Hitachi F7000 Instruction Manual

4.2.2 
4 - 5 
Upon selection of the Quantitation tab, the window shown in Fig. 4-4 
appears.  
For the settable range of each parameter, refer to Appendix G.   
 
 
 
Fig. 4-4  Quantitation Tab 
 
 
1 Quantitation type   
Quantitation type is selectable among Wavelength, Peak area, 
Peak height, Derivative and Ratio.    Spectra measured by the Peak 
area, Peak height and Derivative methods can be checked, 
referring to 7.19.   
(a) Wavelength  
Upon selecting ‘None’ for Calibration type in this...

4.2    Creating an Analysis Method 
4 - 6 
(i)  One wavelength calculation   
This is the most generally used method.    When you 
specify one wavelength and enter it at the Instrument 
tab, a calibration curve and concentration are 
determined from the data I
1 at the specified wavelength 
as shown in Fig. 4-5 (a).   
 
 
 
Fig. 4-5 (a) 
 
 
(ii)  Two wavelength calculation   
When you specify two wavelengths and enter them at 
the Instrument tab, a calibration curve and 
concentration are calculated...

4.2.2 
4 - 7 
(iii)  Three wavelength calculation   
As shown in Fig. 4-5 (c), assuming I
1, I2 and I3 are the 
data values at three different wavelengths, a calibration 
curve and concentration are calculated through 
determination of I by the equation below.     
Photometry is made in the order of WL1, WL2 and WL3.     
 
I = I
2 -   
 
But the following relation must hold:   
WL1 < WL2 < WL3 or WL1 > WL2 > WL3   
 
 
 
Fig. 4-5 (c) 
 
 
(b) Peak area  
Enter the peak wavelength and its accuracy for...

4.2    Creating an Analysis Method 
4 - 8 
Measurement will be carried out within the wavelength range 
set at the Instrument tab.    According to the specified 
threshold level and sensitivity, peak wavelength is sought.   
The area of the peak found within a range of the entered 
Peak apex ± wavelength accuracy is calculated, thereby 
preparing a calibration curve.   
 
 
 
Fig. 4-6    Quantitation Tab (peak area) 
 
  

4.2.2 
4 - 9 
(c) Peak height  
Enter the peak wavelength and its accuracy for Peak apex.   
Measurement will be carried out within the wavelength range 
set at the Instrument tab.    According to the threshold level 
and sensitivity specified for the “Peak area” quantitation 
method, peak wavelength is sought.    A calibration curve is 
prepared using the height of the peak found within a range of 
the entered Peak apex ± wavelength accuracy.     
 
 
 
Fig. 4-7    Quantitation Tab (peak height) 
 
  

4.2    Creating an Analysis Method 
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(d) Derivative  
Enter peak wavelength (or wavelength to be used for 
calibration curve preparation) for Peak apex.     
Measurement will be carried out within the wavelength range 
set at the Instrument tab.    A calibration curve is created 
using the derivative value at the peak wavelength.     
Assign your values for derivative parameters; Derivative 
order, Smoothing order and Number of points.   
 
 
 
Fig. 4-8    Quantitation Tab (derivative) 
 
  

4.2.2 
4 - 11 
(e) Ratio  
Upon selecting Ratio, an input column for three factors (K-
factor 0, K-factor 1, K-factor 2) and a setting column for 
calculation method (+, -, ∗) are displayed.     
 
NOTE:  By this quantitation method, a calibration curve cannot 
be created.   
 
 
 
Fig. 4-9    Quantitation Tab (peak ratio) 
 
 
Calculation is made by the following equation.   
 
RATIO =    #K0 
 
# (calculation method):    +, -, ∗  
WL1, 2, 3  :  Specified wavelengths   
WL3  :  Background correcting...

4.2    Creating an Analysis Method 
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(a) None  
Mode in which calibration curve is not used.    When this 
mode is selected with Wavelength set for Quantitation type, 
multi-wavelength measurement at up to six wavelengths is 
possible.    However, no spectrum can be displayed.   
 
(b) 1st order  
This specifies the most generally used calibration curve.     
A linear calibration curve is to be created.    The formula of 
calibration curve is as follows.   
x = A
1y + A0  
Here, x is sample...

4.2.2 
4 - 13 
(d) 3rd order  
A calibration curve is created using a cubic curve.     
The formula of calibration curve is as follows.   
x = A
3y3 + A2y2 + A1y + A0 
Here, x is sample concentration and y is sample data.   
 
 
 
Fig. 4-12  3rd Order  
 
 
(e) Segmented  
A calibration curve created by connecting standard samples 
linearly according to the measured values of respective 
samples or input data.   
 
 
 
Fig. 4-13  Segmented 
 
 
Data 
K0Conc 
Data 
Conc
For the part which 
exceeds the...

4.2    Creating an Analysis Method 
4 - 14 
NOTE:  A proper calibration curve can be created when the 
photometric value increases or decreases 
monotonously versus the concentration value.    With a 
monotonic increase, the calibration curve is created with 
standard 1 (STD1) as origin.    And with a monotonic 
decrease, the calibration curve is created with STD1 
plotted on the photometric value axis and STD5 on the 
concentration axis.    In these examples, the number of 
standards is set at 5....