Hitachi F 2500 Manual
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2 - 18 2.5 Conducting a Wavelength Scan (1) There are two methods of starting the scan. (a) Select the Measure command from the Spectrophotometer menu. (b) Click the (measurement) button on the measurement toolbar. For halting the measurement midway, click the button on the toolbar. (2) Upon clicking the button, the window shown in Fig. 2-10 appears when using a sample table. Now click Yes. Fig. 2-10 (3) The dialog box as in Fig....
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2 - 19 (4) When automatic saving is set, data will be saved after completion of measurement. Upon opening its file, a data processing window will appear as in Fig. 2-12. Unless automatic saving is set, select the Save As command from the File menu and save data in file. Fig. 2-12 Data Processing Window (wavelength scan) (a) Spectrum The spectrum of a measured sample is traced. (b) Peak table Peak wavelength, peak height and valley wavelength are displayed. (a)...
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2 - 20 2.6 Data Printout Printout can be provided even when the function for automatic printout after measurement is not used. The following two procedures are available. (1) Select the Print command from the File menu. Or click the print button on the toolbar. The field active on the data processing window (e.g., (a) in Fig. 2-12) will be printed. What is displayed by clicking the Print Preview command in the File menu will be printed. Fig. 2-13...
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2 - 21 (2) Select the Report command in the Data menu. Or click the button on the toolbar. The Print Preview window will appear, so click Print. Select the items to be printed at the Report tab under Properties. Fig. 2-14 NOTE: If characters overflow from the print frame in repeat measurement or in overlaying, then change the printing orientation from vertical to horizontal. 2.6
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3 - 1 3. TIME SCAN 3.1 Flow of Operation This section explains the parameter setting and operating method for time scan. Measurement is performed in a flow shown below. Fig. 3-1 Operational Flow (time scan) End Start Method setting Click button. Measuring wavelength Scan time Sample name/comment input Click button. Measurement Data processing Rate calculation Peak detection Spectrum...
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3 - 2 3.2 Creating an Analysis Method Set the analytical conditions (method) prior to measurement. From the Edit menu, select the Method command or click the button. The dialog box shown in Fig. 3-2 will appear. Fig. 3-2 Analysis Method The following tabs are provided on the Analysis Method window for the time scan mode. These tabs are explained on the subsequent pages. • General • Instrument • Monitor • Processing • Report 3.2 Creating an Analysis...
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3 - 3 (1) General The parameters shown in Fig. 3-3 are provided under the General tab. Fig. 3-3 General Tab (a) Measurement (mode) Select a measurement mode from among the four listed below. • Wavelength scan • Time scan • Photometry • 3-D Scan (displayed when the ‘3-D Scan program’ (option) is installed in combination with Model F-2500) Here, select “Time scan.” For Wavelength scan, Photometry and 3-D Scan, refer to Sections 2, 4 and 5. (b) Operator...
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3 - 4 (e) Sampling • Standard When sample is exchanged manually. (f) Comments Enter a description or notes on measuring conditions. (g) Load Click this button to read out saved analytical conditions. The file opening window appears upon clicking, so select an analytical conditions file. (h) Save By clicking this button after setting the parameters for all five tabs, the analytical conditions are saved. Clicking this button overwrites parameters on the existing ones....
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3 - 5 (a) Data mode Select one of the following data modes. The Luminescence mode is for measuring luminescence in the biological/chemical field and only the sample side signal is acquired without performing ratio photometry. If phosphorescence life is shorter than 20 ms, “Phosphorescence short life” should be selected. In this case, unit time and measuring time are automatically set to ms and 20 ms. • Fluorescence • Luminescence • Phosphorescence (only with Model F-4500)...
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3 - 6 (e) Scan time Set a scan time. Model Input Range F-4500 10 to 3600 s 1000 to 9999 ms F-2500 10 to 9000 s According to the set scan time, data sampling interval is set as listed below. • Model F-4500 Measuring Time (s) Sampling Interval (ms) ~ 3 1 ~ 7 2 ~ 18 5 ~ 36 10 ~ 72 20 ~ 180 50 ~ 360 100 ~ 720 200 ~ 1800 500 ~ 3600 1000 In the Phosphorescence mode, the minimum sampling interval is 50 ms. It is fixed at 40 µs in...