Hitachi F 2500 Manual
Here you can view all the pages of manual Hitachi F 2500 Manual. The Hitachi manuals for Spectrophotometer are available online for free. You can easily download all the documents as PDF.
Page 111
4 - 6 Fig. 4-5 Calculation Method 2) Peak area Input the peak wavelength and its accuracy. Measurement is made in the wavelength range specified at the Instrument tab. Peak wavelength is obtained and a calibration curve is prepared using the peak area of relevant peak. Select an integration method among Rectangular, Trapezoid and Romberg. Also set a threshold value and sensitivity for defining the peak. Fig. 4-6 Quantitation Tab (peak area)...
Page 112
4 - 7 3) Peak height Input the peak wavelength and its accuracy. Measurement is made in the wavelength range specified at the Instrument tab. Peak is determined within the accuracy set here and calculation is made in the height of that peak. Fig. 4-7 Quantitation Tab (peak height) 4) Derivative Input a peak wavelength (or wavelength to be used for calibration curve preparation). Measurement is made in the wavelength range specified at the Instrument tab and a...
Page 113
4 - 8 5) Ratio Upon selecting Ratio, an input column for three factors (K0, K1, K2) and a setting column for calculation method (+, -, *) are displayed. NOTE: By this quantitation method, a calibration curve cannot be created. Fig. 4-9 Quantitation Tab (peak ratio) Calculation is made by the following equation. RATIO = # (calculation method): +, -, * WL1, 2, 3 : Specified wavelengths WL3 : Background correcting wavelength A( ) : Measured value...
Page 114
4 - 9 1) None Mode in which calibration curve is not used. When this mode is selected with Wavelength set for Quantification type, multi-wavelength measurement at up to six wavelengths is possible. However, no spectrum can be displayed. 2) 1st order This specifies the most generally used calibration curve. A linear calibration curve is to be created. The formula of calibration curve is as follows. x = A 1y + A0 Here, x is sample concentration and y is sample data....
Page 115
4 - 10 3) 2nd order A calibration curve is created using a quadratic curve. The formula of calibration curve is as follows. x = A 2y2 + A1y + A0 Here, x is sample concentration and y is sample data. Fig. 4-11 2nd Order Data Ko Conc 4.2 Creating an Analysis Method
Page 116
4 - 11 4) 3rd order A calibration curve is created using a cubic curve. The formula of calibration curve is as follows. x = A 3y3 + A2y2 + A1y + A0 Here, x is sample concentration and y is sample data. Fig. 4-12 3rd Order Data Ko Conc 4.2
Page 117
4 - 12 5) Segmented A calibration curve created by connecting standard samples linearly according to the measured values of respective samples or input data. Fig. 4-13 Segmented NOTE: A proper calibration curve can be created when the photometric value increases or decreases monotonously versus the concentration value. With a monotonic increase, the calibration curve is created with standard 1 (STD1) as origin. And with a monotonic decrease, the calibration curve is...
Page 118
4 - 13 Calibration curve with monotonous increase Calibration curve with monotonous decrease (c) Number of wavelengths When “Calibration type” is “None,” up to six wavelengths are selectable, and with other calibration types, 1 to 3 wavelengths are selectable. (d) Concentration unit Input a concentration (in mg/mL for example) from the keyboard. (e) Manual calibration Put a check mark in this box for creating a calibration curve using factors. The number of...
Page 119
4 - 14 (f) Force curve through zero By putting a check mark in this box, a calibration curve is created so that its factor A 0 passes through “0” automatically. (g) Digit after decimal point Set the number of digits after decimal point for the calculated concentration of the measured sample. The concentration value is rounded off to the set number of digits. Input range: 0 to 3 (h) Lower concentration limit/Upper concentration limit This is used to set a normal value...
Page 120
4 - 15 (a) Data mode Select one of the following data modes. The Luminescence mode is for measuring luminescence in the biological/chemical field and only the sample side signal is acquired without performing ratio photometry. • Fluorescence • Luminescence • Phosphorescence (b) Wavelength mode Select a wavelength for creating a calibration curve. • EX WL Fixed • EM WL Fixed • Both WL Fixed (c) Wavelength (1 to 6) Input the number of wavelengths specified at the...