Hitachi F7000 Instruction Manual

2.2    Creating an Analysis Method 
2 - 4 
7 Load  
Click this button to read out saved analytical conditions.     
Upon clicking, the file opening window appears.    So, select an 
analytical condition file.   
8 Save  
When you click this button after setting the parameters, the 
analytical conditions can be saved by overwriting.    In case of a 
new analysis method, clicking this button displays the “Save As” 
window.    You must enter a file name for saving in this window.   
9 Save As   
Click this...

2.2.2 
2 - 5 
When you click the Instrument tab, the window in Fig. 2-4 appears.   
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 2-4  Instrument Tab 
 
 
1 Scan mode   
Select a scan mode here.    In case of Synchronous, the abscissa 
of the graph stands for excitation wavelength.     
The unit indication of the abscissa becomes ‘EX/EM(nm).’   
2 Data mode   
Select a data mode.    The Luminescence mode is for measuring 
luminescence in the biological/chemical field....

2.2    Creating an Analysis Method 
2 - 6 
3 Chopping speed   
Select a chopping speed.    Setting is allowed when 
Phosphorescence is selected for Data mode.     
The Phosphorescence mode is for acquiring only phosphorescence 
after extinction of excitation beam while turning a chopper on the 
excitation side.    An increase in chopping speed makes the 
chopper turns faster.   
4 EM WL   
For the Excitation scan mode, enter the fixed wavelength on the 
emission side .    For the Synchronous scan mode,...

2.2.2 
2 - 7 
12 EX Slit   
Select a slit width on the excitation side.   
13 EM Slit   
Select a slit width on the emission side.   
14 PMT Voltage   
A function for controlling the voltage of the photomultiplier detector 
(photomultiplier tube).    As a reference, changing from 400 V to 
700 V increases the data value about two digits and changing from 
700 V to 950 V increases it about 1 digit.   
When a check mark is put for PMT Voltage 0-1000V, the 
photomultiplier voltage is settable within a range...

2.2    Creating an Analysis Method 
2 - 8 
19 Replicates  
Set the number of repeat measurements.   
An average spectrum can also be determined in repeat 
measurement.    Setting is available on the Processing tab (shown 
in Fig. 2-6).   
20 Cycle time   
Set a repetition interval.   
21 Range of corrected spectra   
Spectrum correction range is indicated.   
  

2.2.3 
2 - 9 
When selecting the Monitor tab, the window in Fig. 2-5 appears.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 2-5  Monitor Tab 
 
 
Assign the following parameters.   
 
1 Y-Axis Max (maximum)   
Enter a maximum value for Y-axis on the monitor window.   
2 Y-Axis Min (minimum)   
Enter a minimum value for Y-axis on the monitor window.   
3 Open data processing window after data acquisition   
Select whether or not to conduct data processing after sample...

2.2    Creating an Analysis Method 
2 - 10 
NOTE:  When the remaining memory capacity of PC in operation is 
very small (system resource is less than 20%), the data 
processing window will not open even if this item is turned ON.   
Either close the presently opened data processing window or 
restart the FL Solutions program.   
 
4 Open data processing window after acquisition (Overlay)   
Select whether or not to conduct data processing with spectra 
overlaid after sample measurement.    This setting...

2.2.4 
2 - 11 
On selection of the Processing tab, the window in Fig. 2-6 will be 
displayed.  
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 2-6  Processing Tab 
 
 
1 CAT (Computing for Averaging Transient)   
Valid when 2 or larger value is entered for Replicates at the 
Instrument tab.   
When a check mark is put at the head, an average spectrum is 
determined upon carrying out repeat measurement.   
This is especially effective for measuring samples with which the...

2.2    Creating an Analysis Method 
2 - 12 
 
 
 
Fig. 2-7   
 
 
3 Processing steps   
The processing sequence is displayed.    After measurement, data 
processing will be carried out in the order set here.   
How to Delete a Processing Method   
Select a processing method to be deleted and click the Left 
arrow button (
).    Then, the selected method disappears 
from the Processing steps field.   
 
Display and Change of Parameter   
When you click [+] in the Processing steps field, a parameter list...

2.2.4 
2 - 13 
4 Peak Finding:  Integration method  
Select an integration method for the peaks to be displayed in the 
data processing window.   
5 Peak Finding:  Threshold  
Enter the minimum photometric value difference (between peak 
and valley) for definition as a peak.   
 
 
 
 
6 Peak Finding:  Sensitivity  
Select the number of data points in the horizontal axis direction 
(abscissa).    For detecting sharp peaks, select a sensitivity value of 
“1.”    Or select “8” for detecting broad peaks....