Hitachi F 2500 Manual

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2.5    Conducting a Wavelength Scan   
 
(1)  There are two methods of starting the scan.     
 
(a)  Select the Measure command from the 
Spectrophotometer menu.   
 
(b)  Click the     (measurement) button on the 
measurement toolbar.   
For halting the measurement midway, click the   
button on the toolbar.   
 
(2)  Upon clicking the          button, the window shown in   
Fig. 2-10 appears when using a sample table.    Now click 
Yes.  
 
 
 
Fig. 2-10 
 
 
(3)  The dialog box as in Fig. 2-11 will open.    Set each sample 
and click OK.  
 
 
 
Fig. 2-11 
 
2.5    Conducting a Wavelength Scan  
						

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(4)  When automatic saving is set, data will be saved after 
completion of measurement.    Upon opening its file, a data 
processing window will appear as in Fig. 2-12.     
Unless automatic saving is set, select the Save As 
command from the File menu and save data in file.   
 
 
 
Fig. 2-12    Data Processing Window (wavelength scan) 
 
 
(a) Spectrum  
The spectrum of a measured sample is traced.   
 
(b) Peak table  
Peak wavelength, peak height and valley wavelength 
are displayed.   
(a) Spectrum 
(b) Peak table 2.5  
						

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2.6  Data Printout  
 
Printout can be provided even when the function for automatic 
printout after measurement is not used.    The following two 
procedures are available.   
 
(1)  Select the Print command from the File menu.    Or click the   
          print button on the toolbar.    The field active on the 
data processing window (e.g., (a) in Fig. 2-12) will be 
printed.    What is displayed by clicking the Print Preview 
command in the File menu will be printed.     
 
 
 
Fig. 2-13 
 
 
2.6  Data Printout  
						

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(2)  Select the Report command in the Data menu.    Or click 
the     button on the toolbar.  The Print Preview window 
will appear, so click Print.    Select the items to be printed 
at the Report tab under Properties.   
 
 
 
Fig. 2-14 
 
 
NOTE:  If characters overflow from the print frame in 
repeat measurement or in overlaying, then change 
the printing orientation from vertical to horizontal.   
 
2.6  
						

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3.  TIME SCAN 
 
 
3.1  Flow of Operation  
 
This section explains the parameter setting and operating 
method for time scan.     
Measurement is performed in a flow shown below. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Fig. 3-1    Operational Flow (time scan) 
End 
Start 
Method setting 
Click  button.  
 Measuring 
wavelength 
 Scan time 
 
Sample name/comment input
Click  button.  
Measurement 
Data processing 
 Rate calculation 
 Peak detection 
 Spectrum tracing 
 Spectral calculation 
Print  (See page 3-2.) 
(See page 3-13.) 
 
(See page 3-15.)   
 Click 
 button.  
(Refer to Section 6.) 
Click 
 button.  
(See page 3-17.)     
 
 
3.1  
						

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3.2    Creating an Analysis Method   
 
Set the analytical conditions (method) prior to measurement.   
From the Edit menu, select the Method command or click the       
button.    The dialog box shown in Fig. 3-2 will appear.   
 
 
 
Fig. 3-2  Analysis Method 
 
 
The following tabs are provided on the Analysis Method window 
for the time scan mode.    These tabs are explained on the 
subsequent pages.   
• General 
• Instrument 
• Monitor 
• Processing 
• Report  
 
3.2    Creating an Analysis Method  
						

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(1) General  
 
The parameters shown in Fig. 3-3 are provided under the 
General tab.   
 
 
 
Fig. 3-3  General Tab 
 
 
(a) Measurement (mode)  
Select a measurement mode from among the four 
listed below.   
• Wavelength scan  
• Time scan  
• Photometry  
•  3-D Scan (displayed when the ‘3-D Scan program’ 
(option) is installed in combination with Model   
F-2500)  
 
Here, select “Time scan.”    For Wavelength scan, 
Photometry and 3-D Scan, refer to Sections 2, 4 and 5.   
 
(b) Operator  
Enter an operator name.     
 
(c) Instrument  
The model of the connected instrument is indicated.   
 
(d)  Use sample table (Set measurement sample) 
Apply a check mark here when a sample table is used.     
 
3.2  
						

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(e) Sampling  
• Standard  
When sample is exchanged manually.   
 
(f) Comments  
Enter a description or notes on measuring conditions.   
 
(g) Load  
Click this button to read out saved analytical conditions.   
The file opening window appears upon clicking, so 
select an analytical conditions file.   
 
(h) Save  
By clicking this button after setting the parameters for 
all five tabs, the analytical conditions are saved.   
Clicking this button overwrites parameters on the 
existing ones.   
 
(i) Save As  
Click this button for renaming the set conditions for 
saving.    The “Save As” window will appear, so enter a 
file name.   
 
(2) Instrument  
 
This window appears when Time scan is specified for 
Measurement under the General tab on the Analysis 
Method window.    When you click the Instrument tab, the 
window in Fig. 3-4 appears.   
 
 
 
Fig. 3-4  Instrument Tab 
3.2    Creating an Analysis Method  
						

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(a) Data mode  
Select one of the following data modes.   
The Luminescence mode is for measuring 
luminescence in the biological/chemical field and only 
the sample side signal is acquired without performing 
ratio photometry.   
If phosphorescence life is shorter than 20 ms, 
“Phosphorescence short life” should be selected.     
In this case, unit time and measuring time are 
automatically set to ms and 20 ms.     
• Fluorescence  
• Luminescence  
•  Phosphorescence (only with Model F-4500)   
•  Phosphorescence Life Time (only with Model   
F-4500)  
•  Phosphorescence Life Time (Short) (only with 
Model F-4500)   
 
(b) EX WL 
Input an excitation wavelength.   
 
Model Input Range  
F-4500  0, 200 to 900 nm 
F-2500  0, 200 to 800 nm 
 
 
(c) EM WL  
Input an emission wavelength.   
 
Model Input Range  
F-4500  0, 200 to 900 nm 
F-2500  0, 200 to 800 nm 
 
 
(d)  Time unit (only with Model F-4500) 
Set a time unit.   
• s  
• ms  
 
3.2  
						

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(e) Scan time  
Set a scan time.     
 
Model Input Range  
F-4500  10 to 3600 s   
1000 to 9999 ms   
F-2500  10 to 9000 s 
 
According to the set scan time, data sampling interval 
is set as listed below.   
 
• Model F-4500   
 
Measuring Time   
(s) Sampling Interval   
(ms) 
~ 3  1 
~ 7  2 
~ 18  5 
~ 36  10 
~ 72  20 
~ 180  50 
~ 360  100 
~ 720  200 
~ 1800  500 
~ 3600  1000 
 
In the Phosphorescence mode, the minimum sampling 
interval is 50 ms.    It is fixed at 40 µs in 
phosphorescence short-life measurement.   
 
• Model F-2500 
 
Measuring Time   
(s) Sampling Interval   
(ms) 
~ 120  100 
~ 240  200 
~ 600  500 
~ 1200  1000 
~ 2400  2000 
~ 6000  5000 
~ 9000  10000 
 
 
3.2    Creating an Analysis Method