Hitachi F7000 Instruction Manual

3.2    Creating an Analysis Method 
3 - 6 
3 EX WL   
Enter an excitation wavelength.   
4 EM WL   
Enter an emission wavelength.   
5 Time unit   
Set a time unit.   
6 Scan time   
Set a wavelength scan speed.   
For the relationship between scan time and sampling interval, refer 
to Appendix H.   
7 Delay  
After pressing the Measure button, measurement is started 
following the delay time set here.    It is used for temperature 
stabilization, etc.    In repeat measurement, it is the time until the...

3.2.2 
3 - 7 
14 Replicates  
Set the number of repeat measurements.     
15 Cycle time   
Set a repetition interval.   
16 Stopped Flow   
When a check mark is put in this box, the stopped flow mode is set.   
In this case, when you click the measurement start button, the 
system waits until an external start signal comes in.     
Then, measurement starts.   
 
  

3.2    Creating an Analysis Method 
3 - 8 
When selecting the Monitor tab, the window in Fig. 3-5 appears.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 3-5  Monitor Tab 
 
 
Assign the following parameters.   
 
1 Y-Axis Max (maximum)   
Enter a maximum value for Y-axis on the monitor window.   
2 Y-Axis Min (minimum)   
Enter a minimum value for Y-axis on the monitor window.   
3 X-Axis Max (maximum)   
Enter a maximum value for X-axis on the monitor window.   
4...

3.2.3 
3 - 9 
5 Open data processing window after data acquisition   
Select whether or not to conduct data processing after sample 
measurement.    When selected (check mark is applied), an icon 
for this function (as shown in 
) is displayed.   
 
NOTE:  When the remaining memory capacity of PC in operation is 
very small (system resource is less than 20%), the data 
processing window will not open even if this item is turned ON.   
Either close the presently opened data processing window or 
restart...

3.2    Creating an Analysis Method 
3 - 10 
On selection of the Processing tab, the window in Fig. 3-6 will be 
displayed.  
For the settable range of each parameter, refer to Appendix G.   
 
 
Fig. 3-6  Processing Tab  
 
 
1 Processing choices   
A list of data processing is displayed.    Select a data processing 
item, and click the Right arrow button (
).  Then, the selected 
method appears in the Processing steps field.    For example, 
select ‘Derivative’ and click 
, then ‘Derivative’ is...

3.2.4 
3 - 11 
2 Processing steps   
The processing sequence is displayed.    After measurement, data 
processing will be carried out in the order set here.   
How to Delete a Processing Method   
Select a processing method to be deleted and click the Left 
arrow button (
).    Then, the selected method disappears 
from the Processing steps field.   
 
Display and Change of Parameter   
When you click [+] in the Processing steps field, a parameter list 
is displayed.    Click a parameter name to be...

3.2    Creating an Analysis Method 
3 - 12 
5 Peak Finding:  Sensitivity  
Select the number of data points in the horizontal axis direction 
(abscissa).    For detecting sharp peaks, select a sensitivity value of 
“1.”    Or select “8” for detecting broad peaks.   
6 Kinetics:  Start time  
Enter a start time for kinetics (rate calculation).   
7 Kinetics:  End time  
Enter an end time for kinetics.   
8 Kinetics:  K-Factor  
Enter a K-factor for kinetics.   
 
  

3.2.5 
3 - 13 
Select the Report tab, and the window in Fig. 3-9 will appear.   
 
 
 
Fig. 3-9  Report Tab 
 
 
This window is used to select a printout method and items of 
measurement results.   
 
1 Output  
• Print Report   
A report will be printed.    For printing method, refer to 3.5.   
• Use Microsoft(R) Excel   
Data will be transferred to Microsoft(R) Excel.    For transferring 
method, refer to 7.13.   
• Use print generator sheet   
Select this method for using the report generator...

3.2    Creating an Analysis Method 
3 - 14 
3 Print items (printable/transferable items) 
• Include date   
The date and time of analysis can be printed.    Printing time will 
always be printed.   
• Include method   
• Include graph   
• Include data listing   
◊ Constant  
Data will be printed at fixed intervals between 2 wavelength 
points.    Make entries for Data interval, Data start and Data 
end.  
◊ Select data  
Data at the specified wavelength will be printed.    Up to 12 
time points can be...

3.3.1 
3 - 15 
3.3  Defining Your Samples  
 
When “Use sample table” is not selected in Analysis Method, a sample 
must be defined in the following procedure before measurement.   
 
(1) Click the 
  button.    The window in Fig. 3-10 will appear.   
 
 
 
Fig. 3-10   
 
 
(2)  Make entries for Sample name and Comment.   
Maximum number of enterable characters (half-size)   
Sample name :  40 characters   
Comment : 255 characters  
 
For automatic saving, put a check mark in the “Auto File” box to...