Hitachi F7000 Instruction Manual

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(7)  Set the diffuser into the cell holder in the sample compartment.   
(Refer to Fig. F-12.) 
 
 
 
Fig. F-12 
 
 
(8)  Execute the Instrumental Response command from the Utility 
menu.    The Instrumental Response dialog box will open as 
shown in Fig. F-13. 
Select “EX [500 to 900 nm].”    (Refer to ①  in Fig. F-13.) 
 
 
 
Fig. F-13 
 
 Diffuse
r 
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(9) Click the Run button.  (Refer to ② in Fig. F-13.)  A message 
box “Set diffuser to cell holder.” will appear.    Click the OK button.   
(Refer to ③  in Fig. F-14.) 
 
 
 
Fig. F-14 
 
 
(10)  The EX and EM wavelengths are set at 498.6 nm and the 
instrument starts synchronous wavelength scan (from 498.6 to 
901.4 nm). 
At this stage, the following measuring conditions are automatically 
set. 
• Scan mode :  Emission 
• Data mode :  Luminescence 
•  EX slit  :  5.0 nm 
•  EM slit  :  5.0 nm 
•  Scan speed  :  60 nm/min 
•  PMT voltage :  350 V 
• Response  :  Auto 
 
(11)  Upon completion of measurement, the conditions set before 
measurement are retrieved. 
Now, the instrument response on the emission side has been 
measured and stored.    Take out the diffuser. 
 
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F.5    Resetting of Instrumental Response 
 
(1) Click the Reset button in the Instrumental Response dialog box.   
(Refer to ①  in Fig. F-15.) 
 
 
 
Fig. F-15 
 
 
(2)  A message box “Erase instrumental response on 
excitation/emission side. OK?” will appear.    Click the OK button.   
(Refer to ②  in Fig. F-16.) 
 
 
 
Fig. F-16 
 
 
(3)  Now, the instrumental response has been erased. 
 
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APPENDIX G  PARAMETER SETTING RANGE 
FOR ANALYSIS METHOD 
 
G.1  General Tab:  Common to all measurement 
modes 
 
Parameter Name  Setting Range 
Measurement  Wavelength scan, Time scan, Photometry, 3-D scan,   
3-D Time scan 
Operator  Up to 20 characters 
Comments  Up to 255 characters 
Use sample table  ON, OFF 
 
ON :  Checked 
OFF :  Unchecked 
 
 
G.1.1  Wavelength Scan:  Instrument Tab 
 
Parameter Name  Setting Range 
Scan mode  Excitation, Emission, Synchronous 
Data mode  Fluorescence, Luminescence, Phosphorescence 
Chopping speed  10 Hz, 20 Hz, 40 Hz 
EX WL  
Scan Mode  Input Range 
Excitation  0, 200 to 900 nm 
Synchronous  200 to 890 nm   
EX Start WL  Input Range:    200 to 890 nm 
EX End WL  Input Range:    210 to 900 nm 
EM WL  Input Range:    0, 200 to 900 nm 
EM Start WL  Input Range:    200 to 890 nm 
EM End WL  Input Range:    210 to 900 nm  
						

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(cont’d) 
Parameter Name  Setting Range 
Scan speed   
(unit: nm/min) 60000 
30000 
12000 
2400 
1200 
240 
60 
30 
When Synchronous is specified for scan mode, 1200 nm/min or less 
is indicated. 
When Phosphorescence is specified for data mode, select from the 
following.   
Chopping speed is in parentheses. 
F-7000 (40 Hz)  F-7000 (20 Hz/10 Hz) 
240 
60 
30 60 
30 
  
Delay  0 to 9999 s 
EX Slit (unit: nm)  1.0 
2.5 
5.0 
10.0 
20.0 
EM Slit (unit: nm)  1.0 
2.5 
5.0 
10.0 
20.0 
PMT Voltage  Setting range:    250, 400, 700, 950 V 
0 to 1000 V when “PMT Voltage 0-1000V” is turned ON (checked). 
PMT Voltage   
0 - 1000 V ON, OFF 
Response (unit: s)  0.002 
0.004 
0.01 
0.05 
0.1 
0.5 
2.0 
4.0 
8.0 
Auto 
Corrected spectra  ON, OFF 
Shutter control  ON, OFF 
Replicates  1 to 99 
Cycle time  0.0 to 180.0 min 
 
ON :  Checked 
OFF :  Unchecked  
						

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G.1.2  Wavelength Scan:  Monitor Tab 
 
Parameter Name  Setting Range 
Y Axis: Max  -119999999.9 to 120000000.0 
Y Axis: Min  -120000000.0 to 119999999.9 
Open data processing window after data acquisition  ON, OFF 
Open data processing window after acquisition 
(Overlay) ON, OFF 
Print report after data acquisition  ON, OFF 
Overlay ON, OFF 
 
 
G.1.3  Wavelength Scan:  Processing Tab 
 
Parameter Name  Setting Range 
CAT ON, OFF 
Processing choices  Savitsky-Golay, Smooth, Mean Smooth,   
Median Smooth, Derivative 
Peak Finding: Integration method  Rectangular, Trapezoid, Romberg 
Peak Finding: Threshold  0.001 to 1000 
Peak Finding: Sensitivity  1, 2, 4, 8 
 
