Hitachi F 2500 Manual

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1.11  Photometer Menu Commands  
 
The following commands are available in the Spectrophotometer 
menu.  
• Set Wavelength   
• Zero Adjust   
• Auto Pre-scan   
• Shutter Open/Close   
 
(1) Set Wavelength  
 
This command sets the excitation/emission wavelength to a 
specified wavelength.   
Click the Set Wavelength command or button, and the 
dialog box shown below will come up.   
 
 
 
 
(2) Auto Pre-scan  
 
This command automatically searches for an optimum 
excitation/emission wavelength for the sample under 
measurement.    Other peaks than the fluorescence peak 
(such as peaks of excitation scattered light and Raman 
spectrum) are ignored.    On completion of automatic pre-
scanning, the optimum excitation/emission wavelength is 
indicated on the monitor window (in the current wavelength 
indication field detailed in 1.6).     
 
(3) Zero Adjust  
 
This command automatically closes the shutter and 
measures a zero point in detection.    When the zero point 
is changed, e.g., after the photomultiplier voltage is 
changed, automatic zero-point adjustment is carried out.   
 
(4) Shutter Open/Close  
 
This command is available for opening/closing the 
excitation beam shutter.    The current state is indicated on 
the right side of the monitor window.     
1.11  Photometer Menu Commands  
						

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1.12  Utility Menu Commands  
 
The Utility menu contains the following commands: 
• Instrumental Response   
• Analog Output Gain 
• EX Power Monitor   
• Sens. Check   
• Lamp Usage   
• Lamp Off (only with Model F-2500)   
 
(1) Instrumental Response  
 
When you use this function.    You need the Spectral 
correction accessories kit (P/N 251-0126).       
 
(2)  Analog Output Gain   
 
Use this command for setting a data value corresponding to 
1 V of analog output.    For details, refer to 8.4.   
 
(3)  EX Power Monitor   
 
Use this command for checking output of the monitor 
detector.    When the EX Power Monitor is turned on 
(clicked), monitor value data is displayed regardless of 
measurement condition.   
 
(4) Sens. Check  
 
Use this command for checking sensitivity.    You can check 
sensitivity/drift with this command.    For details, refer to the 
maintenance edition.   
 
(5) Lamp Usage  
 
Use this command for checking/resetting the usage period 
of the xenon lamp.    A cumulative turn-on time of the xenon 
lamp is indicated.    After lamp replacement, reset this 
indication.    For details, refer to the maintenance edition.   
 
(6)  Lamp Off (only with Model F-2500)   
 
Use this command for turning off the xenon lamp.   
For turning on the xenon lamp again, restarting the 
spectrophotometer is necessary.     
1.12  
						

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1.13    Termination of FL Solutions Program   
 
After completion of measurement, shut down the instrument in 
the following procedure.   
 
(1)  From the File (F) menu, select the Exit (X) command.   
 
 
 
Fig. 1-42 
 
 
Select Yes, and the FL Solutions program will then be 
terminated.   
 
(2)  Turn off the power switch of the spectrophotometer main 
unit.  
 
(3)  Click the Start button of the Windows 98 and select the 
Shut Down (U) item.    In the Shut Down Windows dialog 
box, select “Shut down” and click OK.  
 
(4)  Turn off power to the personal computer and the CRT 
display monitor.   
(At step (3), the personal computer may be turned off in 
some settings.)   
 
(5)  Turn off power to the printer.   
  
1.13    Termination of FL Solutions Program  
						

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2.  WAVELENGTH SCAN  
 
 
2.1  Flow of Operation  
 
This section explains the condition setting and operating method 
for wavelength scan.     
Measurement is made in the following procedure.   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Fig. 2-1    Operational Flow (wavelength scan)   
End 
Start 
Method setting 
Click  button.  
 Scan range 
 Data mode 
 Scan speed     
 
Sample name/comment input
Click  button.  
Pre-scan 
Measurement 
Data processing 
 Spectrum tracing 
 Peak detection, etc.   
Print  (See page 2-2.) 
(See page 2-15.) 
 
(See page 2-16.)   
 Click 
 button.  
(See page 2-16.)   
 Click 
 button.  
(Refer to Section 6.) 
Click 
 button.  
(See page 2-19.)     
 
 
 
2.1  
						

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2.2    Creating an Analysis Method   
 
Set the analytical conditions (method) prior to measurement.   
From the Edit menu, select the Method command or click the 
        button.    The dialog box shown in Fig. 2-2 will appear.   
 
 
 
Fig. 2-2  Analysis Method 
 
 
The following tabs are provided on the Analysis Method window 
for the wavelength scan mode.    These tabs are explained on 
the subsequent pages.     
 General 
 Instrument 
 Monitor 
 Processing 
 Report  
2.2    Creating an Analysis Method  
						

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(1) General  
 
The parameters shown in Fig. 2-3 are provided under the 
General tab.   
 
