Hitachi F7000 Instruction Manual

							6.1 
6 - 1 
6.  3-D TIME SCAN  
 
6.1  Flow of Operation  
 
This section explains the parameter setting and operating method for 3-
D time scan.    Measurement proceeds along the following flow.   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Fig. 6-1    Operational Flow (3-D time scan) Click 
   button and set your analytical conditions. 
 
 • Scan range 
• Data mode 
•  Scan speed, etc. 
Click 
 button. 
 • Sample name 
• Auto File 
•  File name, etc. 
Set a sample. 
Click 
  button to execute wavelength scan. 
• Spectrum tracing 
• Peak detection 
Click 
  button.    Measurement result will be displayed 
in a report format. 
 
Start 
Method setting 
(See 6.2.) 
Sample name/ 
comment input 
(See 6.3.) 
Measurement 
(See 6.4.) 
Data processing 
(See Section 7.) 
Result output in report 
(See 6.9.) 
End  
						

							6.2    Creating an Analysis Method 
6 - 2 
6.2    Creating an Analysis Method   
 
Set the analytical conditions (method) prior to measurement.     
 
(1) Click the 
   button on the Measurement toolbar.    The window 
in Fig. 6-2 appears.   
(Or select the Method command from the Edit menu.)     
 
 
 
Fig. 6-2  Analysis Method  
 
 
(2)  On the Analysis Method window, analytical conditions for the 3-D 
time scan mode can be set.    The following tabs are provided on 
this window.    These tabs are detailed on the subsequent pages.   
• General  
• Instrument  
• Monitor  
• Processing  
• Report  
 
  
						

							6.2.1 
6 - 3 
The parameters shown in Fig. 6-3 are provided at the General tab.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 6-3  General Tab 
 
 
1 Measurement (mode)   
A measurement mode is selectable.    Here, select “3-D Time 
scan.”  
For Wavelength scan, Time scan, Photometry and 3-D Scan, refer 
to Sections 2, 3, 4 and 5, respectively.   
2 Operator  
Enter an operator name.   
3 Instrument  
The name of the connected instrument is indicated.   
4 Sampling 
- Standard   
Indicates that samples are to be exchanged manually.   
5 Comments  
Enter a description or notes on measuring conditions.   
6.2.1 General Tab 
 
1 
2 
3 
4 
5 
6 
7 
8  
						

							6.2    Creating an Analysis Method 
6 - 4 
6 Load  
Click this button to read out saved analytical conditions.     
Upon clicking, the file opening window appears.    So, select an 
analytical condition file.   
7 Save  
When you click this button after setting the parameters, the 
analytical conditions can be saved by overwriting.    In case of a 
new analysis method, clicking this button displays the “Save As” 
window.    You must enter a file name for saving in this window.   
8 Save As   
Click this button for renaming the set conditions for saving.     
The “Save As” window appears, so enter a file name.   
 
  
						

							6.2.2 
6 - 5 
The window shown below is based on that 3-D Time scan is specified 
for Measurement at the General tab.    When you click the Instrument 
tab, the window in Fig. 6-4 appears.   
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 6-4  Instrument Tab 
 
 
1 Scan mode   
Select a scan mode here.    In case of Synchronous, the abscissa 
of the graph stands for excitation wavelength.    The unit indication 
of the abscissa becomes ‘EX/EM (nm).’   
2 Data mode   
Select a data mode.    The Phosphorescence mode is for acquiring 
only phosphorescence after extinction of excitation beam while 
turning a chopper on the excitation side.   
3 Chopping speed   
Select a chopping speed.    Setting is allowed when 
Phosphorescence is selected for Data mode.     
The Phosphorescence mode is for acquiring only phosphorescence 
after extinction of excitation beam while turning a chopper on the 
excitation side.    An increase in chopping speed makes the 
chopper turns faster.   
6.2.2 Instrument 
Tab 
3 
4 
5 
6 
7 
2 
1 
8 
9 
21 10 
13
14
15
16
17
12
11
18
19
20 
						

