Hitachi F 2500 Manual

8 - 4 
For remeasuring any of the standards after their measurements, 
click its data as shown in Fig. 8-6.    Then click the Remeasure 
button.  
 
 
 
 
Fig. 8-6 
 
 
A message box will open as in Fig. 8-7, so press the sipping 
lever.  
 
 
 
Fig. 8-7 
 
 
When a standard is remeasured, the calibration curve is 
recalculated and sample data is also recalculated based on the 
curve and then displayed.    To saved the recalculated data, click 
the End button or the          (save) button on the toolbar....

8 - 5 
Select the Measure command from the Spectrophotometer menu 
or click the          (measurement) button on the toolbar, and the 
window in Fig. 8-8 will open.   
 
 
 
Fig. 8-8 
 
 
Press the sipping lever and measurement starts.     
For measurement of the 2nd and subsequent standards, a 
message box as in Fig. 8-9 will open.    For remeasurement or 
for skipping measurement of the next standard, select the 
relevant item with the mouse (put a bullet in its radio button) and 
then click Yes.  When...

8 - 6 
On completion of standard measurement, the window in Fig.   
8-10 will open.   
 
 
 
Fig. 8-10 
 
 
By clicking OK with the mouse, a calibration curve is drawn and 
then samples can be measured.     
By clicking Cancel, the measurement is terminated, and the 
standards’ data is not saved.     
For remeasurement of the final standard, select “Repeat the last 
measurement” and click OK.    Set the final standard in place and 
press the sipping lever.    When repeat measurement has been 
set,...

8 - 7 
For measurement of the 2nd sample, a message box as in   
Fig. 8-12 opens.   
 
 
 
Fig. 8-12 
 
 
Press the sipping lever for measuring the 2nd sample.     
For skipping this sample (and measuring the 3rd sample), select 
“Skip this sample?” and click the Yes button with the mouse.   
For remeasurement, select “Repeat the last measurement?” and 
click Yes button with the mouse.    When repeat measurement 
has been set, measurement is started from the 1st one 
(Repl.No.1) for the 1st sample.   
In...

8 - 8 
For remeasurement after a series of measurements, click the 
data to be remeasured as in Fig. 8-14.    Then click the 
Remeasure button.   
 
 
Fig. 8-14 
 
 
A message box will open as in Fig. 8-15.    Press the sipping 
lever, and measurement starts.    When remeasuring a standard, 
the calibration curve is recalculated and sample data is also 
recalculated based on the curve and then displayed.    To save 
the recalculated data, click the     (Save) button. 
 
 
Fig. 8-15 
 
 
(1)  When not...

8 - 9 
8.2    Use of AS-3000 Intelligent Autosampler   
 
Set the rack parameters, injection volume and other items with 
the AS-3000 intelligent autosampler.    For details, refer to the 
instruction manual attached to the AS-3000.     
 
Select the Measure command from the Spectrophotometer menu 
or click the     (measurement) button on the toolbar.   
Then, press the START key on the AS-3000 intelligent 
autosampler.  Measurement will start.  
 
NOTE:  In measurement without using a sample table,...

8 - 10 
8.3  For Analog Output  
 
For connection, use the analog output terminal.    Select the 
Analog Output Gain command from the Utility menu.     
 
 
 
Fig. 8-17 
 
 
This is a setting of data value corresponding to analog output of 
1 V.   
Analog output value (V) becomes as follows.   
 
 Model F-4500 
Low :  (Data value - photometric value lower limit)/1024   
High :  (Data value - photometric value lower limit)/8192   
 
  Model F-2500   
Low :  (Data value)/1024   
High :  (Data...

 A -
1
APPENDIX A    DETAILS ON QUANTITATION 
 
 
A.1 Foreword 
In the Photometry mode of the F-2500 spectrophotometer, there 
are four calibration types. 
 
  Linear working curve 
  Quadratic working curve 
  Cubic working curve 
  Multiple segment working curve 
 
These are explained in more detail below. 
 
A.2  Linear Working Curve (1st order) 
 
Regression line is determined via least squares method from a 
maximum of 20 data.    The calculation formula is as follows : 
x = A1· y + A0 
A1 =...

 A -
2
where,  x :    Concentration of each sample (input value) 
y :    Data on each sample (measured value) 
n :    Number of samples 
 
A.4  Cubic Working Curve (3rd order) 
 
A cubic curve is determined via least squares method from a 
maximum of 20 data.    The calculation formula is as follows : 
X = A3 · Y
3 + A2 · y2 + A1 · y + A0 
where,  x :    Concentration of each sample (input value) 
y :    Data on each sample (measured value) 
n :    Number of samples 
 
A.5  Multiple Segment Working Curve...

 A -
3
 
 
(1)  A correct calibration curve can be created only when the 
measured value increases or decreases monotonically 
versus the concentration value.      Especially when the 
inclination is negative-going, be sure to measure a blank 
having a data value larger than the other standards.     
A curve not showing a monotonic increase will appear as in 
Fig. A-2.    And when the data value of the blank is small, 
regardless of a negative-going inclination, the curve will 
appear as in Fig. A-3....