Hitachi F7000 Instruction Manual
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7.12.1 7 - 27 7.12 Area Calculation An area value is obtainable by integration in the specified range of the presently displayed spectrum. Click the (area calculation) button on the Processing toolbar. A window as in Fig. 7-32 will appear. (Or select the Area command from the Data menu.) Fig. 7-32 The range for area calculation is settable in the following two ways. (1) Setting by Moving Cursor ① Click the Start button. (Refer to ① in Fig. 7-33.) Move the trace cursor to a desired start wavelength on the spectrum (refer to ② in Fig. 7-33), and then click the right button of the mouse. ② Click the End button. (Refer to ③ in Fig. 7-33.) Move the trace cursor to a desired end wavelength on the spectrum (refer to ④ in Fig. 7-33), and then click the right mouse button. 7.12.1 Setting of Calculation Range
7.12 Area Calculation 7 - 28 ③ Click the Calculate button. The result of calculation will be indicated. (Refer to ⑤ in Fig. 7-33.) Fig. 7-33 (2) Setting by Entering Numerical Value ① Enter a desired start wavelength in the Start input box from the keyboard. ② Enter a desired end wavelength in the End input box from the keyboard. ③ Click the Calculate button. The result of calculation will be indicated. 1 3 5 2 4Calculation result
7.12.1 7 - 29 (3) The principle of area calculation is shown below. Fig. 7-34 Principle of Calculation The elements (D 1 to Dn) of a spectrum are subjected to the following calculation for obtaining an area value. S 3 = S1 + S2 = Sa + Sc + Sd = {D 2 + D3 + Dn-2 + Dn-1 + (D1 + Dn)/2∗∆λ S 2 = Sb + Sd = [(D 1 + Dn)∗{∆λ∗ (n – 1)}]/2 = [(D 1 + Dn)∗{section start wavelength (WL1) – section end wavelength (WL 2)}]/2 S 1 = S3 – S2 D 1: Spectral data at section start wavelength D n: Spectral data at section end wavelength ∆λ: Sampling interval In the time scan mode, change as follows. Start wavelength (WL 1) → End time (T2) End wavelength (WL 2) → Start time (T1) D n-1 Dn D2 D1 ∆λ S 1 S2 WL1WL2WL1WL2 S a Sb Sc S d Section end wavelength Specified rangeSection start wavelength
7.12 Area Calculation 7 - 30 The result of area calculation can be printed out. (1) To print a spectrum and the result of area calculation, put a check mark at “Print spectrum.” (Refer to ① in Fig. 7-35.) To avoid printing a spectrum, remove the check mark. (2) Click Print to start printing. (Refer to ② in Fig. 7-35.) Upon execution of printing, the Area window will close. The same window can be called up by clicking the (area calculation) button on the Processing toolbar. Fig. 7-35 Fig. 7-36 Example of Printout (with spectrum record) 7.12.2 Printing of Calculation Result 1 2 3
7.12.2 7 - 31 (3) Upon clicking the OK button after calculation (refer to ③ in Fig. 7-35), its result is saved although the Area window is closed. (4) The result of calculation is reset by clicking the (reset) button on the Processing toolbar with the data processing window displayed. Upon calling up the Area window again, therefore, “*****” will be indicated in each result box. NOTE: Upon execution of file saving after area calculation, its result is also saved in a file. So a numerical value is indicated in each result box when the Area window is called up. These values cannot be erased by clicking the reset button because they are saved in the computer.
7.12 Area Calculation 7 - 32 For expanding the scale in the Area window, use the left button of the mouse. (1) As shown in Fig. 7-37, specify an area to be expanded by drawing a rectangle with the mouse while holding down its left button on a spectrum. Fig. 7-37 (2) Then release the left button. The specified area will be expanded as shown in Fig. 7-38. Fig. 7-38 (3) Double clicking the left mouse button on the spectrum returns the scale to the original one. 7.12.3 Scale Expansion
7.13 7 - 33 7.13 Transfer of Data to Microsoft(R) Excel Measured data can be transferred to a worksheet of Microsoft(R) Excel in the following procedure. (1) Click the (properties) button and select the Report tab. A tab page as in Fig. 7-39 will open. Select “Use Microsoft(R) Excel” for Output. Also select data to be transferred (peak table, data listing, etc. in case of wavelength scan). Then click OK. Fig. 7-39 (2) Click the (report) button. The Microsoft(R) Excel will automatically start to read data. The subsequent procedure concerns the Microsoft(R) Excel operation. Fig. 7-40
7.13 Transfer of Data to Microsoft(R) Excel 7 - 34 (3) Data can be saved with extension code “xls” of Microsoft(R) Excel. Select File – Save Copy As and enter a file name. Fig. 7-41 (4) Upon closing the Microsoft(R) Excel, a window as in Fig. 7-42 appears. Now close this window. The original data processing window will appear again. Fig. 7-42
7.14 7 - 35 7.14 Pasting of Spectrum to Microsoft(R) Word A spectrum can be pasted to a Microsoft(R) Word text as it is. Take the following procedure. (1) Make spectral data active by clicking the relevant spectrum in the data processing window. The thick frame will change to blue. (2) Select the Copy command from the Edit menu. (3) Open a Microsoft(R) Word text and click a position for pasting. By clicking the Paste command from the Edit menu, the spectrum can be pasted to the Microsoft(R) Word text. Peak tables and/or kinetics data can also be pasted to a Microsoft(R) Word text in the same way. In this case, they are pasted as a text. By double-clicking the pasted spectrum, functions such as scale change and gridline indication are applicable. Fig. 7-43 Spectrum Pasted to Microsoft(R) Word Upon right-clicking the pasted spectrum, a menu box as in Fig. 7-48 will appear to enable scale change, etc. Any change made here is brought into a Microsoft(R) Word text. NOTE: Editing in the Microsoft(R) Word can be made with a PC in which the Microsoft(R) Word and FL Solutions program are installed.
7.15 Pasting of Spectrum to Microsoft(R) Excel 7 - 36 7.15 Pasting of Spectrum to Microsoft(R) Excel A spectrum can be pasted to the Microsoft(R) Excel as it is. Take the following procedure. (1) Make spectral data active by clicking the relevant spectrum on the data processing window. The thick frame will change to blue. (2) Select the Copy command from the Edit menu. (3) Open the Microsoft(R) Excel and select “Paste Special” from its Edit menu. A dialog box as in Fig. 7-44 will open. Make sure that “FL Wavelength Scan Object” is indicated in “As.” Fig. 7-44 (4) Click OK. The spectrum will be pasted to the Microsoft(R) Excel. Peak tables and kinetics data can also be pasted to the Microsoft(R) Excel in the same way. In this case, they are pasted as data for each cell.