears on the 
monitor window.    (See Fig. 1-43 in 1.6.)   
16 Shutter control   
The shutter can automatically be closed in other than measurement 
for slowing down sample deterioration due to the energy of 
excitation beam and opened when measurement starts.     
17 Range of corrected spectra   
Spectrum correction range is indicated.   
18 Prediction measurement time   
The predicted measurement time is indicated.    After change of a 
parameter such as scan speed, click the Update button.   
The predicted value will then be updated.   
 
  
						

							5.2    Creating an Analysis Method 
5 - 8 
When selecting the Monitor tab, the window in Fig. 5-5 appears.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 5-5  Monitor Tab 
 
 
Assign the following parameters.   
 
1 Y-Axis Max (maximum)   
Enter a maximum value for Y-axis on the monitor window.   
2 Y-Axis Min (minimum)   
Enter a minimum value for Y-axis on the monitor window.   
3 Contour interval   
Function for setting intervals of drawing contour lines.     
4 Open data processing window after data acquisition   
Select whether or not to conduct data processing after sample 
measurement.    When selected (check mark is applied), an icon 
(as shown in 
) is displayed for the data 
processing window at the end of measurement.    And by opening 
this icon, data processing such as peak detection is available.   
 
5.2.3 Monitor Tab 
 
2 
1 
4 
5 
3  
						

							5.2.3 
5 - 9 
NOTE:  When the remaining memory capacity of PC in operation is 
very small (system resource is less than 20%), the data 
processing window will not open even if this item is turned ON.   
Either close the presently opened data processing window or 
restart the FL Solutions program.   
 
5 Print report after data acquisition   
Select whether or not to print a report after sample measurement.   
When selected (check mark is applied), printing will be made 
automatically after measurement.    The items set at the Report tab 
will be printed.    When this item is not selected, data processing is 
allowed by reading out a file via [File] - [Open].   
 
  
						

							5.2    Creating an Analysis Method 
5 - 10 
On selection of the Processing tab, the window in Fig. 5-6 will be 
displayed.  
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 5-6  Processing Tab 
 
 
1 Peak Finding:  Integration method  
Select an integration method for the peaks to be displayed in the 
data processing window.   
2 Threshold  
Set a detection limit for the photometric value axis of peak and 
valley.    Peaks or valleys below the setting will not be detected.   
3 Sensitivity  
Select the number of data points in the horizontal axis direction 
(abscissa).    For detecting sharp peaks, select a sensitivity value of 
“1.”    Or select “8” for detecting broad peaks.   
 
 
5.2.4 Processing 
Tab 
1 
3
2 
						

							5.2.5 
5 - 11 
Select the Report tab, and the window in Fig. 5-7 will appear.   
 
 
 
Fig. 5-7  Report Tab 
 
 
1 Output  
• Print Report   
A report will be printed.    For printing method, refer to 5.9.   
• Use Microsoft(R) Excel   
Data will be transferred to Microsoft(R) Excel.    For transferring 
method, refer to 7.13.   
• Use print generator sheet   
Select this method for using the report generator (option).     
For usage, refer to the instruction manual of the report 
generator.  
2 Orientation  
Selectable when Print Report is chosen for Output.   
Select a printing orientation.    Either Portrait or Landscape 
orientation is selectable.    When the Landscape orientation is 
selected, date and method can be printed.   
5.2.5 Report Tab 
 
4 
3 
1 
2 
						

							5.2    Creating an Analysis Method 
5 - 12 
3 Print items (printable/transferable items) 
• Include date   
• Include instrument 
• Include graph   
When you put a check mark for this item, any of the graphs 
listed below is selectable.   
◊ Contour  
◊ Bird’s-eye view  
◊  Contour and Bird’s-eye view   
◊  Contour and Spectrum   
• Include peak table   
• Data listing   
◊ Interval  
◊ Start  
◊ End  
These parameters are settable when “Use Microsoft(R) Excel” 
or “Use print generator sheet” is specified for Output.   
For time scan, make entries for Interval, Start and End.   
For wavelength scan, make entries for Interval, Start and End.   
4 Printer Font   
Click the Select button for changing the font to be used in the 
report.  
  
