Hitachi F 2500 Manual

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(3)  A calibration curve can be printed.   
A calibration curve can be drawn by inputting its factor 
value.  
 
(4)  Measurement data and a calibration curve are displayed 
on the same screen window.    It is therefore possible to 
carry out measurement while observing the calibration 
curve.  
 
(5)  According to the coefficient of determination calculated 
automatically, judgment can be made on a degree of 
matching between standard values and a calibration curve.     
Two/three-wavelength calculation is permitted in 
preparation of a calibration curve.   
 
(6)  Statistical calculation is possible.   
Mean value of measured data and standard deviation (SD) 
can be calculated.   
 
(7)  Upper/lower limit judgment will be performed.   
 
(8)  Spectrum display under photometry   
In the data measured by a quatification method; peak 
height, peak area, peak ratio or derivative, a spectrum due 
to wavelength scan is displayable.   
 
3-D Scan 
(1)  Spectral calculation can be performed using the measured 
data.  
 
(2)  A peak table can be displayed.   
 
(3)  A bird’s-eye view/synchronous spectrum can be displayed 
and printed.   
 
(4)  Printing conditions are selectable.   
In use of the DDE function, the wavelength interval and 
start/end wavelengths of data list can be selected.   
 
1.1  Functions  
						

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Secondary Processing of Data 
Measured data can be saved, and a variety of processings can 
be carried out on the saved data.   
 
(1)  Automatic reading of data (spectrum tracing)   
By placing the cursor on the currently displayed spectral 
graph, a photometric value at the cursor position can be 
read out.   
 
(2) Peak detection  
Peaks and valleys of the currently displayed spectrum are 
detected automatically.   
 
(3)  Change of scale   
A measured spectrum can be enlarged or reduced by 
changing the ordinate and abscissa scales.   
 
(4) Smoothing  
A measured spectrum can be smoothed for elimination of 
noise components.   
 
(5) Derivative  
Spectral derivative is obtainable.    It is permitted to specify 
an order of derivative, that of smoothing and a count value 
of data.   
 
(6) Spectrum calculation  
Fundamental arithmetic operations can be made between 
spectra or a spectrum can be multiplied with a factor.   
 
(7) Area  
The area value of the specified peak will be calculated.   
The average area value is also calculable among peaks.   
 
(8) File conversion  
Measurement data can be converted into an ASCII text file 
(*.txt) or JCAMP-DX file (*.dx).    It is also possible to save 
a spectrum and calibration curve graph in a Windows meta 
file (*.wmf).   
 
(9)  Display/print font setting   
The font, style and size of characters to be displayed and 
printed can be changed as desired.   
 
1.1  
						

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(10) Data acquisition  
Photometric value data on the data processing window and 
monitor window can be acquired.    Acquisition is allowed 
within 100 data.     
 
(11)  Printing items collectively specifiable   
Printing items on overlaid spectra can be specified 
collectively.  
 
(12)  Specification of printing items   
Items in a peak table can be selected and printed.   
 
(13)  Portrait or Landscape printing settable   
Printing can be made Portrait or Landscape.     
 
(14)  Collective spectrum printing   
Printing can be carried out just by specifying a file.   
 
(15)  Selection of spectrum to be displayed   
The spectrum to be displayed for overlaying is selectable.   
 
(16)  Automatic saving mode ON/OFF settable   
ON/OFF can be set in the sample name/comment input 
window.   
Automatic saving is effective in all the modes that use a 
sample table.   
 
(17)  Data in each mode can be transferred to Microsoft Excel.   
(This is valid when Microsoft Excel 97 has been installed 
in PC.)   
 
(18)  After copying spectral data into commercial software such 
as Microsoft Word, its scale, line type, color, etc. are 
changeable on the software.   
 
(19)  The report generator (separately available, P/N 250-0455) 
can be used.   
 
1.1  Functions  
						

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1.2    Installing the FL Solutions Program   
 
For using the FL Solutions program, it must be installed using 
the attached system disks.     
Installation of the FL Solutions program is required when using it 
at the first time (not required later for each operation).    In the 
following installation session, it is assumed that: 
•  The floppy disk drive is assigned as drive A. 
•  The destination of installation is assigned as drive C.   
 
(1)  Using the mouse, click the Start button of the Windows 98 
and select Settings - Control Panel.  The Control Panel 
window will then appear as shown in Fig. 1-1.   
 
