Hitachi F 2500 Manual

							 
SAFETY - 2 
     SAFETY SUMMARY    
 
 
 
 
General Safety Guidelines (Continued) 
 
NOTICE :  Used to indicate an instruction for preventing 
damage to the product. 
NOTE  :  Used to indicate an instruction for ensuring 
correct use of the product and accurate analysis 
therewith. 
 
 
 Do not modify the instrument, replace parts that are not user-
serviceable, use non-specified parts, nor remove safety 
devices, as it could be hazardous.   
 Installation at delivery, maintenance and relocation should be 
referred to service personnel qualified by us.   
 Do not perform any operation or action other than described 
in this manual.    When in doubt, please contact our local 
sales representative or service office nearest you.   
 When using a chemical for analytical operation, be sure to 
provide proper ventilation in the laboratory room as per local 
requirements.    Inadequate ventilation could endanger your 
health.  
 Keep in mind that the hazard warnings in this manual or on 
the product cannot cover every possible case, as it is 
impossible to predict and evaluate all circumstances 
beforehand.  
Be alert and use your common sense.   
 
  
						

							 
SAFETY - 3 
     SAFETY SUMMARY    
 
 
 
 
Safety Notices on Fluorescence Spectrophotometer   
 
Electricity  
 
 Make sure that the fluorescence spectrophotometer is 
supplied with power of 100V AC, 1 kVA or more (50 Hz or   
60 Hz).    If the power voltage fluctuates or noise is applied to 
the power line, the spectrophotometer may be adversely 
affected, resulting in any trouble in it.   
 Be sure to provide grounding connection together with power 
line connection.    The grounding resistance should be 100 
ohms or less (conforming to Class-D grounding installation of 
electric facility technical standards).    Check that the 
grounding connection is proper.    If the grounding connection 
is improper, the spectrophotometer may become vulnerable to 
external noise or stray voltage may be produced in it, resulting 
in an electric shock hazard.   
 The high voltage circuit is used inside the spectrophotometer.   
To prevent electric shock, do not open covers during 
operation unless otherwise necessary.   
 
 
Source of Heat/Flame   
 
Do not smoke or hold a flame near the fluorescence 
spectrophotometer.  
 
 
Data Backup   
 
Data stored on the hard disk may become unusable due to a 
system failure, wrong operation, computer virus infection, etc.   
To ensure data integrity in case of accidental damage to the hard 
disk, periodically make backup copies of hard disk files onto 
floppy disks.   
To prevent an erroneous operation on the hard disk, always 
reserve approx. 100 MB of free space as a working area for 
application software.    
						

							 
SAFETY - 4 
     SAFETY SUMMARY   
 
 
 
 
Protection against Computer Viruses   
 
If any program/data is damaged suddenly or an unexpected 
operation/screen is encountered, the personal computer is 
suspected of being infected by a computer virus.     
Computer viruses are malicious programs that sneak into 
personal computers to cause misbehavior or damage to data.   
And, a program designed to offer protection against computer 
viruses is called a vaccine program.   
 
Possible causes of virus infection are:   
 Downloading a virus-laden program through communication.   
 Using a floppy disk or other storage medium infected by a 
virus.  
 
Note also that once any personal computer is infected by a virus, 
it may spread to other computers via communication or storage 
medium.  
Never use a program or storage medium that is suspected 
of containing a virus.   
If there is a possibility of virus infection, check for a virus using a 
vaccine program.    Note, however, that some kinds of vaccine 
programs cannot eradicate particular viruses.   So, be sure to 
make a backup of hard disk files beforehand.   
The user is requested to prepare a vaccine program and carry 
out virus removal on his or her own responsibility.   
 
 
Power Interruption   
 
On occurrence of momentary power voltage drop due to power 
interruption or lightning, the personal computer may become 
faulty or the system software, application software or data may 
be damaged.    For protection against momentary power voltage 
drop, it is advisable to use an AC uninterruptible power supply 
unit (stated according to the Japanese Electronic Industry 
Development Association guidelines for protection against 
momentary power voltage drop in personal computers).   
  
						

							 
SAFETY - 5 
     SAFETY SUMMARY   
 
 
 
 
Turning On/Off Personal Computer   
 
Do not turn off power to the personal computer while the hard 
disk or floppy disk drive is active.    If power to the personal 
computer is turned off while the hard disk or floppy disk is being 
accessed, the personal computer may become faulty or 
data/software stored in it may be damaged.   
Before turning off power to the personal computer, be sure to 
quit the fluorescence spectrophotometer control/data processing 
program (FL Solutions program) first and then take the shut-
down procedure using the system software.   
  
