Hitachi F 2500 Manual

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5.3  Defining Your Samples  
 
Samples must be defined before measurement.   
Click the     button.  The dialog box in Fig. 5-8 will appear.   
 
 
 
Fig. 5-8 
 
 
Input for Sample and Comments.    For automatic saving, put a 
check mark in the “Auto File” box and specify a file name.     
To specify a file name, click Select.  
5.3  
						

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5.4  Conducting Measurement  
 
(1)  There are two methods of starting the measurement.     
 
(a)  Select the Measure command from the 
Spectrophotometer menu.   
 
(b)  Click the     (measurement) button on the 
measurement toolbar.   
For halting the measurement midway, click the   
button on the toolbar.   
 
(2)  When automatic saving is set, data will be saved after 
completion of measurement.    Unless automatic saving is 
set, select the Save As command from the File menu and 
save data in file.    Upon opening its file, a data processing 
window will appear as in Fig. 5-9.   
 
 
 
 
 
 
 
Fig. 5-9    Data Processing Window (3-D Scan) 
 
 
(a) Contour lines  
From these contour lines, excitation and emission 
spectra can be read out.     
As the cursor is moved, the cursor-specified excitation 
and emission spectra are displayed in (b) and (c).       
 
(a) Contour  lines   (b) Excitation spectrum   (d) Peak  table   
(c) Emission spectrum   
5.4  Conducting Measurement  
						

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(b) Excitation spectrum  
An excitation spectrum at the cursor position in (a) is 
displayed.    Wavelength and photometric value can be 
read by selecting the trace command from the View 
menu or clicking the     button.   
 
(c) Emission spectrum  
An emission spectrum at the cursor position in (a) is 
displayed.    Wavelength and photometric value can be 
read by selecting the trace command from the View 
menu or clicking the     button.   
 
(d) Peak table  
Peak wavelength and peak height are indicated.     
For setting threshold and sensitivity values, click the   
     button.   
 
 
5.4  
						

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5.5    Displaying a Bird’s-Eye View   
 
3-D Scan data can be displayed in a bird’s-eye view.     
Select the Bird’s-eye view command from the View menu or click 
the          (bird’s-eye view) button on the Spectrum toolbar.   
 
 
 
Fig. 5-10 
 
 
Viewing direction is changeable by clicking          (from front),   
    (from rear),     (from left) or     (from right).   
Clicking          (contour line diagram) displays a contour line 
diagram.   
 
 
5.5    Displaying a Bird’s-Eye View  
						

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5.6  Data Printout  
 
Printout can be provided even when the function for automatic 
printout after measurement is not used.    The following two 
procedures are available.   
 
(1)  Select the Print command from the File menu.    Or click the   
        print button on the toolbar.    The field active on the 
data processing window (e.g., (a) in Fig. 5-9) will be printed.     
The contents of display upon clicking the Print Preview 
command in the File menu will be printed.   
 
 
 
Fig. 5-11 
 
 
 
5.6  
						

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(2)  Select the Report command in the Data menu.    Or click 
the     button on the toolbar.  The Print Preview window 
will appear, so click Print.    Select the items to be printed 
at the Report tab under Properties.   
 
 
 
Fig. 5-12 
5.6  Data Printout  
						

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6.  DATA PROCESSING  
 
 
This section explains the operation method for data processing 
after measurement.   
 
6.1  Loading of Data  
 
Load saved data as follows.     
Either click the          (Open) button or select the Open command 
from the File menu.    A window as in Fig. 6-1 will appear.     
 
 
 
Fig. 6-1 
 
 
Select the file type of data file in “Files of type” where file types 
are listed.    Specify a desired file and click Open, and the data 
file will open.     
The following types are included in “Files of type.” 
•  Wavelength scan files (*.fds)   
•  Time scan files (*.fdt) 
•  Photometry files (*.fdq)   
•  3-D Scan files (*.fd3)   
•  Method files (*.flm)   
•  Sample table files (*.fls)   
•  Report files (*.flr)   
6.1  
						

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•  Processed data files (*.fdp)   
•  F-4500 wavelength scan files (*.fds) 
•  F-4500 time scan files (*.fdt)   
•  F-4500 photometry files (*.fdq)   
•  F-4500 3-D Scan files (*.fcd)   
•  All files (*.*)   
 
The window (Fig. 6-1) has four tool buttons.    Table 6-1 lists the 
tool buttons and their functions.   
 
Table 6-1    File-Open Window Buttons 
 
Button Function  
 Displays a folder of one level higher.     
 
Creates a new folder.   
 Lists the file names included in selected 
folder.  
 
Displays file details such as file name, 
size and type.   
 
 
NOTE:  When the remaining memory capacity of PC in 
operation is very small (system resource is less than 
20%), the data processing window will not open.   
Either close the presently opened data processing 
window or restart the FL Solutions program.   
6.1  Loading of Data  
						

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6.2  Spectrum Tracing  
 
This function is used to read the photometric value at a certain 
wavelength on the measured spectrum.    First, bring the cursor 
onto the displayed spectrum, then right-click the mouse and a 
window as in Fig. 6-2 appears.   
 
 
 
Fig. 6-2 
 
 
Put a check mark before Trace or click the          button.    A line 
cursor appears on the spectrum.    The wavelength and 
photometric value at the cursor position are indicated at the 
location (a) in Fig. 6-3.     
 
 
 
Fig. 6-3 
 
 
(a) 
 
6.2  
						

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The line cursor can be moved in the following two ways.   
 
(1)  Movement using mouse   
 
Move the line cursor with the mouse to the position for 
readout.  
 
(2)  Movement using arrow keys on the keyboard   
 
Click the [→] or [←] key and the line cursor moves in the 
corresponding direction.   
6.2  Spectrum Tracing