Hitachi F7000 Instruction Manual
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APPENDIX H SAMPLING INTERVAL
H.1 Wavelength Scan
The data sampling interval is set as follows according to the scan speed.
[Data mode = Fluorescence/Luminescence]
Scan Speed (nm/min) Sampling Interval (nm)
60000 2.0
30000 1.0
12000 0.2
2400 0.2
1200 0.2
240 0.2
60 0.2
30 0.2
[Data mode = Phosphorescence, Chopping speed = 40 Hz]
Scan Speed (nm/min) Sampling Interval (nm)
240 0.2
60 0.2
30 0.2
[Data mode = Phosphorescence, Chopping speed = 20/10 Hz]
Scan...](http://img.usermanuals.tech/thumb/33/65127/w64_hitachi-f7000-instruction-manual-1492072981_d-372.png "Page 373
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APPENDIX H SAMPLING INTERVAL
H.1 Wavelength Scan
The data sampling interval is set as follows according to the scan speed.
[Data mode = Fluorescence/Luminescence]
Scan Speed (nm/min) Sampling Interval (nm)
60000 2.0
30000 1.0
12000 0.2
2400 0.2
1200 0.2
240 0.2
60 0.2
30 0.2
[Data mode = Phosphorescence, Chopping speed = 40 Hz]
Scan Speed (nm/min) Sampling Interval (nm)
240 0.2
60 0.2
30 0.2
[Data mode = Phosphorescence, Chopping speed = 20/10 Hz]
Scan...")
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H.2 Time Scan
The data sampling interval is set as follows according to the scan time.
[Data mode = Fluorescence/Luminescence/Phosphorescence/
Phosphorescence Life Time]
Sampling Interval [ms]
Phosphorescence
Mode/Chopping Speed Scan Time
[s] Fluorescence/Luminescence/
Phosphorescence
Life Time Mode
40 Hz 20 Hz 10 Hz
0.1 ~ 1 1 50 100 200
~ 3 1 50 100 200
~ 7 2 50 100 200
~ 18 5 50 100 200
~ 36 10 50 100 200
~ 72 20 50 100 200
~ 180 50 50 100...](http://img.usermanuals.tech/thumb/33/65127/w64_hitachi-f7000-instruction-manual-1492072981_d-373.png "Page 374
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H.2 Time Scan
The data sampling interval is set as follows according to the scan time.
[Data mode = Fluorescence/Luminescence/Phosphorescence/
Phosphorescence Life Time]
Sampling Interval [ms]
Phosphorescence
Mode/Chopping Speed Scan Time
[s] Fluorescence/Luminescence/
Phosphorescence
Life Time Mode
40 Hz 20 Hz 10 Hz
0.1 ~ 1 1 50 100 200
~ 3 1 50 100 200
~ 7 2 50 100 200
~ 18 5 50 100 200
~ 36 10 50 100 200
~ 72 20 50 100 200
~ 180 50 50 100...")
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H.3 3-D Time Scan
The data sampling interval is set as follows according to the scan speed.
[Data mode = Fluorescence/Luminescence]
Scan Speed (nm/min) Sampling Interval (nm)
60000 2.0
30000 1.0
12000 1.0
2400 1.0
1200 1.0
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 40 Hz]
Scan Speed (nm/min) Sampling Interval (nm)
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 20/10 Hz]
Scan Speed (nm/min) Sampling Interval (nm)...](http://img.usermanuals.tech/thumb/33/65127/w64_hitachi-f7000-instruction-manual-1492072981_d-374.png "Page 375
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H.3 3-D Time Scan
The data sampling interval is set as follows according to the scan speed.
[Data mode = Fluorescence/Luminescence]
Scan Speed (nm/min) Sampling Interval (nm)
60000 2.0
30000 1.0
12000 1.0
2400 1.0
1200 1.0
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 40 Hz]
Scan Speed (nm/min) Sampling Interval (nm)
240 1.0
60 1.0
30 1.0
[Data mode = Phosphorescence, Chopping speed = 20/10 Hz]
Scan Speed (nm/min) Sampling Interval (nm)...")
A - 58 (cont’d) Parameter Name Setting Range Response (unit: s) 0.002 0.004 0.01 0.05 0.1 0.5 2.0 4.0 8.0 Auto Corrected spectra ON, OFF Shutter control ON, OFF Replicates 2 to 99 Cycle time 2 to 3600 s ON : Checked OFF : Unchecked G.5.2 3-D Time Scan: Monitor Tab Parameter Name Setting Range Y Axis: Max -119999999.9 to 120000000.0 Y Axis: Min -120000000.0 to 119999999.9 Contour interval 0.001 to 1000 Open data processing window after data acquisition ON, OFF Print report after data acquisition ON, OFF G.5.3 3-D Time Scan: Processing Tab Parameter Name Setting Range Peak Finding: Integration method Rectangular Peak Finding: Threshold 0.001 to 1000 Peak Finding: Sensitivity 1, 2, 4, 8
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G.5.4 3D Time Scan: Report Tab
Parameter Name Setting Range
Output Print Report, Use Microsoft(R) Excel, Use print
generator sheet
Orientation Portrait, Landscape
Print items: Include date ON, OFF
Print items: Include instrument ON, OFF
Print items: Include graph ON, OFF
ON: Any of the following items are selectable.