							6.2    Creating an Analysis Method 
6 - 6 
4 EM WL   
For the Excitation scan mode, enter the fixed wavelength on the 
emission side .    For the Synchronous scan mode, enter the start 
wavelength on the emission side.   
5 EX Start WL   
This is the start wavelength on the excitation side in wavelength 
scan.    Enter the shorter wavelength side of measuring wavelength 
range.  
6 EX End WL   
This is the end wavelength on the excitation side in wavelength 
scan.    Enter the longer wavelength side of measuring wavelength 
range.  
7 EX WL   
For the Emission scan mode, enter the fixed wavelength on the 
excitation side.   
8 EM Start WL   
This is the start wavelength on the emission side in wavelength 
scan.    Enter the shorter wavelength side of measuring wavelength 
range.  
9 EM End WL   
This is the end wavelength on the emission side in wavelength 
scan.    Enter the longer wavelength side of measuring wavelength 
range.  
10 Scan speed   
Set a scan speed.   
11 EX Slit   
Select a slit width on the excitation side.   
12 EM Slit   
Select a slit width on the emission side.   
13 PMT Voltage   
A function for controlling the voltage of the photomultiplier detector 
(photomultiplier tube).    As a reference, changing from 400 V to 
700 V increases the data value about two digits and changing from 
700 V to 950 V increases it about 1 digit.   
When a check mark is put for PMT Voltage 0-1000V, the 
photomultiplier voltage is settable within a range from 0 to 1000 V.   
14 PMT Voltage 0-1000V   
When a check mark is put in this box, the photomultiplier voltage is 
settable within a range from 0 to 1000 V.    
						

							6.2.2 
6 - 7 
15 Response  
When “Auto” is selected, response will be set automatically 
depending on scan speed.    Select “Auto” usually.   
16 Corrected spectra   
A function for determining the spectrum inherent to a sample by 
correcting the photometer wavelength characteristic using the 
saved instrument parameters, following measurement with the 
instrument parameters for photometer control.    If there are no 
instrument parameters for the wavelength range to be measured, 
only the wavelength range that allows correction is scanned.     
And if there is no wavelength area measurable, an error will occur.   
The Instrument Response command is provided in the Utility menu 
for operating this function.   
When this setting is at ON, “Corrected spectra” appears on the 
monitor window.    (See Fig. 1-43 in 1.6.)   
17 Shutter control   
The shutter can automatically be closed in other than measurement 
for slowing down sample deterioration due to the energy of 
excitation beam and opened when measurement starts.     
18 Replicates  
Set the number of repeat measurements.     
19 Cycle time   
Set a repetition interval.   
20 Range of corrected spectra   
Spectrum correction range is indicated.   
21 Prediction measurement time   
The predicted measurement time is indicated.    After change of a 
parameter such as scan speed, click the Update button.   
The predicted value will then be updated.   
 
  
						

							6.2    Creating an Analysis Method 
6 - 8 
When selecting the Monitor tab, the window in Fig. 6-5 appears.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 6-5  Monitor Tab  
 
 
Assign the following parameters.   
 
1 Y-Axis Max (maximum)   
Enter a maximum value for Y-axis on the monitor window.   
2 Y-Axis Min (minimum)   
Enter a minimum value for Y-axis on the monitor window.   
3 Contour interval   
Function for setting intervals of drawing contour lines.     
4 Open data processing window after data acquisition   
Select whether or not to conduct data processing after sample 
measurement.    When selected (check mark is applied), an icon 
(as shown in 
) is displayed for the data 
processing window at the end of measurement.    And by opening 
this icon, data processing such as peak detection is available.   
 
6.2.3 Monitor Tab 
 
2 
1 
4 
5 
3  
						

							6.2.3 
6 - 9 
NOTE:  When the remaining memory capacity of PC in operation is 
very small (system resource is less than 20%), the data 
processing window will not open even if this item is turned ON.   
Either close the presently opened data processing window or 
restart the FL Solutions program.   
 
5 Print report after data acquisition   
Select whether or not to print a report after sample measurement.   
When selected (check mark is applied), printing will be made 
automatically after measurement.    The items set at the Report tab 
will be printed.    When this item is not selected, data processing is 
allowed by reading out a file via [File] - [Open].   
 
  
						

							6.2    Creating an Analysis Method 
6 - 10 
On selection of the Processing tab, the window in Fig. 6-6 will be 
displayed.  
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 6-6  Processing Tab  
 
 
1 Peak Finding:  Integration method  
Select an integration method for the peaks to be displayed in the 
data processing window.   
2 Threshold  
Set a detection limit for the photometric value axis of peak and 
valley.    Peaks or valleys below the setting will not be detected.   
3 Sensitivity  
Select the number of data points in the horizontal axis direction 
(abscissa).    For detecting sharp peaks, select a sensitivity value of 
“1.”    Or select “8” for detecting broad peaks.   
 
 
6.2.4 Processing 
Tab 
 
1 
3
2