Hitachi F7000 Instruction Manual

4.2.2 
4 - 15 
5 Manual calibration   
Put a check mark in this box for creating a calibration curve using 
factors.    Enter factors in response to the selected calibration type.     
6 Force curve through zero   
By putting a check mark in this box, a calibration curve is created 
so that its factor A
0 takes 0 automatically.     
7 Digit after decimal point   
Set the number of digits below decimal point for indicating the 
calculated concentration of the measured sample.     
The concentration value...

4.2    Creating an Analysis Method 
4 - 16 
When you click the Instrument tab, the window in Fig. 4-15 appears.   
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 4-15  Instrument Tab 
 
 
1 Data mode   
Select one of the three data modes.    The Luminescence mode is 
for measuring luminescence in the biological/chemical field.     
In this mode, only the sample side signal is acquired without 
performing ratio photometry.     
2 Chopping speed   
Select a chopping speed....

4.2.3 
4 - 17 
4 Fixed WL 
When ‘EX WL Fixed’ is selected in Wavelength mode, set the fixed 
wavelength on the excitation side.   
When ‘EM WL Fixed’ is selected in Wavelength mode, set the fixed 
wavelength on the emission side.   
5 WL (1 to 6)   
Make your entry for the number of wavelengths specified at the 
Quantitation tab.   
6 EX Slit   
Select a slit width on the excitation side.   
7 EM Slit   
Select a slit width on the emission side.   
8 PMT Voltage   
A function for controlling the voltage...

4.2    Creating an Analysis Method 
4 - 18 
Clicking the Standards tab opens the window shown in Fig. 4-16.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 4-16  Standards Tab 
 
 
1 Number of Samples   
For generating a sample table newly, enter the number of samples 
here, and then click the Update button.   
2 Standard Table   
This table gives a list of standards for sample measurement or 
calibration curve preparation.    The table contains the items listed 
below....

4.2.4 
4 - 19 
⎯ No.   
This is the number given to each sample.   
⎯ Sample   
Enter the name of each sample.     
⎯ Comment   
Enter comments on each sample.   
⎯ Concentration   
Enter the concentration of each sample.   
 
3 Update  
When clicking this button, a confirmation message appears.   
 
 
 
 
Clicking Yes will clear the presently entered sample names and 
comments, and table rows are formed by the entered number of 
samples.  
For adding a row below the final one in the sample list, click...

4.2    Creating an Analysis Method 
4 - 20 
When selecting the Monitor tab, the window in Fig. 4-17 appears.     
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 4-17  Monitor Tab 
 
 
Assign the following parameters.   
 
1 Y-Axis Max (maximum)   
Enter a maximum value for Y-axis on the monitor window.   
2 Y-Axis Min (minimum)   
Enter a minimum value for Y-axis on the monitor window.   
3 Open data processing window after data acquisition   
Select whether or not to...

4.2.6 
4 - 21 
Select the Report tab, and the window in Fig. 4-18 will appear.   
 
 
 
Fig. 4-18  Report Tab  
 
 
On this window, select the items to be contained in an analysis report.   
 
1 Output  
• Print Report   
A report will be printed.    For printing method, refer to 4.5.   
• Use Microsoft(R) Excel   
Data will be transferred to Microsoft(R) Excel.    For transferring 
method, refer to 7.13.   
• Use print generator sheet   
Select this method for using the report generator (option)....

4.2    Creating an Analysis Method 
4 - 22 
2 Print items (printable/transferable items) 
• Include date   
• Include method   
• Include calibration curve   
• Include standards data   
• Include calibration data   
• Include sample data   
3 Printer Font   
Click the Select button for changing the front to be used in the 
report.  
  

4.3.1 
4 - 23 
4.3  Defining Your Samples  
 
When “Use sample table” is not selected in Analysis Method, a sample 
must be defined in the following procedure before measurement.   
 
(1) Click the 
  button.    The window in Fig. 4-19 will appear.   
 
 
 
Fig. 4-19 
 
 
(2)  Make entries for Sample name and Comment.   
Maximum number of enterable characters (half-size)   
Sample name :  40 characters   
Comment : 255 characters  
 
For automatic saving, put a check mark in the “Auto File” box to...

4.3  Defining Your Samples 
4 - 24 
When “Use sample table” is selected in Analysis Method, samples must 
be defined in the following procedure before measurement.   
Open the monitor window and click the 
 button.  
When the Sample Table window in Fig. 4-20 appears, enter the sample 
name and comment for each sample.   
 
1 For creating a sample table newly, make entry for Number of 
Samples and click the Update button.    For instance, if there are 
five samples, enter “5” for Number of Samples and...