• Contour
• Bird’s-eye view
• Contour and Bird’s-eye view
• Contour and Spectrum
Print items: Data listing*1 ON, OFF
ON: Setting can be made for time scan and
wavelength scan spectra.
Parameter Setting Range
Interval Minimum data interval to measuring
wavelength interval
Start Wavelength range on Instrument tab
page
End Wavelength range on Instrument tab
page.
*1: Valid when “Use Microsoft(R) Excel” or “Use print generator sheet” is specified for
Output. Always turned ON when “Use print generator sheet” is specified.
A - 60 APPENDIX H SAMPLING INTERVAL H.1 Wavelength Scan The data sampling interval is set as follows according to the scan speed. [Data mode = Fluorescence/Luminescence] Scan Speed (nm/min) Sampling Interval (nm) 60000 2.0 30000 1.0 12000 0.2 2400 0.2 1200 0.2 240 0.2 60 0.2 30 0.2 [Data mode = Phosphorescence, Chopping speed = 40 Hz] Scan Speed (nm/min) Sampling Interval (nm) 240 0.2 60 0.2 30 0.2 [Data mode = Phosphorescence, Chopping speed = 20/10 Hz] Scan Speed (nm/min) Sampling Interval (nm) 60 0.2 30 0.2
A - 61 H.2 Time Scan The data sampling interval is set as follows according to the scan time. [Data mode = Fluorescence/Luminescence/Phosphorescence/ Phosphorescence Life Time] Sampling Interval [ms] Phosphorescence Mode/Chopping Speed Scan Time [s] Fluorescence/Luminescence/ Phosphorescence Life Time Mode 40 Hz 20 Hz 10 Hz 0.1 ~ 1 1 50 100 200 ~ 3 1 50 100 200 ~ 7 2 50 100 200 ~ 18 5 50 100 200 ~ 36 10 50 100 200 ~ 72 20 50 100 200 ~ 180 50 50 100 200 ~ 360 100 100 100 200 ~ 720 200 200 200 200 ~ 1800 500 500 500 1000 ~ 3600 1000 1000 1000 1000 ~ 7200 2000 2000 2000 2000 ~ 9000 5000 5000 5000 5000 [Data mode = Phosphorescence Life Time (short)] Chopping Speed [Hz] Scan Time [ms] Sampling Interval [µs] 40 20 10 20 40 20 10 80 40
A - 62 H.3 3-D Time Scan The data sampling interval is set as follows according to the scan speed. [Data mode = Fluorescence/Luminescence] Scan Speed (nm/min) Sampling Interval (nm) 60000 2.0 30000 1.0 12000 1.0 2400 1.0 1200 1.0 240 1.0 60 1.0 30 1.0 [Data mode = Phosphorescence, Chopping speed = 40 Hz] Scan Speed (nm/min) Sampling Interval (nm) 240 1.0 60 1.0 30 1.0 [Data mode = Phosphorescence, Chopping speed = 20/10 Hz] Scan Speed (nm/min) Sampling Interval (nm) 60 1.0 30 1.0
A - 63 APPENDIX I PARAMETER SETTING RANGE ON OPTIONS WINDOW I.1 Display Tab Parameter Name Setting Range Show trace display ON, OFF Show gridlines with increment ON, OFF ON: Gridline width is selectable from Wide, Middle and Narrow. Display items: Wavelength scan Show graph only, Show graph plus peak table Display items: Time scan Show graph only, Show graph plus kinetic data, Show graph plus peak table Display items: 3-D Scan Show contour plus EX, EM spectrum, Show contour plus EX, EM spectrum plus peak table Display items: Intracellular cation scan Show graph only, Show graph plus kinetic data, Show graph plus mean, Show graph plus point Display items: Intracellular cation spectrum Show fluorescence only, Show fluorescence plus ratio, Show fluorescence plus concentration, Show fluorescence plus ratio plus concentration Display items: Polarization time scan Show Graph only, Show graph plus kinetic data, Show graph plus point Display items: Polarization spectrum Show fluorescence only, Show fluorescence plus polarization degree, Show fluorescence plus anisotropy, Show fluorescence plus polarization degree plus anisotropy
A - 64 I.2 Processing Tab Parameter Name Setting Range Integration method Rectangular, Trapezoid, Romberg Calibration formula Data = f(Conc), Conc = f(Data) Data output to peak table ON, OFF Smoothing: Processing Savitsky-Golay, Mean, Median Smoothing: Order 2 to 4 Smoothing: Number of points 5 to 101 Derivative: Smoothing order 2 to 4 Derivative: Smoothing order 5 to 101 I.3 Analysis Condition Tab Parameter Name Setting Range Integration time for current data 0.1 to 10.0 s Change Instrumental Response Condition ON, OFF
A - 65 APPENDIX J ERROR MESSAGE LIST Error Message Probable Cause Remedy ROM Error ROM error has occurred. Contact our local service office. RAM Error RAM error has occurred. Contact our local service office. Lamp is not lighted Lamp extinguished during initialization. Replace the lamp. Power interruption Momentary power failure detected at instrument. Check the connection of USB cable, and start up PC and spectrophotometer again. If error recurs, contact our local service office. Zero adjust error! Faulty chopper operation Contact our local service office. Auto Pre-scan error! Peak not Found! Peak was not detected in auto pre-scan. Peak may be detected by widening the slit or increasing photomultiplier voltage. Pre-scan error! Peak not Found! Peak was not detected in normal pre-scan. Peak may be detected by widening the slit or increasing photomultiplier voltage. Wavelength Initialization error (EX)! Initialize position of EX side wavelength feed screw cannot be detected. Contact our local service office. Wavelength Initialization error (EM)! Initialize position of EM side wavelength feed screw cannot be detected. Contact our local service office. Shutter Initialization error Initialize position of shutter cannot be detected. Contact our local service office. EX Slit initialization error! Initialize position of EX side slit cannot be detected. Contact our local service office. EM Slit initialization error! Initialize position of EM side slit cannot be detected. Contact our local service office. Chopper error! Sync signal from chopper cannot be detected. Contact our local service office. Progam status error Abnormality detected in spectrophotometer main unit program. Contact our local service office. The Xe lamp was turned off suddenly. Turn off the main switch of the spectrophotometer unit. Xe lamp has burned out. Replace with a new Xe lamp.