 
 
Fig. 2-3  General Tab 
 
 
(a) Measurement (mode)  
Select a measurement mode from among the four 
listed below.   
 Wavelength scan  
 Time scan  
 Photometry  
  3-D Scan (displayed when the ‘3-D Scan program’ 
(option) is installed in combination with Model   
F-2500)  
 
Here, select “Wavelength scan.”    For Photometry, 
Time scan and 3-D Scan, refer to Sections 3, 4 and 5.   
 
(b) Operator  
Enter an operator name.     
 
(c) Instrument  
The model of the connected instrument is indicated.   
 
(d)  Use sample table (Set measurement sample) 
Apply a check mark here when a sample table is used.     
2.2  
						

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(e) Sampling  
 Standard  
When sample is exchanged manually.   
 
(f) Comments  
Enter a description or notes on measuring conditions.   
 
(g) Load  
Click this button to read out saved analytical conditions.   
The file opening window appears upon clicking, so 
select an analytical conditions file.   
 
(h) Save  
By clicking this button after setting the parameters for 
all five tabs, the analytical conditions are saved.   
Clicking this button overwrites parameters on the 
existing ones.   
 
(i) Save As  
Click this button for renaming the set conditions for 
saving.    The “Save As” window will appear, so enter a 
file name.   
 
(2) Instrument  
 
When you click the Instrument tab, the window in Fig. 2-4 
appears.  
 
 
 
Fig. 2-4  Instrument Tab 
2.2    Creating an Analysis Method  
						

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(a) Scan mode  
Select one of the following scan modes.    In case of 
Synchronous, excitation wavelength appears on the 
abscissa of the graph.     
 Excitation  
 Emission  
 Synchronous  
 
(b) Data mode  
Select one of the following data modes.   
The Luminescence mode is for measuring 
luminescence in the biological/chemical field and only 
the sample side signal is acquired without performing 
ratio photometry.    The Phosphorescence mode is for 
acquiring only phosphorescence after extinction of 
excitation beam while turning a chopper on the 
excitation side.   
 Fluorescence  
 Luminescence  
  Phosphorescence (with Model F-4500)   
 
(c) EM WL  
This is the start wavelength for wavelength scan on the 
excitation side.    Input the shorter wavelength side of 
measuring wavelength range.   
 
Model Input Range   
F-4500 200 to 900 nm or 200 to 890 nm in 
Synchronous scan mode   
F-2500 220 to 800 nm or 220 to 790 nm in 
Synchronous scan mode   
 
 
(d)  EX Start WL   
This is the start wavelength for wavelength scan on the 
excitation side.    Input the shorter wavelength side of 
measuring wavelength range.   
 
Model Input Range  
F-4500  200 to 890 nm   
F-2500  220 to 790 nm   
 
2.2  
						

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(e)  EX End WL   
This is the end wavelength for wavelength scan on the 
excitation side.    Input the longer wavelength side of 
measuring wavelength range.   
 
Model Input Range  
F-4500  210 to 900 nm   
F-2500  230 to 800 nm   
 
 
(f) EX WL  
An excitation wavelength can be input.    It is the end 
wavelength for wavelength scan on the excitation side.   
Input the longer wavelength side of measuring 
wavelength range.   
 
Model Input Range  
F-4500  200 to 900 nm   
F-2500  220 to 800 nm   
 
 
(g)  EM Start WL   
This is the start wavelength for wavelength scan on the 
emission side.    Input the shorter wavelength side of 
measuring wavelength range.     
 
Model Input Range  
F-4500  200 to 890 nm   
F-2500  220 to 790 nm   
 
 
(h)  EM End WL   
This is the end wavelength for wavelength scan on the 
emission side.    Input the longer wavelength side of 
measuring wavelength range.     
 
Model Input Range  
F-4500  210 to 900 nm   
F-2500  230 to 800 nm   
 
2.2    Creating an Analysis Method  
						

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(i) Scan speed  
Set a wavelength scan speed.  (Unit:  nm/min)  
 
F-4500 F-2500  
30000 3000 
12000 1500 
2400 300 
1200 60 
300 15 
60  
15  
 
 
For the Phosphorescence data mode, select one of the 
following values.   
 240 nm/min  
 60 nm/min 
 15 nm/min 
 
(j) Delay  
After pressing the Measure button, measurement is 
started following the delay time set here.    It is used for 
temperature stabilization, etc.    In repeat measurement, 
it is the time until the start of the first measurement.   
The delay time is invalid for the second and 
subsequent measurements.   
Input range:    0 to 9999 s   
 
(k) EX Slit  
Select a slit width for the excitation side.    Unit:    nm   
 
F-4500 F-2500  
1.0 2.5 
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
2.2