Hitachi F 2500 Manual
Here you can view all the pages of manual Hitachi F 2500 Manual. The Hitachi manuals for Spectrophotometer are available online for free. You can easily download all the documents as PDF.
Page 131
4 - 26 (d) Calibration curve data Data such as determination coefficient and factors (A 0, A 1, A2, A3) of calibration curve are displayed. And the calibration type can be changed. Fig. 4-25-3 Calibration Curve Data The standard data can be input manually. This function is usable after measurement of standards. Either select Standard from the Edit menu of monitor window or click the button. A window as in Fig. 4-26 will appear. So enter values in the...
Page 132
4 - 27 Statistical calculation is performed for the measured sample. The results are displayed in the “Avg Conc” column of the data processing window as shown in Fig. 4-25-2. MEAN = CV = × 100 SD = () 1 NN X X 2 i 2 i − ∑∑− Here, N = number of standards MEAN = mean value SD = standard deviation CV = coefficient of variation (%) NOTE: “*****” is indicated for SD and CV values when they are nearly “0.” 4.4.2 Statistical Calculation N ΣXi...
Page 133
4 - 28 4.5 Printing Data Printout can be provided even when the function for automatic printout after measurement is not used. The following two procedures are available. (1) Select the Print command from the File menu. Or click the print button on the toolbar. The field active on the data processing window (e.g., (b) in Fig. 4-25) will be printed. What is displayed by clicking the Print Preview command in the File menu will be printed. Fig. 4-27...
Page 134
4 - 29 (2) Select the Report command in the Data menu. Or click the button on the toolbar. The Print Preview window will appear. So click the Print button. Select the items to be printed at the Report tab with the Properties command chosen in the Edit menu. The measuring parameters will also be printed. Fig. 4-28 4.5
Page 135
4 - 30 4.6 Remeasuring Standard Data and Sample Data Standard data and sample data can be remeasured at the mid- point of a series of measurements or after it. For remeasurement, implement the following procedure. (1) Open the monitor window after completion of measurement. (2) Click the data to be remeasured. Fig. 4-37 (3) Click Remeasure or select the Remeasure (E) command from the Spectrophotometer (S) menu. The dialog box in Fig. 4-38 will open....
Page 136
4 - 31 4.7 Manual Input of Standard Data The standard data can be input manually. This function is usable after measurement of standards. Either select Standard (T) from the Edit menu of monitor window or click the button. A window as in Fig. 4-39 will appear. So enter values in the Data column. After entry, click the OK or Update button. Then, the calibration curve is recalculated and displayed. Fig. 4-39 4.7
Page 137
4 - 32 4.8 Measurement Using Saved Calibration Curve Call a calibration curve in the following way. (1) Set measurement mode in Photometry. (2) From the File (F) menu, select the calibration curve load command. A window as shown below will appear. Select the file of the calibration curve to be used. Fig. 4-40 (3) When selecting the file, the window as shown in Fig. 4-41 will appear. The sample data section is blank. Fig. 4-41 4.8...
Page 138
4 - 33 (4) Edit a sample table. (5) Set a blank sample to carry out auto zero. From the Spectrophotometer (S) menu, select the Auto zero (Z) command. Or click the button. (6) From the Spectrophotometer (S) menu, select Scan (S) command. Or click the button on the toolbar. (7) Set and measure a sample. 4.8
Page 139
5 - 1 5. 3-D SCAN 5.1 Flow of Operation This section explains the parameter setting and operating method for 3-D Scan. For using the 3-D Scan mode in the Model F-2500, the optional ‘3-D Scan program’ needs to be installed. Measurement is made in the following procedure. Fig. 5-1 Operational Flow (3-D Scan) End Start Method setting Click button. Scan range Data mode Scan speed Sample name/comment...
Page 140
5 - 2 5.2 Creating an Analysis Method Set the analytical conditions (method) prior to measurement. (1) From the Edit menu, select the Method command or click the button. The dialog box shown in Fig. 5-2 will appear. Fig. 5-2 Analysis Method (2) Set the analysis method on this window. For details of each tab, refer to 5.1.2 onward. The following tabs are provided on the Analysis Method window for the 3-D Scan mode. These tabs are explained on the...