						

							 FOREWORD - 
1 
FOREWORD  
 
 This instruction manual is intended for the users of the Hitachi 
fluorescence spectrophotometer.    It describes the installation, 
maintenance, operation and measurement procedures.   
 The fluorescence spectrophotometer is designed for the 
purpose of fluorescence analysis.    Never use this instrument 
for any other purposes.   
 The fluorescence spectrophotometer is intended for use by 
persons having a basic knowledge of chemical analysis.   
Other persons should use them in the presence of a person 
familiar with chemical analysis.    Remember that improper 
use of analytical instruments, chemicals or samples would 
result not only in wrong analytical data but also in 
consequences adverse to safety.   
 Before attempting installation/maintenance of the 
fluorescence spectrophotometer, read the instruction manual 
carefully and fully understand the cautionary instructions for 
the sake of safety.   
 Keep this manual handy for easy reference when necessary.   
 The information contained in this manual is subject to change 
without prior notice.   
  
						

							 
- i -
CONTENTS 
 
 
PREFACE ......................................................................................................................... 1 
 
ABOUT THIS MANUAL ................................................................................................... 1 
 
IMPORTANT .................................................................................................................... 3 
Precautions on Electromagnetic Wave Interference ...... 3 
1. Possible Electromagnetic Wave Interference Caused   
by the Instrument  ............................................................. 3 
2. Possible Electromagnetic Wave Interference Affecting   
the Instrument  .................................................................. 3 
 
WARRANTY ON PRODUCT ............................................................................................ 5 
 
Installation, Relocation and After-sale Technical Service .......................................... 7 
 
Technical Seminars and Training for Customers ........................................................ 7 
 
OTHER PRECAUTIONS .................................................................................................. 8 
Handling of Chemicals and Samples  ................................... 8 
Notice on This Instruction Manual  ........................................ 8 
Trademark Acknowledgments  .............................................. 8 
 
SAFETY SUMMARY.................................................................................... SAFETY-1 
PRECAUTIONS ON SAFETY  ............................... SAFETY-1 
   General Safety Guidelines  ............................... SAFETY-1
 
Safety Notices on Fluorescence   
Spectrophotometer ................................................. SAFETY-3 
Electricity ............................................................... SAFETY-3 
Source of Heat/Flame  ........................................... SAFETY-3 
Data Backup  .......................................................... SAFETY-3 
Protection against Computer Viruses  .................... SAFETY-4 
Power Interruption  ................................................. SAFETY-4 
Turning On/Off Personal Computer  ....................... SAFETY-5 
 
 
FOREWORD...............................................................................................FOREWORD-1 
 
 
 
  
						

							 
- ii - 1.  FUNCTIONS AND SCREEN COMPOSITION ...................................................... 1-1 
1.1 Functions ............................................................. 1-1 
1.2  Installing the FL Solutions Program .................. 1-5 
1.3  Startup of FL Solutions Program ....................... 1-9 
1.4 Screen Composition ............................................ 1-13 
1.4.1  Basics of Window Operation  ..................... 1-13 
1.4.2  Kinds of Windows  ...................................... 1-17 
1.4.3  Data Processing Window  .......................... 1-18 
1.4.4  Explanation of Tool Buttons  ...................... 1-21 
1.5  Functions of Function Keys ............................... 1-26 
1.6  Explanation of Monitor Window ......................... 1-27 
1.7  Input of Spectrophotometer Serial No. .............. 1-30 
1.8 Window Setting .................................................... 1-31 
1.9  Saving a Data File ................................................ 1-39 
1.10  Entry of File Name for Saving ............................ 1-40 
1.11  Photometer Menu Commands ............................ 1-42 
1.12  Utility Menu Commands ...................................... 1-43 
1.13  Termination of FL Solutions Program ............... 1-44 
 
2. WAVELENGTH SCAN .......................................................................................... 2-1 
2.1  Flow of Operation ................................................ 2-1 
2.2  Creating an Analysis Method ............................. 2-2 
2.3  Defining Your Samples ....................................... 2-15 
2.4 Prescan ................................................................. 2-17 
2.5  Conducting a Wavelength Scan ......................... 2-18 
2.6 Data Printout ........................................................ 2-20 
 
3. TIME SCAN ........................................................................................................... 3-1 
3.1  Flow of Operation ................................................ 3-1 
3.2  Creating an Analysis Method ............................. 3-2 
3.3  Defining Your Samples ....................................... 3-14 
3.4  Conducting a Time Scan ..................................... 3-16 
3.5 Data Printout ........................................................ 3-18 
3.6  Calculating the Phosphorescence Life ............. 3-20 
 
4. PHOTOMETRY ..................................................................................................... 4-1 
4.1  Flow of Operation ................................................ 4-1 
4.2  Creating an Analysis Method ............................. 4-2 
4.3  Defining Your Samples ....................................... 4-21 
4.4 Conducting Measurement .................................. 4-22 
4.4.1  Manual Input of Standard Data  ................. 4-26 
4.4.2 Statistical Calculation ................................ 4-27 
4.5 Printing Data ........................................................ 4-28 
4.6  Remeasuring Standard Data and   
Sample Data ......................................................... 4-30 
4.7  Manual Input of Standard Data ........................... 4-31 
4.8  Measurement Using Saved   
Calibration Curve.................................................. 4-32  
						

