Hitachi F 2500 Manual

4 - 16 
(f) PMT Voltage  
A function for controlling the voltage of the 
photomultiplier detector (photomultiplier tube).     
Select one of the following values.    Changing from 
400 V to 700 V increases the data value about two 
digits and changing from 700 V to 950 V increases it 
about 1 digit.     
•  950 V (only with F-4500)   
• 700 V  
• 400 V 
 
(g)  Auto statistic cal. number   
Calculation is made for mean value, SD (standard 
deviation) and CV (coefficient of variation).    Set the 
number...

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(4) Standards  
 
Clicking the Standards tab opens the window shown in Fig. 
4-16.   
 
 
 
Fig. 4-16  Standards Tab 
 
 
(a) Sample table  
Sample table gives a list of standards for sample 
measurement or calibration curve preparation.     
This table contains the items listed below.     
 
NOTE:  The size of the sample table columns (cells) 
is changeable.    Bring the cursor to a 
boundary line between left and right table 
headers, and it changes into the one shown 
below.   
 
 
Now click...

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(b) Update  
By clicking this button, sample numbers are set by the 
entered number of samples.    The displayed sample 
names, comments, etc. are all cleared.   
 
(c) Insert  
When the initial screen is opened, the Insert button 
becomes active.    Click this button to insert data at the 
end of the sample list.   
 
(d) Delete  
Click the column of the sample No. to be deleted, and 
it becomes active.    Now click the Delete button and 
the item is deleted.     
 
(5) Monitor  
 
When...

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(c)  Open data processing window after data acquisition   
Select whether or not to conduct data processing after 
sample measurement.    When selected (check mark is 
applied), the data is saved automatically after 
measurement and its file is displayed as an icon on the 
window.    And by opening this icon, data processing 
such as peak detection is available.    When this 
function is not selected, data processing can be 
achieved by reading out the file via File-Open.  
 
(d)  Print report...

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(7) Output  
 
(a) Print Report  
A report will be printed.    For printing method, refer to 
4.5.  
 
(b)  Use Microsoft® Excel   
Data will be transferred to Microsoft® Excel.     
For transfer method, refer to 6.13.   
 
(c)  Use print generator 
Select this method for using the report generator 
(option).    For usage, refer to the instruction manual of 
the report generator.   
 
   
• Include date  
• Include method  
•  Include calibration curve   
•  Include standards data   
•  Include...

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4.3  Defining Your Samples  
 
When “Use Sample Table” has been set for the Analysis Method, 
the samples must be defined before measurement.   
 
(1)  Open the monitor window and select the Sample table 
command in the Edit menu or click the          button.   
 
(2)  When the Sample Table window in Fig. 4-19 appears, fill in 
the blank section.    For instance, if there are five samples, 
click the Insert button five times.    Or enter “5” for Number 
of Samples and click the Update button....

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4.4  Conducting Measurement  
 
First, create a working curve, and then proceed to sample 
measurement.   
 
(1)  Set the analysis method, and the window in Fig. 4-20 will 
appear provided a sample table is not used.   
 
 
 
Fig. 4-20 
 
 
When a sample table is used, the window in Fig. 4-21 will 
appear.  
 
 
 
Fig. 4-21 
4.4  Conducting Measurement  

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(2)  There are two methods of starting measurement.     
 
(a)  Select the Measure command from the 
Spectrophotometer menu.   
 
(b)  Click the     (measurement) button on the 
measurement toolbar.   
 
(3)  Standards will be measured.    When finished, the window 
in Fig. 4-22 will appear.    So click the OK button. 
 
 
 
Fig. 4-22 
 
 
(4)  Now measure the samples.    If a sample table is not used, 
click the Sample button.    To interrupt the measurement for 
other samples, click the...

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(6)  For conducting remeasurement after completing a series of 
measurements, click the data area to be remeasured as 
shown in Fig. 4-24 and then click the Remeasure button.   
For saving data after remeasurement, click the End button 
or     button.  The procedure is the same when a sample 
table is used.    Both standards and samples can be 
remeasured.   
 
 
 
Fig. 4-24 
 
 
(7)  For conducting a blank measurement, click the Blank 
button.  
 
(8)  Data is saved on completion of measurement....

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(a) Standard data  
The measurement results of standards used for 
preparing a calibration curve are displayed.   
 
 
 
Fig. 4-25-1  Standard Data 
 
 
(b) Calibration curve  
A calibration curve obtained from standard data is 
displayed.    The curve can also be traced.   
Concentration and photometric value are indicated at 
the top left of this field.   
 
(c) Sample data  
Sample data such as photometric value (Abs), 
concentration (Conc), mean value (Avg Conc), 
standard deviation (SD),...