Hitachi F 2500 Manual
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6 - 15 (2) Open a file to be overlaid to display its data. Fig. 6-15 Drag the blue profile of the displayed spectrum so that the mouse pointer comes onto the target spectrum. When the mouse pointer changes as shown in Fig. 6-16, drop the blue profile. Fig. 6-16 (3) Open a file as in (2) above. Click the spectrum to be overlaid and press Ctrl+C on the keyboard or select the Copy (c) command from the Edit (E) menu. Then click the target spectrum and paste the first spectrum on it by Ctrl+V. The spectra are now overlaid. 6.10
6 - 16 (a) Deletion from Overlaid Display Select the Overlay command from the Processing menu. In case you want to delete the spectrum having sample name “Filter 02,” select the sample name and click Delete. Fig. 6-17 (b) Display of Selected Spectrum Alone from Overlaid Spectra Click (Display spectrum) on the Spectrum toolbar. Fig. 6-18 Click the spectrum not to be displayed. Click OK. In this case, spectra Nos. 1 and 2 will be displayed. Printout of this data covers not the spectra, but the sample names, analytical conditions, etc. After file saving in the current state, 4 spectra will be displayed upon loading the file again. To be displayed Not to be displayed 6.10 Overlaid Display of Spectra
6 - 17 6.11 Arithmetic Operation between Spectra Shown here is an example of obtaining the difference between two overlaid spectra. Display the spectrum for arithmetic operation. Now, click the Arithmetic (A) command on the Data (D) menu or click the (spectral calculation) on the Processing toolbar. A window as in Fig. 6-19 will open. Fig. 6-19 (1) The sample name of the file open in the window (file name in parentheses) is indicated. Select the file for arithmetic operation. (2) The spectrum selected in (1) is displayed here. (3) A factor for the spectrum selected in (1) is settable. For multiplying the spectrum by a factor, click Calculate here. (4) Apply a check mark here for arithmetic operation between two spectra. As a result, the window as in Fig. 6-20 appears. (2) Spectrum (1) Sample name (3) Factor input (4) Spectral calculation ON/OFF 6.11
6 - 18 Fig. 6-20 (5) Another sample name (file name in parentheses) is indicated. Select the file for arithmetic operation. (6) The spectrum selected in (5) is displayed. (7) Set the factor for the spectrum selected in (5). (8) Select a mathematical operator for the two spectra. +: Addition, -: Subtraction, *: Multiplication, /: Division After setting, click Calculate. The result of arithmetic operation is displayed as shown in Fig. 6-21. (6) Spectrum (5) Sample name (7) Factor input (8) Mathematical operator 6.11 Arithmetic Operation between Spectra
6 - 19 Fig. 6-21 After Arithmetic Operation Click Save As, and the result of calculation can be saved in file. Result of arithmetic operation 6.11
6 - 20 6.12 Area Calculation An area value is obtainable by integration in the specified range of the presently displayed spectrum. Select the Integration (I) command from the Data (D) menu or click the (area calculation) button on the Processing toolbar. The window as in Fig. 6-22 will appear. Fig. 6-22 Click Start and input the range for area calculation. Or move and right-click the cursor, and a value will be entered. In the same way, enter the end wavelength. After entry, click Calculate. The result will be displayed. For printout, click Print. In order to print a spectrum and the result of calculation, apply a check mark to “With spectrum record” and click Print. When clicking the OK button after calculation, its result will be saved. Operation will be reset by closing the data processing window or clicking the button on the Processing toolbar. Clicking Cancel closes the window. 6.12 Area Calculation
6 - 21 For scale expansion, specify the area to be expanded with the mouse. Draw a rectangle with the mouse button held down. Fig. 6-23 Then, release the button, and the specified area will be expanded as shown in Fig. 6-24. Fig. 6-24 Double clicking the right mouse button returns the scale to the original one. 6.12
6 - 22 The principle of calculation is shown below. Fig.6-25 The elements (D 1 to Dn) of a spectrum are subjected to the following calculation for obtaining the area value. S3 = {D 2 + D3 + … Dn-2 + Dn-1 + D1 + Dn}/2∗∆λ S2 = (D 1 + Dn) ∗ {∆λ∗ (n-1)} (D 1 + Dn) ∗ (Section start wavelength (WL1)) – (Section end wavelength (WL 2)) S1 = S3 – S2 D 1 : Spectral data at section start wavelength D n : Spectral data at section end wavelength ∆λ : Sampling interval In the time scan mode, change as follows. Start wavelength (WL 1) → end time (T2) End wavelength (WL 2) → start time (T1) 6.12 Area Calculation Section start wavelength Section end wavelength Specified range Dn D n - 1 D2 D 1 ∆ 2 WL 1 S2 S 1 WL 2WL1 Sb S a WL 2 S c S d
6 - 23 6.13 Transfer of Data to Microsoft(R) Excel Measured data can be transferred to a worksheet of Microsoft(R) Excel. Procedure is given below. (1) Click the (properties) button and select the Report tab. A window as in Fig. 6-26 will open. In Print Method, select “Use Microsoft(R) Excel.” Also select the data to be transferred (peak table and data list in case of wavelength scan). Then, click OK. Fig. 6-26 (2) Click the (Report) button and the Microsoft(R) Excel will automatically start to read the data. The subsequent procedure concerns the Microsoft(R) Excel operation. Fig. 6-27 6.13
6 - 24 (3) Data can be saved with extension code xls of Microsoft® Excel. Select File - Save Copy As and enter a file name. Fig. 6-28 (4) Upon closing the Microsoft® Excel, the window in Fig. 6-29 appears. Now close this window. The original data processing window will appear again. Fig. 6-29 6.13 Transfer of Data to Microsoft(R) Excel