Hitachi F 2500 Manual

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(l) EM Slit  
Select a slit width for the emission side.    Unit:    nm   
 
F-4500 F-2500  
1.0 2.5  
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
(m) PMT Voltage  
A function for controlling the voltage of the 
photomultiplier detector (photomultiplier tube).     
Select one of the following values.    Changing from 
400 V to 700 V increases the data value about two 
digits and changing from 700 V to 950 V increases it 
about 1 digit.     
  950 V (only with F-4500)   
 700 V  
 400 V 
 
(n) Response  
Select a response time from the following.   
 
F-4500 F-2500  
0.0004 0.04 
0.01 0.08 
0.05 0.4 
0.1 2 
0.5 8 
2.0 Auto 
8.0  
Auto  
 
 
By selecting Auto, a response time is set automatically 
according to scan speed.    Select Auto usually.   
 
2.2    Creating an Analysis Method  
						

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(o) Corrected spectra  
A function for determining the spectrum inherent to a 
sample by correcting the photometer wavelength 
characteristic using the saved instrument parameters, 
following measurement with the instrument parameters 
for photometer control.    If there are no instrument 
parameters for the wavelength range to be measured, 
only the wavelength range that allows correction is 
scanned.    And if there is no wavelength area 
measurable, an error will occur.     
For using this function, a Spectra Correction Parts Set 
(P/N 251-0126) is required.    The Instrument 
Response command is provided in the Utility menu for 
operating this function.     
When this setting is at ON, “Corrected spectra” 
appears on the monitor window.    (See Fig. 1-26 in 
1.6.)  
 
(p)  Shutter control (only with F-4500)   
The shutter can be automatically closed in other than 
measurement for suppressing sample deterioration 
due to the energy of excitation beam and opened when 
measurement starts.    When you put a check mark at 
the head, the shutter will close and open at start of 
measurement.    The shutter will close again when 
measuring wavelength begins returning to the start 
wavelength after measurement in the wavelength scan 
mode.   
 
(q) Replicates  
Set the number of repeat measurements.    Input range 
is 1 to 99.    An average spectrum can also be 
determined in repeat measurement.    Setting is 
available on the Processing tab.   
 
(r) Cycle time  
Set a repetition interval.    Input range is 0.0 to   
180.0 min.   
2.2  
						

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(3) Monitor  
 
When selecting the Monitor tab, the window in Fig. 2-5 
appears.   
 
 
 
Fig. 2-5  Monitor Tab 
 
 
Assign the following parameters.   
 
(a)  Y-Axis Max (maximum)   
Input a maximum value for Y-axis on the monitor 
window.  
 
(b)  Y-Axis Min (minimum)   
Input a minimum value for Y-axis on the monitor 
window.  
 
(c)  Open data processing window after data acquisition   
Select whether or not to conduct data processing after 
sample measurement.    When selected (check mark is 
applied), an icon is displayed for the data processing 
window at the end of measurement.    And by opening 
this icon, data processing such as peak detection is 
available.  
 
2.2    Creating an Analysis Method  
						

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NOTE:  When the remaining memory capacity of PC 
in operation is very small (system resource 
is less than 20%), the data processing 
window will not open even if this item is 
turned ON.    Either close the presently 
opened data processing window or restart 
the FL Solutions program.     
 
(d)  Print report after data acquisition   
Select whether or not to print a report after sample 
measurement.    When selected (check mark is 
applied), printing will be made automatically after 
measurement.    The items set under the Report tab 
will be printed.    When this item is not selected, data 
processing can be made by reading out a file via File - 
Open.  
 
(e) Overlay  
Spectra can be overlaid in the monitor window.     
This setting cannot be selected for repeat 
measurement.  
 
(4) Processing  
 
On selection of the Processing tab, the window in Fig. 2-6 
will be displayed.   
 
 
 
Fig. 2-6  Processing Tab 
 
2.2  
						

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(a)  CAT (Computing for Averaging Transient)   
Used for obtaining an average spectrum in repeat 
measurement.    This is especially effective for 
measuring samples with which the emission intensity 
varies with time.   
 
(b) Processing choices  
A list of data processing (Savitsky-Golay, Mean 
smooth, Median smooth, Derivative) is shown.     
Select a data processing item, and click the rightward-
pointing arrow key between the Processing choices 
and Processing steps display fields.    Then, the 
selected method appears in the Processing steps field.   
 