INDEX - 1 INDEX 1st order (linear calibration curve) ............................................................................................... 4-12, A-1 3-D scan ........................................................................................................................................ 1-2, 5-1 3-D time scan ....................................................................................................................... 1-2, 1-27, 6-1 3rd order (Cubic working curve) .................................................................................................. 4-13, A-2 A ASCII text file ...................................................................................................................... 1-3, 7-37, 8-21 ASCII type 1 file .................................................................................................................................. 7-40 ASCII type 2 file .................................................................................................................................. 7-41 Activity ......................................................................................................................................... 3-22, A-7 Analog output ............................................................................................................................ 1-45, 9-12 Analysis Condition tab ............................................................................................................... 1-40, A-64 Area calculation/Area integration method ........................................................................ 1-26, 1-35, 7-27 Auto statistic calculation ..................................................................................................................... 4-17 Automatic pre-scanning (Auto Pre-scan) ........................................................................................... 1-43 Autoscale Y Axis................................................................................................................................. 8-13 B Bird’s eye view.................................................................................................................... 1-2, 5-12, 6-12 Blank measurement..............................................................................................................4-30, 9-1, 9-5 C CAT (Computing for Averaging Transient) ......................................................................................... 2-11 CD-ROM drive ...................................................................................................................................... 1-5 CPU ...................................................................................................................................................... 1-5 CV (coefficient of variation) .............................................................................................. 4-17, 4-38, 4-42 Calibration curve................................................................................................................................. 4-46 Calibration type................................................................................................................................... 4-11 Change of scale.................................................................................................................................... 1-3 Check box........................................................................................................................................... 1-19 Chopping speed .....................................................................................................2-6, 3-5, 4-16, 5-5, 6-5 Colors tab ........................................................................................................................................... 1-36 Combo box ......................................................................................................................................... 1-18 Command ........................................................................................................................................... 1-16 Concentration unit .............................................................................................................................. 4-14
INDEX - 2 Contour ...................................................................................................................... 1-2, 1-25, 5-19, 6-18 Contour interval .................................................................................................................................... 5-8 Corrected spectrum ............................................................................................. 1-28, 2-7, 5-7, 6-7, A-26 Cycle time ...................................................................................................................................... 2-8, 3-7 D DDE function ........................................................................................................................................ 1-2 Data menu ......................................................................................................2-26, 3-26, 4-44, 5-25, 6-22 Delay .................................................................................................................................... 2-6, 3-6, 4-17 Derivative................................................................................................................................... 7-17, 7-50 Determination coefficient ..............................................................................................4-38, A-7, A-8, A-9 Dialog box.................................................................................................................................. 1-18, 4-46 Differentiation/Derivative ................................................................................................... 1-3, 1-23, 1-35, Digit after decimal point ...................................................................................................................... 4-15 Display tab ................................................................................................................................. 1-32, A-63 E EX Power Monitor............................................................................................................................... 1-44 Emission spectrum ............................................................................................................................. 2-21 Error message ........................................................................................................................... 1-29, A-65 Event log............................................................................................................................................. 8-10 Excitation spectrum ................................................................................................................... 2-21, A-19 Exit ...................................................................................................................................................... 1-47 F FD drive ................................................................................................................................................ 1-5 File menu ................................................................................................................. 2-22, 3-20, 4-31, 5-15 Fonts tab............................................................................................................................................. 1-34 Force curve through zero ................................................................................................................... 4-15 Formula of calibration curve/Include calibration data ......................................................................... 4-12 Function key .............................................................................................................................. 1-24, 1-27 Fundamental arithmetic calculations .................................................................................................. 1-26 G General tab ...............................................................................................................2-3, 3-3, 4-3, 5-3, 6-3 H Hard disk .............................................................................................................................................. 1-5