  
						

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G.1.4  Wavelength Scan:  Report Tab 
 
Parameter Name  Setting Range 
Output  Print Report, Use Microsoft(R) Excel,   
Use print generator sheet 
Orientation Portrait, Landscape 
Print items: Include date  ON, OFF 
Print items: Include method  ON, OFF 
Print items: Include graph  ON, OFF 
Print items: Include data listing  ON, OFF 
ON:    “Constant” or “Select data” is selectable. 
When “Constant” is specified: 
Parameter Setting Range 
Data interval  Minimum sampling interval to 
measuring wavelength interval 
Data start  Wavelength range on 
Instrument tab page 
Data end  Wavelength range on 
Instrument tab page 
When “Select data” is specified: 
Selectable from 12 wavelengths. 
Print items: Include peak table  ON, OFF 
ON:    Items to be output peak table are selectable. 
Item Setting Range 
Peak WL/Peak data  ON, OFF 
Start WL/End WL  ON, OFF 
Valley WL/Valley data  ON, OFF 
Peak Area  ON, OFF   
 
  
						

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G.2.1  Time Scan:  Instrument Tab 
 
Parameter Name  Setting Range 
Data mode  Fluorescence, Luminescence, Phosphorescence,   
Phosphorescence Life Time,   
Phosphorescence Life Time (short)   
Chopping speed  10 Hz, 20 Hz, 40 Hz 
EX WL  Input Range:    0, 200 to 900 nm 
EM WL  Input Range:    0, 200 to 900 nm 
Time unit  s, ms 
Scan time  
Time Unit  Input Range 
s  10 to 9000 s 
ms  100 to 9999 ms 
 
When Phosphorescence Life Time (short) is specified for data 
mode: 
Chopping Speed  Scan Time 
40 Hz  Fixed at 20 ms 
20 Hz  Fixed at 40 ms 
10 Hz  Fixed at 80 ms   
Delay  0 to 9999 s 
EX Slit (unit: nm)  1.0 
2.5 
5.0 
10.0 
20.0 
EM Slit (unit: nm)  1.0 
2.5 
5.0 
10.0 
20.0 
PMT Voltage  Setting range:    250, 400, 700, 950 V 
0 to 1000 V when “PMT Voltage 0-1000V” is turned ON (checked). 
PMT Voltage   
0 - 1000 V ON, OFF 
Response (unit: s)  0.002 
0.004 
0.01 
0.05 
0.1 
0.5 
2.0 
4.0 
8.0 
Shutter control  ON, OFF 
Replicates  1 to 99 
Cycle time  0.0 to 180.0 min 
  
						

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G.2.2  Time Scan:  Monitor Tab 
 
Parameter Name  Setting Range 
Y Axis: Max  -119999999.9 to 120000000.0 
Y Axis: Min  -120000000.0 to 119999999.9 
X Axis: Max (unit: s)  0.1 to 9999.0 
X Axis: Min (unit: s)  0.0 to 9998.9 
Open data processing window after 
data acquisition ON, OFF 
Open data processing window after 
acquisition (Overlay) ON, OFF 
Print report after data acquisition  ON, OFF 
Overlay ON, OFF 
 
 
G.2.3  Time Scan:  Processing Tab 
 
Parameter Name  Setting Range 
Processing choices  Savitsky-Golay Smooth, Mean Smooth,   
Median Smooth, Derivative 
Peak Finding: Integration method  Rectangular, Trapezoid, Romberg 
Peak Finding: Threshold  0.001 to 1000 
Peak Finding: Sensitivity  1, 2, 4, 8 
Kinetics: Start time  0 to 3599 s, 0 to 9998 ms 
Kinetics: End time  0 to 3600 s, 1 to 9999 ms 
Kinetics: K-Factor  -9999 to -0.001, 0.001 to 9999 
  
						

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G.2.4  Time Scan:  Report Tab 
 
Parameter Name  Setting Range 
Output  Print Report, Use Microsoft(R) Excel,   
Use print generator sheet 
Orientation Portrait, Landscape 
Print items: Include date  ON, OFF 
Print items: Include method  ON, OFF 
Print items: Include graph  ON, OFF 
Print items: Include data listing  ON, OFF 
ON: “Constant” or “Select data” is selectable. 
When “Constant” is specified: 
Parameter Setting Range 
Data interval  Minimum sampling interval to 
measuring scan time 
Data start  Scan time range on 
Instrument tab page 
Data end  Scan time range on 
Instrument tab page 
When “Select data” is specified: 
Selectable from 12 time points (in measurement 
range). 
Print items: Include peak table  ON, OFF 
ON:    Items to be output onto peak table are selectable.
Item Setting Range 
Peak time/Peak data  ON, OFF 
Start time/End time  ON, OFF 
Valley time/Valley data  ON, OFF 
Peak Area  ON, OFF   
Print items: Include kinetics  ON, OFF