							 
- iii 
-  5. 3-D SCAN................................................................................................................ 5-1 
5.1  Flow of Operation ................................................ 5-1 
5.2  Creating an Analysis Method ............................. 5-2 
5.3  Defining Your Samples ....................................... 5-13 
5.4 Conducting Measurement .................................. 5-14 
5.5  Displaying a Bird’s-Eye View ............................. 5-16 
5.6 Data Printout ........................................................ 5-17 
 
6. DATA PROCESSING ............................................................................................ 6-1 
6.1  Loading of Data ................................................... 6-1 
6.2 Spectrum Tracing ................................................ 6-3 
6.3 Scale Change ....................................................... 6-5 
6.4  Change of Graph Axis ......................................... 6-6 
6.5  Change of Spectral Line ..................................... 6-7 
6.6  Detection of Spectral Peaks ............................... 6-9 
6.7  Indication of Peak Wavelength on Spectrum .... 6-10 
6.8  Smoothing of Spectrum ...................................... 6-12 
6.9  Derivative Operation on Spectrum .................... 6-13 
6.10  Overlaid Display of Spectra ................................ 6-14 
6.11  Arithmetic Operation between Spectra ............. 6-17 
6.12 Area Calculation .................................................. 6-20 
6.13  Transfer of Data to Microsoft(R) Excel .............. 6-23 
6.14  Pasting of Spectra to Microsoft(R) Word .......... 6-25 
6.15  Conversion of Data to ASCII Text File ............... 6-27 
6.16  Renaming Your Sample ...................................... 6-29 
6.17  Printing of Wavelength and   
Photometric Values under Display .................... 6-30 
6.18  Reference to Spectrum Measured in   
Photometry Mode ................................................ 6-33 
 
7.  MORE CONVENIENT OPERATING METHODS (APPLICATION PART) ............ 7-1 
7.1  For Always Starting Up with   
Same Analysis Method ....................................... 7-1 
7.2  Online/Offline Setting of Photometer ................. 7-3 
7.3  Check of Data File Information ........................... 7-4 
7.4  Saving of Analysis Method ................................. 7-7 
7.5 Even Log .............................................................. 7-8 
7.6  Check of Program Version ................................. 7-10 
7.7  Saving of Sample Table ...................................... 7-11 
7.8  Change of Window Display ................................ 7-12 
7.9  Check of Photometric Value at   
Specified Wavelength........................................... 7-15 
7.10  Collective Printing of Files ................................. 7-16  
						

							 
- iv 
-  8.    MEASUREMENT USING OPTIONAL ACCESSORIES ........................................ 8-1 
8.1  Use of Sample Sipper .......................................... 8-1 
8.1.1  When Not Using a Sample Table  .............. 8-1 
8.1.2  When Using a Sample Table  .................... 8-4 
8.1.3  In Wavelength Scan or   
Time Scan Mode  ....................................... 8-8 
8.2  Use of AS-3000 Intelligent Autosampler ........... 8-9 
8.2.1  When Not Using a Sample Table  .............. 8-9 
8.2.2  When Using a Sample Table  .................... 8-9 
8.3  For Analog Output ............................................... 8-10 
  
APPENDIX ................................................................................................................... A-1 
 
INDEX ................................................................................................................... INDEX-1  
						

							1 - 1 
1.    FUNCTIONS AND SCREEN COMPOSITION   
 
 
1.1  Functions  
 
In this program, the following measurement modes are available:   
• Wavelength scan   
• Time scan   
• Photometry  
• 3-D Scan   
(‘3-D Scan program’ (option) required for Model F-2500)   
 
Wavelength Scan 
(1)  Photometric value data can be printed at the specified 
wavelength intervals or at up to 12 specified wavelengths.     
 
(2)  The function keys provide functions unique to the 
wavelength scan mode.   
Measurement:  [F4], Stop:  [F6]  
 
(3)  Wavelength scan measurement can be repeated max. 99 
times.    And, a repetition period is settable.   
 
Time Scan 
(1)  Kinetics, or rate calculation will be performed.   
 
(2)  Photometric value data can be printed at the specified time 
intervals or at up to 12 specified time points. 
 
Photometry 
(1)  Quantification method is selectable among specified 
wavelength, peak area, peak height, peak ratio and 
derivative methods.    Up to 20 standards can be measured.   
A calibration curve is prepared by drawing polygonal lines 
through each point of standard concentrations or a 
regression calibration curve is prepared by least square fit.   
It is also possible to measure up to 20 standards and 
prepare a calibration curve based on an average value of 
standard concentrations.   
 
(2)  A calibration curve can be redrawn by re-measuring a 
specific standard.    In a calibration curve graph on the 
screen window, a value at each point can be read out 
using the cursor.   
 
1.1