Hitachi F 2500 Manual

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(1) Display Tab  
 
Items to be displayed when opening a saved data file or 
data immediately after measurement can be set here.     
Set the number of data columns to be displayed.     
The items other than the number of display columns to be 
set here can also be set by a tool button or View menu after 
measurement.  
 
 
 
Fig. 1-31  Display Tab 
 
 
(a) Trace  
Put a check mark in this box, and spectrum can be 
traced when the data processing window is open.   
 
(b) Ruled line  
The contents of monitor and data processing windows 
can be displayed with ruled lines. 
1) Wide  
2) Intermediate  
3) Narrow  
 
(c) Wavelength scan  
Items to be displayed when opening the data 
processing window in the wavelength scan mode can 
be set.   
1)  Show graph only   
2)  Show graph plus peak table   
1.8  Window Setting  
						

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(d) Time scan  
Items to be displayed when opening the data 
processing window in the time scan mode can be set.   
1)  Show graph only 
2)  Show graph plus kinetics data   
3)  Show graph plus peak table   
 
(e)  3-dimensional (effective for Model F-2500 when the   
‘3-D Scan program’ (option) is installed) 
Items to be displayed when opening the data 
processing window in the time scan mode can be set.   
1)  Show contour plus EXEM spectrum 
2)  Show contour, EXEM spectrum plus peak table 
 
(f)  Intracellular calcium (effective when the ‘intracellular 
calcium measurement program’ (option) is installed)   
Items to be displayed when opening the data 
processing window in the intracellular calcium mode 
can be set.   
1)  Show graph only 
2)  Show graph plus kinetics data 
3)  Show graph plus average data   
4)  Show graph plus markers   
 
(g)  Intracellular calcium spectrum (effective when the 
‘intracellular calcium measurement program’ (option) is 
installed)  
Items to be displayed when opening the data 
processing window in the intracellular calcium mode 
can be set.   
1)  Show fluorescence only 
2)  Show fluorescence plus ratio   
3)  Show fluorescence plus conc.   
4)  Show fluorescence, ratio plus conc. 
 
(h)  Fluorescence polarization (effective when the 
‘fluorescence polarization measurement program’ 
(option) is installed)   
Items to be displayed when opening the data 
processing window in the fluorescence polarization 
mode can be set.   
1)  Show graph only   
2)  Show graph plus kinetic data 
3)  Show graph plus markers 
1.8  
						

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(i)  Fluorescence polarization spectrum (effective when 
the ‘fluorescence polarization measurement program’ 
(option) is installed)   
Items to be displayed when opening the data 
processing window in the fluorescence polarization 
mode can be set.   
1)  Show fluorescence only 
2)  Show fluorescence plus polarization   
3)  Show fluorescence plus anisotropy     
4)  Show fluorescence, polarization plus anisotropy   
 
(2) Fonts Tab  
 
Display font, character size, etc. can be set.   
Printer font, character size, etc. are also settable.   
 
 
 
Fig. 1-32  Fonts Tab 
 
 
(a) Display Font  
Font, character size, etc. in the spectrum area under 
display can be set.   
 
1.8  Window Setting  
						

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(b) Printer Font  
Printer font is settable.   
Clicking Select (S) presents the following dialog box.   
 
 
 
Fig. 1-33  Font Specification 
 
 
Set Font, Font style and Size.   
 
(3) Processing Tab  
 
 
 
Fig. 1-34  Processing Tab 
1.8  
						

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(a)  Area integration method   
1) Rectangular  
2) Trapezoid 
3) Romberg 
 
(b) Calibration  
A calibration curve formula to be used for the 
Photometry mode can be set.   
1)  Data = f(Conc)   
2)  Conc = f(Data) 
 
(c) Peak table  
Inclusion of area calculation item in the peak table can 
be selected or avoided.   
 
(d) Smooth  
The initial values of data processing function 
parameters can be set.    However, this setting will not 
be reflected on the parameters of Analysis Method 
window.  
 
(e) Derivative  
The parameters of smoothing in an analysis method or 
to be carried out after measurement can be set.   
 
(4) Colors Tab  
 
The color of the spectrum to be overlaid is settable here.   
Color 1 (bottom) is the color of the first spectrum.   
 
 
Fig. 1-35  Colors Tab 
1.8  Window Setting  
						

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Click Select.    The dialog box shown in Fig. 1-36 is then 
presented.    A basic color or custom color is selectable.     
If there is no basic color you want, click Define Custom 
Colors (D).   
 
 
 
Fig. 1-36  Color Setting 
 
 
The dialog box shown in Fig. 1-37 will appear.    Select hue, 
saturation, luminance, etc. and click Add to Custom 
Colors (A).    Your color is then added to the custom colors.   
So select it and click OK.  
 
 
 
Fig. 1-37    Addition of Color 
1.8  
						

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(4) Startup Tab  
 
Analysis method to be taken upon startup of the FL 
Solutions program is settable here.   
 
 
 
Fig. 1-38  Startup 
 
 
(a)  Restore last method used   
The method used last will be brought in.     
 
(b)  Auto Parameter File 
The instrument will start with the fixed method brought 
in.    Click the model name of your instrument.   
 
 
 
Fig. 1-39 
 
 
Specify a file and click Open (O).  
1.8  Window Setting  
						

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1.9    Saving a Data File   
 
Data can or cannot be saved depending on setting.    So perform 
setting with reference to the following table.    Setting is any of 
patterns ① to ⑤ is recommended.  
 
Setting “Use sample table” 
ON/OFF Sample Name/Comment Input   
(“Use sample table” OFF) 
Automatic saving  ON  OFF 
Analysis Method - Monitor tab 
 
“Open data processing 
window after data acquisition” ON OFF ON OFF ON  OFF 
Display ○ ☓ ○ ☓ ○ ☓ 
Automatic saving ○ ○ ○ ○   
Result 
Manual saving     Possible Impossible
Pattern ① ② ③ ④ ⑤ ⑥ 
 
 
Read ○ and ☓  symbols in the Result - Display row as follows.   
○ :  Icons or data processing window is open immediately after 
measurement.  
☓ :  The data processing window is not open immediately after 
measurement.  In patterns ② and ④, data is saved.     
So the relevant data file can be opened later.   
1.9  
						

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1.10    Entry of File Name for Saving   
 
File name needs to be set before measurement.    Select the 
Sample Table (S) command in the Edit menu (E), and the dialog 
box shown in Fig. 1-40 will open.    Input a sample name and 
comment.    Then, bring the cursor to the file name column and 
click the button at (1).   
 
 
 
Fig. 1-40 
 
 
The dialog box shown in Fig. 1-41 will appear.    Enter your 
choice for “Save In” and specify “File Name.”   
 
 
 
Fig. 1-41 
 
(1)  1.10    Entry of File Name for Saving  
						

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After input, click Save.  
If the file name field remains blank, a file name such as 
default001.uds is automatically given.   
Once a measurement has been performed, you may perform the 
next ones with no change in setting (using the same sample 
table).    In this case, file names “Sample 001.uds”, “Sample 
002.uds” and so on will be given.    However, when there is no 
file name in a loaded analysis method file, file names will be 
given using the analysis method name as follows; Method .uds, 
Method 001.uds and Method 002.uds.     
Enterable number of characters   
Sample name :  20 characters 
Comment : 255 characters 
 
NOTE:  If the specified storage medium (such as floppy disk or 
MO) does not have an adequate free area for new 
storage, the relevant file will be saved in the FL 
Solutions folder.    This also occurs when the specified 
folder does not have an adequate free area.   
1.10