						

							5.3 
5 - 13 
5.3  Defining Your Samples  
 
A sample must be defined in the following procedure before 
measurement.  
 
(1) Click the 
  (sample name/comment input) button on the 
Measurement toolbar.    The window in Fig. 5-8 will appear.   
 
 
 
Fig. 5-8 
 
 
(2)  Make entries for Sample name and Comment.   
Maximum number of enterable characters (half-size)   
Sample name :  40 characters   
Comment : 255 characters  
 
For automatic saving, put a check mark in the “Auto File” box to 
specify a file name.    First click the Select button, and then 
specify a saving location and enter a file name.   
When you put a check mark at each head of “Auto Text file” and 
“Auto Meta file” after turning on “Auto File,” 3-D scan data can be 
saved automatically in the following file formats as soon as this 
data is automatically saved.   
•  ASCII text file (*.txt)   
•  Graph meta file (*.wmf)   
The data will be saved in the file name entered for “File name.”   
 
(3)  After completion of setting, click OK.   
						

							5.4  Conducting Measurement 
5 - 14 
5.4  Conducting Measurement  
 
(1)  Make sure an analysis method has been set and a sample has 
been defined.    (Refer to “5.2 Creating an Analysis Method” and 
“5.3 Defining Your Samples.”)   
 
(2)  Set a sample in the sample compartment and click the 
 
button on the Measurement toolbar to start measurement.     
(See ①  in Fig. 5-9.)   
On this monitor window, the X and Y axes stand for the EM and 
EX-side wavelengths, respectively.   
 
 
 
Fig. 5-9  Monitor Window 
 
 
(3)  For halting the measurement midway, click the 
 (stop) 
button on the toolbar.    (See ②  in Fig. 5-9.)   
 
(4)  The information to be displayed upon opening the data 
processing window can be selected here by excluding 
unnecessary one from display.   
Select the Options command from the Tools menu and click the 
‘Display’ tab in the Options dialog box.    Then, the window in Fig. 
5-10 appears.    For example, select “Show contour plus EX,EM 
spectrum plus peak table” and click the OK button.   
1
2 
						

							5.4 
5 - 15 
 
 
 
Fig. 5-10 
 
 
(5)  When measurement is completed, the data processing window 
appears as shown in Fig. 5-11.    This is available only when a 
check mark is put for “Open data processing window after data 
acquisition” at the ‘Monitor’ tab of Analysis Method.    (See ④ in 
Fig. 5-5.)   
The measured data will be saved automatically when auto saving 
is selected.   
If auto saving is not selected, select the “Save As” command from 
the File menu and save the data.    Opening the saved data file 
will display the data processing window as shown below.    
						

							5.4  Conducting Measurement 
5 - 16 
 
 
 
 
 
Fig. 5-11    Data Processing Window (3-D scan) 
 
 
① Contour lines  
From these contour lines, excitation and emission spectra can be 
read out.   
As the cursor is moved, the cursor-specified excitation and 
emission spectra are displayed in ② and ③.  
 
② Excitation spectrum  
An excitation spectrum at the cursor position in ① is displayed.  
Wavelength and photometric value can be read by selecting the 
“trace” command from the View menu or clicking the 
 button.   
 
③ Emission spectrum  
An emission spectrum at the cursor position in ① is displayed.  
Wavelength and photometric value can be read by selecting the 
“trace” command from the View menu or clicking the 
 button.   
 
④ Peak table  
Peak wavelength and peak height are indicated.    For setting 
threshold and sensitivity values, click the 
 button.  
 
①Contour lines
②Excitation spectrum 
③Emission spectrum
④ Peak 
table