Hitachi F 2500 Manual

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1.11  Photometer Menu Commands  
 
The following commands are available in the Spectrophotometer 
menu.  
• Set Wavelength   
• Zero Adjust   
• Auto Pre-scan   
• Shutter Open/Close   
 
(1) Set Wavelength  
 
This command sets the excitation/emission wavelength to a 
specified wavelength.   
Click the Set Wavelength command or button, and the 
dialog box shown below will come up.   
 
 
 
 
(2) Auto Pre-scan  
 
This command automatically searches for an optimum 
excitation/emission wavelength...

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1.12  Utility Menu Commands  
 
The Utility menu contains the following commands: 
• Instrumental Response   
• Analog Output Gain 
• EX Power Monitor   
• Sens. Check   
• Lamp Usage   
• Lamp Off (only with Model F-2500)   
 
(1) Instrumental Response  
 
When you use this function.    You need the Spectral 
correction accessories kit (P/N 251-0126).       
 
(2)  Analog Output Gain   
 
Use this command for setting a data value corresponding to 
1 V of analog output.    For details, refer to...

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1.13    Termination of FL Solutions Program   
 
After completion of measurement, shut down the instrument in 
the following procedure.   
 
(1)  From the File (F) menu, select the Exit (X) command.   
 
 
 
Fig. 1-42 
 
 
Select Yes, and the FL Solutions program will then be 
terminated.   
 
(2)  Turn off the power switch of the spectrophotometer main 
unit.  
 
(3)  Click the Start button of the Windows 98 and select the 
Shut Down (U) item.    In the Shut Down Windows dialog 
box, select...

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2.  WAVELENGTH SCAN  
 
 
2.1  Flow of Operation  
 
This section explains the condition setting and operating method 
for wavelength scan.     
Measurement is made in the following procedure.   
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Fig. 2-1    Operational Flow (wavelength scan)   
End 
Start 
Method setting 
Click  button.  
 Scan range 
 Data mode 
 Scan speed     
 
Sample name/comment input
Click  button.  
Pre-scan 
Measurement 
Data processing 
...

2 - 2 
2.2    Creating an Analysis Method   
 
Set the analytical conditions (method) prior to measurement.   
From the Edit menu, select the Method command or click the 
        button.    The dialog box shown in Fig. 2-2 will appear.   
 
 
 
Fig. 2-2  Analysis Method 
 
 
The following tabs are provided on the Analysis Method window 
for the wavelength scan mode.    These tabs are explained on 
the subsequent pages.     
 General 
 Instrument 
 Monitor 
 Processing 
 Report  
2.2    Creating an...

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(1) General  
 
The parameters shown in Fig. 2-3 are provided under the 
General tab.   
 
 
 
Fig. 2-3  General Tab 
 
 
(a) Measurement (mode)  
Select a measurement mode from among the four 
listed below.   
 Wavelength scan  
 Time scan  
 Photometry  
  3-D Scan (displayed when the ‘3-D Scan program’ 
(option) is installed in combination with Model   
F-2500)  
 
Here, select “Wavelength scan.”    For Photometry, 
Time scan and 3-D Scan, refer to Sections 3, 4 and 5.   
 
(b) Operator...

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(e) Sampling  
 Standard  
When sample is exchanged manually.   
 
(f) Comments  
Enter a description or notes on measuring conditions.   
 
(g) Load  
Click this button to read out saved analytical conditions.   
The file opening window appears upon clicking, so 
select an analytical conditions file.   
 
(h) Save  
By clicking this button after setting the parameters for 
all five tabs, the analytical conditions are saved.   
Clicking this button overwrites parameters on the 
existing ones....

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(a) Scan mode  
Select one of the following scan modes.    In case of 
Synchronous, excitation wavelength appears on the 
abscissa of the graph.     
 Excitation  
 Emission  
 Synchronous  
 
(b) Data mode  
Select one of the following data modes.   
The Luminescence mode is for measuring 
luminescence in the biological/chemical field and only 
the sample side signal is acquired without performing 
ratio photometry.    The Phosphorescence mode is for 
acquiring only phosphorescence after...

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(e)  EX End WL   
This is the end wavelength for wavelength scan on the 
excitation side.    Input the longer wavelength side of 
measuring wavelength range.   
 
Model Input Range  
F-4500  210 to 900 nm   
F-2500  230 to 800 nm   
 
 
(f) EX WL  
An excitation wavelength can be input.    It is the end 
wavelength for wavelength scan on the excitation side.   
Input the longer wavelength side of measuring 
wavelength range.   
 
Model Input Range  
F-4500  200 to 900 nm   
F-2500  220 to 800 nm...

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(i) Scan speed  
Set a wavelength scan speed.  (Unit:  nm/min)  
 
F-4500 F-2500  
30000 3000 
12000 1500 
2400 300 
1200 60 
300 15 
60  
15  
 
 
For the Phosphorescence data mode, select one of the 
following values.   
 240 nm/min  
 60 nm/min 
 15 nm/min 
 
(j) Delay  
After pressing the Measure button, measurement is 
started following the delay time set here.    It is used for 
temperature stabilization, etc.    In repeat measurement, 
it is the time until the start of the first...