 
Fig. 1-1 
 
 
(2)  In the Control Panel window, double-click the Add/Remove 
Programs icon.    The Add/Remove Programs dialog box 
will then appear as shown in Fig. 1-2.    In this dialog box, 
click Install.  
 
 
Fig. 1-2 
1.2  
						

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(3)  Then, when the dialog box shown in Fig. 1-3 appears, push 
in the first one of the attached system disks and click Next. 
 
 
Fig. 1-3 
 
 
(4)  Then, when the dialog shown in Fig. 1-4 appears, click 
Finish.  
 
 
Fig. 1-4 
 
 
Installation process will then begin.    Take steps according 
to the instruction messages.     
1.2    Installing the FL Solutions Program  
						

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(5)  On completion of the installation process, restart the 
personal computer.   
 
(6)  For startup of the FL Solutions program, refer to 1.3.   
 
NOTES:  1.  Monitor resolution must be set at 800 × 600 at 
minimum.    Otherwise, each window of the FL 
Solutions program may not be displayed fully.   
2.  When the large font size of display is selected, 
words may not be indicated normally on each 
window.    In this case, font size should be changed 
to the small one.    For change, select Control 
Panel - Display Properties - Settings - Font Size.   
 
1.2  
						

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Reference:   
Re-installation of FL Solutions Program   
 
For re-installing this program, remove it first and then install it in 
the following procedure.   
Select Start - Settings - Control Panel.    In the Control Panel 
window, double-click the Add/Remove Programs icon, and the 
dialog box shown in Fig. 1-5 will then appear.   
 
 
 
Fig. 1-5 
 
 
In this dialog box, select “FL Solutions” and click the Add/ 
Remove button.    When it is removed, click OK.  
Thereafter, install the FL Solutions program again.     
1.2    Installing the FL Solutions Program  
						

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1.3    Startup of FL Solutions Program   
 
To start up the FL Solutions program, take the following 
procedure.  
 
(1)  Connect the communication cable (standard accessory) 
between the fluorescence spectrophotometer and the 
personal computer.    Then, turn on power to the 
spectrophotometer and the computer in this order.     
 
(2)  The Windows 98 starts.   
 
(3)  Click the Start button.    Point to Programs and Hitachi 
Applications and select FL Solutions.    (See Fig. 1-6.)     
Or double-click the FL Solutions program icon.     
If the FL Solutions program is set as a startup application 
in the Windows 98, the program automatically starts when 
turning on power supply of the personal computer.     
This setting can be selected at installation of the program.     
 
 
 
 
 
Fig. 1-6 
Icon of FL Solutions program   
1.3  
						

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(4)  The fluorescence spectrophotometer connected to the 
communication COM port of computer is now recognized 
and the window shown in Fig. 1-7 is displayed.    Then, the 
spectrophotometer is initialized.     
 
 
 
Fig. 1-7 
 
 
(5)  A gauge of initialization is displayed as shown in Fig. 1-8, 
so its progress can be visually checked.   
 
 
 
Fig. 1-8 
1.3    Startup of FL Solutions Program  
						

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NOTE:  If the spectrophotometer is not connected with 
the COM port or its power is not turned on, the 
monitor window (“F-2500 Fluorescence 
Spectrophotometer on COM1” indicated on its 
title bar) is not presented.    In this status, 
functions for other than measurement are usable.   
For measurement, first confirm connection of the 
cable, and then turn on power supply of the 
spectrophotometer.  Next, select the 
Instruments (I) command from the Tools (T) 
menu.    When selecting and connecting the 
spectrophotometer, the window shown in Fig. 1-8 
is presented. 
 
In execution of initialization, self-diagnosis is performed for 
the following items:   
• ROM 
• RAM  
• Wavelength drive system   
• Xenon lamp turn-on   
• EEPROM  
• Slit mechanism   
• Chopper  
• Backup battery voltage (only with Model F-4500)   
• Contents of wavelength and slit calibration values   
 
If an abnormality is found in the self-diagnosis, the relevant 
error message will be displayed.    In this case, a corrective 
measure should be taken, referring to 4.7 of the 
maintenance edition.   
If necessary, notify our local repair service office.   
 
 
 
Fig. 1-9 
 
 
1.3