(c) Processing steps  
The processing sequence is displayed.    To delete a 
processing method, first select the method, and then 
click the leftward-pointing arrow key between the 
Processing choices and Processing steps display 
fields.    Then, the selected method disappears from 
the Processing steps field.   
Also, in case you want to carry out any smoothing in 
Processing steps, double-click the relevant item and 
the smoothing parameters (order, data points, etc.) can 
be changed.   
 
(d) Peak Finding  
Select a peak detection method indicated in the data 
processing window.   
 
(e) Threshold  
Set a detection limit for the photometric value axis of 
peak and valley.    Peaks or valleys below the setting 
will not be detected.    Input range is 0.001 to 1000.     
 
(f) Sensitivity  
Select the number of data points in the horizontal axis 
direction (abscissa).    Select a sensitivity of “1” for 
detecting sharp peaks.    Or select “8” for detecting 
broad peaks.   
 1  
 2 
 4 
 8 
 
2.2    Creating an Analysis Method  
						

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(5) Report  
 
Select the Report tab, and the window in Fig. 2-7 will 
appear.  
 
 
 
Fig. 2-7  Report Tab 
 
 
This window is used to select an output method and items 
of measurement result.     
 
(a) Output  
1) Print Report  
A report will be printed.    For printing method, 
refer to 2.6.   
2)  Use Microsoft® Excel   
Data will be transferred to Microsoft® Excel.     
For transfer method, refer to 5.13.   
3)  Use print generator sheet 
Select this method for using the report generator 
(option).    For usage, refer to the instruction 
manual of the report generator.   
 
(b) Orientation  
Selectable when Report is chosen for Print Method.   
Select a printing orientation.    Either Portrait or 
Landscape orientation is selectable.    When the 
Landscape orientation is selected, date and method 
can be printed.   
2.2  
						

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(c)  Printable (transferable) items   
1) Include date  
2) Include method  
3) Include graph  
4)  Include data listing   
 Constant  
Data will be printed at fixed intervals between 2 
wavelength points.    Input Data interval, Start 
wavelength and End wavelength.     
 Select data 
Data at the specified wavelength will be printed.   
Up to 12 wavelengths can be specified.   
5)  Include peak table 
Select output items in the peak table.   
  Peak WL/Peak data   
  Start WL/End WL   
  Valley WL/Valley data   
 Peak Area  
 
(d) Printer Font  
Click this button for changing the font to be used in the 
report.  
2.2    Creating an Analysis Method  
						

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2.3  Defining Your Samples  
 
When “Use Sample Table” has been set for the Analysis Method, 
the samples must be defined before measurement.   
 
(1)  Open the monitor window and click the          button.   
 
(2)  When the Sample Table window in Fig. 2-8 appears, fill in 
the blank section.    For instance, if there are five samples, 
click the Insert button five times.   
 
 
 
Fig. 2-8    Sample Table Window 
 
 
(3)  Input the Sample, Comments and File Name for each 
sample.    The sample names input here appear on the 
monitor window also.    And the sample names input here 
are not changeable.     
Maximum number of characters to be input   
Sample : 20 characters  
Comments :  255 characters   
 
 
2.3  
						

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(4)  When input is finished, check the entries and then click the 
OK button.   
If a sample table is not used, then click the          button.   
The window in Fig. 2-9 will appear.    Make entries for 
Sample and Comment.    For automatic saving, put a check 
mark in the “Auto File” box and specify a file name for 
saving.  
 
 
 
Fig. 2-9 
 
2.3  Defining Your Samples  
						

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2.4  Prescan  
 
Select Pre-scan command from the Spectrophotometer menu or 
click the     (pre-scan) button.  
This function executes high-speed scan under the set measuring 
conditions, detects the peak wavelength and moves the system 
to that wavelength.    Also, the upper limit photometric value is 
automatically set so that the peak-wavelength value becomes 
approximately 70% of that upper limit value.     
Scan ranges are as follows.   
 
Excitation spectra  :  Start wavelength to emission 
wavelength - 20 nm or shorter 
wavelength side of end wavelength   
 
Emission spectra  :  Excitation wavelength or longer 
wavelength side of start wavelength + 
20 nm to end wavelength   
 
Synchronous spectra :  Start wavelength to end wavelength   
 
After prescan, the upper limit photometric value is automatically 
set.   
2.4