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Hitachi F 2500 Manual

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Page 141

5 - 3 
(3) General  
 
The parameters shown in Fig. 5-3 are provided under the 
General tab.   
 
 
 
Fig. 5-3  General Tab 
 
 
(a) Measurement (mode)  
Select a measurement mode from among the four 
listed below.   
 Wavelength scan  
 Time scan  
 Photometry  
 3-D Scan  
Here, select “3-D Scan.”    For Wavelength scan, time 
scan and Photometry, refer to Sections 2, 3 and 4.   
 
(b) Operator  
Enter an operator name.     
 
(c) Instrument  
The model of the connected instrument is indicated....

Page 142

5 - 4 
(f) Load  
Click this button to read out saved analytical conditions.   
The file opening window appears upon clicking, so 
select an analytical conditions file.   
 
(g) Save  
By clicking this button after setting the parameters for 
all five tabs, the analytical conditions are saved.   
Clicking this button overwrites parameters on the 
existing ones.   
 
(h) Save As  
Click this button for renaming the set conditions for 
saving.    The “Save As” window will appear, so enter a 
file name....

Page 143

5 - 5 
(a) Data mode  
Select either of the following data modes.   
The Phosphorescence mode is for acquiring only 
phosphorescence after extinction of excitation beam 
while turning a chopper on the excitation side.     
 Fluorescence  
  Phosphorescence (with Model F-4500)   
 
(b)  EX Start WL   
This is the start wavelength for wavelength scan on the 
excitation side.    Input the shorter wavelength side of 
measuring wavelength range.   
 
Model Input Range  
F-4500  0, 200 to 850 nm 
F-2500  0,...

Page 144

5 - 6 
(f)  EM End WL   
This is the end wavelength for wavelength scan on the 
emission side.    Input the longer wavelength side of 
measuring wavelength range.     
 
Model Input Range  
F-4500  0, 200 to 900 nm 
F-2500  0, 200 to 800 nm  
 
 
(g)  EM Sampling interval   
In the 3-D Scan mode, emission spectrum 
measurement is repeated while shifting the excitation 
wavelength.    Therefore, a shorter sampling interval on 
the emission side will result in a longer measurement 
time.  
Input range:...

Page 145

5 - 7 
(j) EM Slit  
Select a slit width for the emission side.    Unit:    nm   
 
F-4500 F-2500 
1.0 2.5 
2.5 5.0 
5.0 10.0 
10.0 20.0 
20.0  
 
 
(k) PMT Voltage  
A function for controlling the voltage of the 
photomultiplier detector (photomultiplier tube).     
Select one of the following values.    Changing from 
400 V to 700 V increases the data value about two 
digits.   
  950 V (only with F-4500)   
 700 V  
 400 V 
 
(l) Response  
Select a response time from the following.   
 
F-4500...

Page 146

5 - 8 
(m) Corrected spectra  
A function for determining the spectrum inherent to a 
sample by correcting the photometer wavelength 
characteristic using the saved instrument parameters, 
following measurement with the instrument parameters 
for photometer control.    If there are no instrument 
parameters for the wavelength range to be measured, 
only the wavelength range that allows correction is 
scanned.    And if there is no wavelength area 
measurable, an error will occur.     
For using this...

Page 147

5 - 9 
(5) Monitor  
 
When selecting the Monitor tab, the window in Fig. 5-5 
appears.   
 
 
 
Fig. 5-5  Monitor Tab 
 
Assign the following parameters.   
 
(a)  Y-Axis Max (maximum)   
Input a maximum value for Y-axis on the monitor 
window.  
 
(b)  Y-Axis Min (minimum)   
Input a minimum value for Y-axis on the monitor 
window.  
 
(c) Contour interval  
Function for setting intervals of drawing contour lines.   
Input range:    0.01 to 1000   
 
(d)  Open data processing window after data...

Page 148

5 - 10 
NOTE:  When the remaining memory capacity of PC 
in operation is very small (system resource is 
less than 20%), the data processing window 
will not open even if this item is turned ON.   
Either close the presently opened data 
processing window or restart the FL 
Solutions program.   
 
(e)  Print report after data acquisition   
Select whether or not to print a report after sample 
measurement.    When selected (check mark is 
applied), printing will be made automatically after 
measurement....

Page 149

5 - 11 
(b) Threshold  
Set a detection limit for the photometric value axis of 
peak and valley.    Peaks or valleys below the setting 
will not be detected.    Input range is 0.001 to 1000.     
 
(c) Sensitivity  
Select the number of data points in the horizontal axis 
direction (abscissa).    Select a sensitivity of “1” for 
detecting sharp peaks.    Or select “8” for detecting 
broad peaks.   
 1  
 2 
 4 
 8 
 
(7) Report  
 
Select the Report tab, and the window in Fig. 5-7 will 
appear....

Page 150

5 - 12 
(c)  Use print generator sheet   
Select this method for using the report generator 
(option).    For usage, refer to the instruction manual of 
the report generator.   
 
(9) Orientation  
Selectable when Report is chosen for Print Method.   
Select a printing orientation.    Either Portrait or Landscape 
orientation is selectable.    When the Landscape orientation 
is selected, date and method can be printed.   
 
(a)  Printable (transferable) items   
1) Include date  
2) Include instrument...
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